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The map label for this gene is topA [H]

Identifier: 120598575

GI number: 120598575

Start: 2023767

End: 2026409

Strand: Direct

Name: topA [H]

Synonym: Sputw3181_1758

Alternate gene names: 120598575

Gene position: 2023767-2026409 (Clockwise)

Preceding gene: 120598573

Following gene: 120598577

Centisome position: 42.98

GC content: 44.87

Gene sequence:

>2643_bases
ATGGGTAAATCGCTAGTTATTGTCGAATCGCCGGCCAAAGCTAAGACTATTAATAAATATCTTGGCAAAGAATTCATCGT
TAAATCGAGCGTAGGTCACATCCGAGATCTGCCGACATCATCGACTTCAGAAGGCAATGAAAAGGTTAAATCCGCTGCCG
AAGTTAAGAAGATGTCGCCAGAAGAAAAAGCTCAATATAAAAAGGTGAAGGATCAGCAAGCGCTGGTGTCTCGCATGGGC
GTTAACCCTGAAAAGGGCTGGGCGGCTAAGTACCAAATTTTGCCAGGTAAAGAAAAGGTCGTTAAAGAGTTACAGGCACT
TGCCGATTCTGCTGACCAAATCTATCTCGCAACCGACTTAGACCGTGAGGGAGAAGCTATCGCTTGGCATTTGCAAGAGG
TGATTGGTGGTGATCCTTCACGGTATCAACGTGTTGTTTTTAACGAAATTACTAAGTCTGCGATTCAAGATGCTTTTAGC
AAACCGTCGACACTCGACACTAACATGGTTAATGCCCAACAAGCCCGTCGCTTTTTAGACCGTGTGGTGGGCTTTATGGT
GTCACCACTCTTATGGAAAAAGGTGGCGCGAGGCTTGTCAGCGGGTAGAGTCCAATCCGTCGCAGTTCGCTTAGTGGTTG
AGCGAGAAAGTGAAATCAAAGCCTTTGTGCCAGAAGAATTTTGGGATGTACATGCCCAGTTAACTACACCCGCAGAAGAA
TCGCTGCGGATGGAAGTGGTTAAGCATTTAGATTCAGCTTTTGAACCCATCAATGAGCAACAAGCATTAGCTGCCGTGCA
GGCTTTATCTTCAGCGAGCTTTAAAGTCATTTCCCGCGAAGATAAAGCGACACAAAGTAAACCTTCAGCCCCTTTTATCA
CCTCAACTTTGCAGCAGGCTGCGAGTACCCGTTTAGGGTTTGGTGTTAAGAAAACCATGATGATGGCTCAGCGACTTTAT
GAAGCAGGCCATATCACTTATATGCGTACTGACTCAACCAATTTGAGCCAAGAAGCACTCGAAAGTGTGCGTGATATGAT
TGGTAAAGCATTTGGTGCCAAATATCTACCTGCAGAACCTATTCGTTATGGTTCAAAGGAAGGGGCACAAGAGGCTCATG
AGGCGATTCGTCCATCAAATGTCCACGTAGAGGCCACAGATCTAACCGATATGGAGCGTGACGCTCAGCGTTTGTATGAG
CTTATTTGGCGTCAGTTTGTTTCTTGTCAGATGACACCAGCACAATATGATGCAACCAAATTAACGGTGAAAGCGGGCGA
TTACGAATTAAAAGCCACGGGACGAACGCTGCGTTTTGATGGTTGGACACGGGTACAAACCGTGCTCAAGAAGAAAAATG
AAGAAGATAATACATTGCCATTTGTGTCACAGGGCGATGTATTAGCGTTGAAAGAGTTACAGCCTAAACAACATTTTACT
AAGCCGCCAGCACGATACAGTGAAGCTTCTTTAGTTAAAGAATTAGAAAAACGTGGCATTGGCCGCCCATCAACCTATGC
AACCATCATTTCAACGATTCAAGATCGTGGTTATGTAAAAGTCGAGAATCGTCGTTTCTATGCCGAAAAAATGGGTGAAA
TTGTCAGTGAACGATTAGTCAGTAGTTTTGCTGATTTAATGAGTTATGACTTTACCGCCAGCATGGAGCAAACATTAGAT
GATGTGGCTCGCGGCGAATTAGATTGGAAAAAAGTGCTTGATGGTTTCTATGCTGACTTTACTAAGCAATTAGAAAAAGC
GGAGCGTGATCCTGAAGAAGGTGGCATGCGTCTTAACCAAATGGTGATGACGGATATCAAGTGCCCAACTTGTGGTCGTC
CTATGGGGATCCGTACTGGTACAACTGGTGTTTTCCTTGGTTGCTCTGGTTATGAGTTGCCCCCCAAAGAACGCTGTAAG
ACGACGATGAACTTAACGCCTGGTGTTGAGGCGATTAGCGAAAACAGTGAAGATGCTGAAACAGATGCATTGCGTGCGAA
GCACCGCTGTGGTATTTGCGGCACGGCAATGGACAGTTACCTGATCGATGAGAAGCGTAAGTTACACGTTTGCGGTAACA
ATCCGATTTGTGAAGGTTATGAGGTTGAAGAAGGCCAATTTAAGATCAAAGGTTATGAAGGCCCATTGATTGAGTGTGAT
CGCTGTGGCCATGATATGGAATTAAAGAATGGTCGTTTTGGTAAATATTTTGGCTGTACTAACGAAGAATGTAAAAATAC
TCGTAAATTACTGAAAAATGGTGAGGCAGCGCCGCCAAAAGAAGATCCCATTTACTTACCAGAGCTCAAATGCAGCAAAT
CTGACGCGCATTTTGTCTTACGTGATGGTGCCGCAGGTATCTTCTTAGCGGCCAGTACTTTCCCTAAATCACGGGAAACG
AGAGCGCCATTAGTGGAAGAATTGGTGAAATACCGTGAATTGCTGTGGCCTAAGTACCAGTACCTTGCAGATGCGCCAAT
CTGTGATGACGAGGGCAATAAGGCTGCTGTGAAATTTAGCCGTAAAACAAAAGAGCAATACGTTGCTACTGAAGTTGACG
GTAAAGCGACAGGCTGGTCTGCGCATTACATCAATGGTAAGTGGGTGAGTGAGTCAACGGCTAAAAAAAGTAAAAAAGCG
TAA

Upstream 100 bases:

>100_bases
TGGTACATATAAAAGAGGGATTAACTTGAAACTAAGAGTACAACCCTCTATATATGGTGCCAATTTCCACCTTCTTGAGA
TTTTTGGCGCAAATTAAACT

Downstream 100 bases:

>100_bases
TTGATTAATTTTTTAGATAAAGCATATTTGCAAGTTGTGTCGTGTGAGTATGCTTGATTAGCTAAAATTTAATGGTTGCG
TGAGCTTATCGAGTTGTAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 880; Mature: 879

Protein sequence:

>880_residues
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSPEEKAQYKKVKDQQALVSRMG
VNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFS
KPSTLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAQLTTPAEE
SLRMEVVKHLDSAFEPINEQQALAAVQALSSASFKVISREDKATQSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLY
EAGHITYMRTDSTNLSQEALESVRDMIGKAFGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVHVEATDLTDMERDAQRLYE
LIWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRTLRFDGWTRVQTVLKKKNEEDNTLPFVSQGDVLALKELQPKQHFT
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLVSSFADLMSYDFTASMEQTLD
DVARGELDWKKVLDGFYADFTKQLEKAERDPEEGGMRLNQMVMTDIKCPTCGRPMGIRTGTTGVFLGCSGYELPPKERCK
TTMNLTPGVEAISENSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
RCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVLRDGAAGIFLAASTFPKSRET
RAPLVEELVKYRELLWPKYQYLADAPICDDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYINGKWVSESTAKKSKKA

Sequences:

