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The map label for this gene is nifU [H]

Identifier: 120598678

GI number: 120598678

Start: 2147004

End: 2147387

Strand: Direct

Name: nifU [H]

Synonym: Sputw3181_1863

Alternate gene names: 120598678

Gene position: 2147004-2147387 (Clockwise)

Preceding gene: 120598677

Following gene: 120598679

Centisome position: 45.6

GC content: 44.01

Gene sequence:

>384_bases
ATGGCTTACAGTGAAAAAGTGATAGATCATTATGAGAACCCACGTAATGTAGGTTCCTTTGATAAAAACGATCCTTCGGT
CGTGACCGGTATGGTAGGCGCGCCTGCTTGCGGTGACGTGATGAAACTTCAGTTGAAAATTGGTGCTGATGGCATCATTC
AAGACGCTAAGTTCAAAACCTACGGTTGTGGTAGTGCCATTGCCTCTAGTTCACTGGTAACTGAGTGGGTCAAGGGTAAA
ACGATTGAACAAGCCGCAGCGATTAAAAATACTGATATCGCTGAGGAACTTGCGTTACCTCCAGTGAAAATTCACTGCTC
AATTTTGGCAGAAGATGCCATTAAAGCCGCTATTGATGAGTATAAATCGAAGCAAGCTCAGTAG

Upstream 100 bases:

>100_bases
CGTTATGGGAAATGTTTAAAGATGGTATCGACCTGAACCAAGTTCAGTGGGCACATCATTAGTTCTGACACTTAACAATA
GATAATTTTGGAGTAATACC

Downstream 100 bases:

>100_bases
CTTCTGGAGTTAAGATGGCGATTACAATGACCCCAGCGGCAACAGAGCGAGTCAGATCTTTCTTAGCAAATAGAGGCAAA
GGTATTGGCCTTCGCCTTGG

Product: scaffold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MAYSEKVIDHYENPRNVGSFDKNDPSVVTGMVGAPACGDVMKLQLKIGADGIIQDAKFKTYGCGSAIASSSLVTEWVKGK
TIEQAAAIKNTDIAEELALPPVKIHCSILAEDAIKAAIDEYKSKQAQ

Sequences:

>Translated_127_residues
MAYSEKVIDHYENPRNVGSFDKNDPSVVTGMVGAPACGDVMKLQLKIGADGIIQDAKFKTYGCGSAIASSSLVTEWVKGK
TIEQAAAIKNTDIAEELALPPVKIHCSILAEDAIKAAIDEYKSKQAQ
>Mature_126_residues
AYSEKVIDHYENPRNVGSFDKNDPSVVTGMVGAPACGDVMKLQLKIGADGIIQDAKFKTYGCGSAIASSSLVTEWVKGKT
IEQAAAIKNTDIAEELALPPVKIHCSILAEDAIKAAIDEYKSKQAQ

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=123, Percent_Identity=73.1707317073171, Blast_Score=196, Evalue=6e-51,
Organism=Homo sapiens, GI24307953, Length=123, Percent_Identity=72.3577235772358, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788878, Length=125, Percent_Identity=83.2, Blast_Score=221, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17543474, Length=125, Percent_Identity=71.2, Blast_Score=194, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=68.5483870967742, Blast_Score=183, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6324800, Length=124, Percent_Identity=66.1290322580645, Blast_Score=175, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21355597, Length=125, Percent_Identity=72, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011339
- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 13543; Mature: 13411

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSEKVIDHYENPRNVGSFDKNDPSVVTGMVGAPACGDVMKLQLKIGADGIIQDAKFKT
CCCCHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCC
YGCGSAIASSSLVTEWVKGKTIEQAAAIKNTDIAEELALPPVKIHCSILAEDAIKAAIDE
CCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHH
YKSKQAQ
HHHCCCC
>Mature Secondary Structure 
AYSEKVIDHYENPRNVGSFDKNDPSVVTGMVGAPACGDVMKLQLKIGADGIIQDAKFKT
CCCHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCC
YGCGSAIASSSLVTEWVKGKTIEQAAAIKNTDIAEELALPPVKIHCSILAEDAIKAAIDE
CCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHH
YKSKQAQ
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]