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The map label for this gene is apbE [H]

Identifier: 120598743

GI number: 120598743

Start: 2225197

End: 2226024

Strand: Direct

Name: apbE [H]

Synonym: Sputw3181_1928

Alternate gene names: 120598743

Gene position: 2225197-2226024 (Clockwise)

Preceding gene: 120598742

Following gene: 120598744

Centisome position: 47.26

GC content: 44.69

Gene sequence:

>828_bases
ATGGCAAGCCCCTGTGAGCTGCTGATTGCCTGTGACCAGTTCGATATTGCTGATCATATGCTCAGTATGGCCGTGGCAGA
GGTTCAACGAATAGAGCAAAAGTATAGCCGTTTTATTGCACACAATATGCTCTGGAATGTAAATCATGCCCAAGGCCAAA
AACAATCAATTGATGAAGAAACTTGGCAATTATTGCAATTTGCTCAGCAGTGTTTTCAGTTAAGTGAGGGATTATTTGAT
ATTTCGGCAGGGCCTTTAATGGCACTTTGGCGATTCGAACCTAACACACCATTGCCTGCCCATATTGATATTCTGAGAAT
GAAAGCGTTTGTGGGATTTGAGCGTATAGACTTCACCTCACAATATTTAGTTATGCCAGCAGGAATGCAGTTAGATTTTG
GAGGGGTAGCAAAAGAATACGCAGTCGACAGCGTCGCCTATCAGCTCGCGGAGTCTTATCCCACTATCGCAGTATTAGTC
AATTTTGGGGGGGATATTGCTTGCCCAATTGCAAAAGCGACACCATGGCAAGTGGGCATTGAAGATCCACAACATCTTGA
TCATGCAGCTAAAGTACTTTCAATAAGGCATGGCGCACTCGCAACCAGTGGTGATACCCGCCGATTTATTGAAGTAGCAG
GAAAGCGTTACGGACATATTATTGATCCGCGAACGGGATATCCTGTGGTGTGCGCGCCACGTTCAGTGACTGTGTTGGGA
CCTAATTGTGTTACGGCTGGAATGCTTGCAACAATGGCGATGTTGCAAGGCGCTGATGCTGAAGTTTTTTTGACGCAACA
ATGTGTTAAATACAGTATTTTTAGATAG

Upstream 100 bases:

>100_bases
TCTTTATGAGAGATTTATAGTGCAGCCAACTTTTCCCCTTAAGAAAATACCATACACTTTGTCGCGTCGGGATTGGGGTT
ATATCGGTGAGTTTCGCGCC

Downstream 100 bases:

>100_bases
GATAAAGCGAATGCGCATTATGTTAGTTGAAGATAATGAATTACTTGCACAGGGTATTTGCCTTAGTTTGGCAAAGCTGG
GTATGCAAGTTGATCATCTC

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MASPCELLIACDQFDIADHMLSMAVAEVQRIEQKYSRFIAHNMLWNVNHAQGQKQSIDEETWQLLQFAQQCFQLSEGLFD
ISAGPLMALWRFEPNTPLPAHIDILRMKAFVGFERIDFTSQYLVMPAGMQLDFGGVAKEYAVDSVAYQLAESYPTIAVLV
NFGGDIACPIAKATPWQVGIEDPQHLDHAAKVLSIRHGALATSGDTRRFIEVAGKRYGHIIDPRTGYPVVCAPRSVTVLG
PNCVTAGMLATMAMLQGADAEVFLTQQCVKYSIFR

Sequences:

>Translated_275_residues
MASPCELLIACDQFDIADHMLSMAVAEVQRIEQKYSRFIAHNMLWNVNHAQGQKQSIDEETWQLLQFAQQCFQLSEGLFD
ISAGPLMALWRFEPNTPLPAHIDILRMKAFVGFERIDFTSQYLVMPAGMQLDFGGVAKEYAVDSVAYQLAESYPTIAVLV
NFGGDIACPIAKATPWQVGIEDPQHLDHAAKVLSIRHGALATSGDTRRFIEVAGKRYGHIIDPRTGYPVVCAPRSVTVLG
PNCVTAGMLATMAMLQGADAEVFLTQQCVKYSIFR
>Mature_274_residues
ASPCELLIACDQFDIADHMLSMAVAEVQRIEQKYSRFIAHNMLWNVNHAQGQKQSIDEETWQLLQFAQQCFQLSEGLFDI
SAGPLMALWRFEPNTPLPAHIDILRMKAFVGFERIDFTSQYLVMPAGMQLDFGGVAKEYAVDSVAYQLAESYPTIAVLVN
FGGDIACPIAKATPWQVGIEDPQHLDHAAKVLSIRHGALATSGDTRRFIEVAGKRYGHIIDPRTGYPVVCAPRSVTVLGP
NCVTAGMLATMAMLQGADAEVFLTQQCVKYSIFR

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=215, Percent_Identity=31.6279069767442, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 30443; Mature: 30312

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPCELLIACDQFDIADHMLSMAVAEVQRIEQKYSRFIAHNMLWNVNHAQGQKQSIDEE
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHH
TWQLLQFAQQCFQLSEGLFDISAGPLMALWRFEPNTPLPAHIDILRMKAFVGFERIDFTS
HHHHHHHHHHHHHHHCCCEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCC
QYLVMPAGMQLDFGGVAKEYAVDSVAYQLAESYPTIAVLVNFGGDIACPIAKATPWQVGI
CEEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCCCCEECC
EDPQHLDHAAKVLSIRHGALATSGDTRRFIEVAGKRYGHIIDPRTGYPVVCAPRSVTVLG
CCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEC
PNCVTAGMLATMAMLQGADAEVFLTQQCVKYSIFR
CCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure 
ASPCELLIACDQFDIADHMLSMAVAEVQRIEQKYSRFIAHNMLWNVNHAQGQKQSIDEE
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHH
TWQLLQFAQQCFQLSEGLFDISAGPLMALWRFEPNTPLPAHIDILRMKAFVGFERIDFTS
HHHHHHHHHHHHHHHCCCEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCC
QYLVMPAGMQLDFGGVAKEYAVDSVAYQLAESYPTIAVLVNFGGDIACPIAKATPWQVGI
CEEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCCCCEECC
EDPQHLDHAAKVLSIRHGALATSGDTRRFIEVAGKRYGHIIDPRTGYPVVCAPRSVTVLG
CCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEC
PNCVTAGMLATMAMLQGADAEVFLTQQCVKYSIFR
CCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]