>Translated_880_residues
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSPEEKAQYKKVKDQQALVSRMG
VNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFS
KPSTLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAQLTTPAEE
SLRMEVVKHLDSAFEPINEQQALAAVQALSSASFKVISREDKATQSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLY
EAGHITYMRTDSTNLSQEALESVRDMIGKAFGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVHVEATDLTDMERDAQRLYE
LIWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRTLRFDGWTRVQTVLKKKNEEDNTLPFVSQGDVLALKELQPKQHFT
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLVSSFADLMSYDFTASMEQTLD
DVARGELDWKKVLDGFYADFTKQLEKAERDPEEGGMRLNQMVMTDIKCPTCGRPMGIRTGTTGVFLGCSGYELPPKERCK
TTMNLTPGVEAISENSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
RCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVLRDGAAGIFLAASTFPKSRET
RAPLVEELVKYRELLWPKYQYLADAPICDDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYINGKWVSESTAKKSKKA
>Mature_879_residues
GKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSPEEKAQYKKVKDQQALVSRMGV
NPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFSK
PSTLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAQLTTPAEES
LRMEVVKHLDSAFEPINEQQALAAVQALSSASFKVISREDKATQSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYE
AGHITYMRTDSTNLSQEALESVRDMIGKAFGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVHVEATDLTDMERDAQRLYEL
IWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRTLRFDGWTRVQTVLKKKNEEDNTLPFVSQGDVLALKELQPKQHFTK
PPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLVSSFADLMSYDFTASMEQTLDD
VARGELDWKKVLDGFYADFTKQLEKAERDPEEGGMRLNQMVMTDIKCPTCGRPMGIRTGTTGVFLGCSGYELPPKERCKT
TMNLTPGVEAISENSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECDR
CGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVLRDGAAGIFLAASTFPKSRETR
APLVEELVKYRELLWPKYQYLADAPICDDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYINGKWVSESTAKKSKKA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=499, Percent_Identity=28.0561122244489, Blast_Score=160, Evalue=6e-39,
Organism=Homo sapiens, GI10835218, Length=599, Percent_Identity=25.7095158597663, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1787529, Length=871, Percent_Identity=71.2973593570609, Blast_Score=1293, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=565, Percent_Identity=24.6017699115044, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=586, Percent_Identity=24.2320819112628, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=513, Percent_Identity=26.3157894736842, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640096, Length=512, Percent_Identity=25.9765625, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24585251, Length=581, Percent_Identity=24.4406196213425, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98422; Mature: 98291

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSP
CCCCEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCH
EEKAQYKKVKDQQALVSRMGVNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECC
DREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFSKPSTLDTNMVNAQQARRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAQLTTPAEE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCHHHHCCCHHHHHHHHHCCCCHHH
SLRMEVVKHLDSAFEPINEQQALAAVQALSSASFKVISREDKATQSKPSAPFITSTLQQA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCHHHHHHHHH
ASTRLGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEALESVRDMIGKAFGAKYLPAEP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IRYGSKEGAQEAHEAIRPSNVHVEATDLTDMERDAQRLYELIWRQFVSCQMTPAQYDATK
CCCCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LTVKAGDYELKATGRTLRFDGWTRVQTVLKKKNEEDNTLPFVSQGDVLALKELQPKQHFT
EEEEECCEEEEECCCEEEECCHHHHHHHHHHCCCCCCCCCEECCCCEEEHHHCCCHHHCC
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
SSFADLMSYDFTASMEQTLDDVARGELDWKKVLDGFYADFTKQLEKAERDPEEGGMRLNQ
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHH
MVMTDIKCPTCGRPMGIRTGTTGVFLGCSGYELPPKERCKTTMNLTPGVEAISENSEDAE
HHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCEECCCCCCHHHHCCCCCCHH
TDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
HHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCEEEEECCCCCEEEHH
RCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEE
RDGAAGIFLAASTFPKSRETRAPLVEELVKYRELLWPKYQYLADAPICDDEGNKAAVKFS
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEEH
RKTKEQYVATEVDGKATGWSAHYINGKWVSESTAKKSKKA
HHHHHHHHHEECCCCCCCCEEEEECCEEECHHHHHHCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSP
CCCEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCH
EEKAQYKKVKDQQALVSRMGVNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECC
DREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFSKPSTLDTNMVNAQQARRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAQLTTPAEE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCHHHHCCCHHHHHHHHHCCCCHHH
SLRMEVVKHLDSAFEPINEQQALAAVQALSSASFKVISREDKATQSKPSAPFITSTLQQA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCHHHHHHHHH
ASTRLGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEALESVRDMIGKAFGAKYLPAEP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IRYGSKEGAQEAHEAIRPSNVHVEATDLTDMERDAQRLYELIWRQFVSCQMTPAQYDATK
CCCCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LTVKAGDYELKATGRTLRFDGWTRVQTVLKKKNEEDNTLPFVSQGDVLALKELQPKQHFT
EEEEECCEEEEECCCEEEECCHHHHHHHHHHCCCCCCCCCEECCCCEEEHHHCCCHHHCC
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
SSFADLMSYDFTASMEQTLDDVARGELDWKKVLDGFYADFTKQLEKAERDPEEGGMRLNQ
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHH
MVMTDIKCPTCGRPMGIRTGTTGVFLGCSGYELPPKERCKTTMNLTPGVEAISENSEDAE
HHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCEECCCCCCHHHHCCCCCCHH
TDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
HHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCEEEEECCCCCEEEHH
RCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEE
RDGAAGIFLAASTFPKSRETRAPLVEELVKYRELLWPKYQYLADAPICDDEGNKAAVKFS
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEEH
RKTKEQYVATEVDGKATGWSAHYINGKWVSESTAKKSKKA
HHHHHHHHHEECCCCCCCCEEEEECCEEECHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]