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The map label for this gene is iscS [H]

Identifier: 120598677

GI number: 120598677

Start: 2145751

End: 2146965

Strand: Direct

Name: iscS [H]

Synonym: Sputw3181_1862

Alternate gene names: 120598677

Gene position: 2145751-2146965 (Clockwise)

Preceding gene: 120598676

Following gene: 120598678

Centisome position: 45.57

GC content: 44.44

Gene sequence:

>1215_bases
ATGAAGCTTCCTATCTATTTAGATTATGCCGCCACCACGCCTGTCGATCCGAGAGTCGCGGAAAAGATGTTCCAATATAT
GACGATGGATGGCATCTTTGGCAACCCAGCCTCTCGTTCACATCGCTACGGCTGGCAAGCTGAAGAAGCGGTGGATATTG
CTCGCAATCAAGTGGCTGATTTGATCAATGCCGATCACCGTGAAATTGTGTTTACCTCTGGCGCGACTGAGTCAAATAAC
TTAGCCATCAAAGGTGTTGCTCATTTCTATAACAAGAAAGGCAAGCATATCATTACCAGCAAGACTGAACACAAAGCTGT
ACTCGATACCTGCCGTCAACTAGAACGTGAAGGCTTTGAAGTCACCTATTTAGAGCCCGCATCTAACGGCATTATTCCGA
TGGAACGTTTAGAAGCTGCTATGCGTGACGACACTATTCTTGTTAGCATCATGCACGTCAACAATGAAATCGGTGTGATC
CACGATATCGATGCAATCGGTGAGTTATGCCGCTCAAAAGGCATTATTTTCCATATGGATGCAGCACAAAGTGCAGGCAA
ATTGCCAATTGATGTGCAAGCCACAAAGGTCGATCTTATTTCAATCTCTGGTCATAAAATGTATGGCCCTAAAGGCATTG
GTGCCCTTTATGTTCGCCGTAAACCTCGTATTCGCTTAGAAGCGCAAATGCATGGTGGTGGACACGAACGTGGTATGCGC
AGTGGCACATTACCGACTCACCAAATAGTCGGTTTAGGTGAAGCCGCCGCGATAGCTAAAGCGGAAATGGCAACAGATAA
CGAACGTATTGGTCGTTTGCGCGATAAATTATGGAATGGCATCAAACATATTGAAGAGACGTATATTAACGGTGATCTAA
CGCAACGTTTTTGCGGTAGCCTCAATGTCAGCTTCAACTATGTTGAAGGTGAGTCTTTGATGATGGCCTTAAAAGATTTA
GCTGTTTCATCGGGTTCGGCTTGTACTTCAGCCAGCCTTGAACCGAGCTACGTATTACGTGCACTGGGTTTAAATGATGA
AATGGCACATAGCTCAATTCGTTTCTCCATTGGCCGTTTCACAACGGAAGAAGAAATCGATCATGCTATCGAAACTATTA
CACAATCTATTGATAAATTAAGAGAAATGTCCCCGTTATGGGAAATGTTTAAAGATGGTATCGACCTGAACCAAGTTCAG
TGGGCACATCATTAG

Upstream 100 bases:

>100_bases
AAGGAACAAAGAGTCATTTTATAGGCTCCTTGTTATACATAAAAATTATATTGTACGTTGTATGTAGCCTCGGCAGAGAC
TGCTAAGTACGGAGTGTGTT

Downstream 100 bases:

>100_bases
TTCTGACACTTAACAATAGATAATTTTGGAGTAATACCATGGCTTACAGTGAAAAAGTGATAGATCATTATGAGAACCCA
CGTAATGTAGGTTCCTTTGA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDIARNQVADLINADHREIVFTSGATESNN
LAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPASNGIIPMERLEAAMRDDTILVSIMHVNNEIGVI
HDIDAIGELCRSKGIIFHMDAAQSAGKLPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLPTHQIVGLGEAAAIAKAEMATDNERIGRLRDKLWNGIKHIEETYINGDLTQRFCGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDIARNQVADLINADHREIVFTSGATESNN
LAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPASNGIIPMERLEAAMRDDTILVSIMHVNNEIGVI
HDIDAIGELCRSKGIIFHMDAAQSAGKLPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLPTHQIVGLGEAAAIAKAEMATDNERIGRLRDKLWNGIKHIEETYINGDLTQRFCGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH
>Mature_404_residues
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDIARNQVADLINADHREIVFTSGATESNN
LAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPASNGIIPMERLEAAMRDDTILVSIMHVNNEIGVI
HDIDAIGELCRSKGIIFHMDAAQSAGKLPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLPTHQIVGLGEAAAIAKAEMATDNERIGRLRDKLWNGIKHIEETYINGDLTQRFCGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=59.7014925373134, Blast_Score=519, Evalue=1e-147,
Organism=Homo sapiens, GI156713448, Length=421, Percent_Identity=32.7790973871734, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=80.1980198019802, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=181, Percent_Identity=33.7016574585635, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789175, Length=231, Percent_Identity=29.4372294372294, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=61.0421836228288, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=33.5365853658537, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=58.1047381546135, Blast_Score=493, Evalue=1e-140,
Organism=Drosophila melanogaster, GI20129463, Length=405, Percent_Identity=58.5185185185185, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44936; Mature: 44936

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPASNGIIPMERLEAAMRDDTILVSIMHVNNEIGVIHDIDAIGELCRSKGIIFHMD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEC
AAQSAGKLPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEEEEECCCCHHCCCC
SGTLPTHQIVGLGEAAAIAKAEMATDNERIGRLRDKLWNGIKHIEETYINGDLTQRFCGS
CCCCCCHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
LNVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
CCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPASNGIIPMERLEAAMRDDTILVSIMHVNNEIGVIHDIDAIGELCRSKGIIFHMD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEC
AAQSAGKLPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEEEEECCCCHHCCCC
SGTLPTHQIVGLGEAAAIAKAEMATDNERIGRLRDKLWNGIKHIEETYINGDLTQRFCGS
CCCCCCHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
LNVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
CCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA