Click here to switch to the map view.

The map label for this gene is ribE [H]

Identifier: 120598806

GI number: 120598806

Start: 2309147

End: 2309776

Strand: Direct

Name: ribE [H]

Synonym: Sputw3181_1992

Alternate gene names: 120598806

Gene position: 2309147-2309776 (Clockwise)

Preceding gene: 120598803

Following gene: 120598808

Centisome position: 49.04

GC content: 40.79

Gene sequence:

>630_bases
ATGTTTACAGGTATAGTTCAAGCCACCTGCGAGGTGGTTGCAATACATAAGAAAGATGGCCTAAATACCCTTGAGGTAGC
GTTTAAACCTGATTTGCATGAGGGACTTGCGATTGGTGCAAGTGTGGCAAATAACGGGGTATGTCTTACAGTGACTCAGG
TGGTTGATGATAGGGTATTTTTCGACGTAGTGGAAGAGACATTACGACTGACGAATCTCGCTAATTTGTCTGTAGGTCAA
TACGTTAATGTTGAACGCTCGTTAACTTTTGGTAGTGAAATTGGAGGACATATTCTTTCTGGCCATATCCATACAAAGGC
TAAAGTTACCAATATTAGCCACACAGAACAGCATTACGATTTGACATTGAGTGTTGAACCTAAATGGATGGACTATATAT
TTTATAAAGGATTTGTAGGTATAAATGGTTGCAGCCTAACGGTGGGTGAGGTGACAGAGACAAGTTTTATGCTGCACTTA
ATTCCAGAAACACTCAAGCTCACCAATTTAGGTCAGTGCAAGGTAGGTGATGAATTGAATATTGAAATCGACAGTCAAAC
GCAAGTCATCGTCGACACTGTCGAGCGTGTACTGGCCAAACGCTTGAGCGAGTATTCATTAAATAATTAA

Upstream 100 bases:

>100_bases
TTGGGCGATGAACACTGGCAGTGCAAGTTGGATTAAGCGTTTGGCTTGGAAGCCTGAATGTTTCATAAAATACAGCCTTT
AACGAATAAGCGAGTAATAA

Downstream 100 bases:

>100_bases
TAAATTTGCTCATCATCTGAGTCTACGTATAACTCAATCGTGCGCCGCAACTCATCTATATGTAAGCGTAAATGGATTGG
ATTACAGCAAATTCGACAAT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVFFDVVEETLRLTNLANLSVGQ
YVNVERSLTFGSEIGGHILSGHIHTKAKVTNISHTEQHYDLTLSVEPKWMDYIFYKGFVGINGCSLTVGEVTETSFMLHL
IPETLKLTNLGQCKVGDELNIEIDSQTQVIVDTVERVLAKRLSEYSLNN

Sequences:

>Translated_209_residues
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVFFDVVEETLRLTNLANLSVGQ
YVNVERSLTFGSEIGGHILSGHIHTKAKVTNISHTEQHYDLTLSVEPKWMDYIFYKGFVGINGCSLTVGEVTETSFMLHL
IPETLKLTNLGQCKVGDELNIEIDSQTQVIVDTVERVLAKRLSEYSLNN
>Mature_209_residues
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVFFDVVEETLRLTNLANLSVGQ
YVNVERSLTFGSEIGGHILSGHIHTKAKVTNISHTEQHYDLTLSVEPKWMDYIFYKGFVGINGCSLTVGEVTETSFMLHL
IPETLKLTNLGQCKVGDELNIEIDSQTQVIVDTVERVLAKRLSEYSLNN

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=202, Percent_Identity=54.950495049505, Blast_Score=215, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319733, Length=217, Percent_Identity=29.0322580645161, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23009; Mature: 23009

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVF
CCCCHHCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHCCHHH
FDVVEETLRLTNLANLSVGQYVNVERSLTFGSEIGGHILSGHIHTKAKVTNISHTEQHYD
HHHHHHHHHHHHCCCCCCCCEECCEECEECCCCCCCEEEECEEECEEEEECCCCCCCEEE
LTLSVEPKWMDYIFYKGFVGINGCSLTVGEVTETSFMLHLIPETLKLTNLGQCKVGDELN
EEEEECCCHHEEEEEECCEECCCCEEEECCCCCHHEEEEECCHHHHHCCCCCEECCCEEE
IEIDSQTQVIVDTVERVLAKRLSEYSLNN
EEECCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVF
CCCCHHCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHCCHHH
FDVVEETLRLTNLANLSVGQYVNVERSLTFGSEIGGHILSGHIHTKAKVTNISHTEQHYD
HHHHHHHHHHHHCCCCCCCCEECCEECEECCCCCCCEEEECEEECEEEEECCCCCCCEEE
LTLSVEPKWMDYIFYKGFVGINGCSLTVGEVTETSFMLHLIPETLKLTNLGQCKVGDELN
EEEEECCCHHEEEEEECCEECCCCEEEECCCCCHHEEEEECCHHHHHCCCCCEECCCEEE
IEIDSQTQVIVDTVERVLAKRLSEYSLNN
EEECCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]