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The map label for this gene is estA [H]

Identifier: 120598513

GI number: 120598513

Start: 1953228

End: 1953899

Strand: Direct

Name: estA [H]

Synonym: Sputw3181_1696

Alternate gene names: 120598513

Gene position: 1953228-1953899 (Clockwise)

Preceding gene: 120598512

Following gene: 120598514

Centisome position: 41.48

GC content: 46.43

Gene sequence:

>672_bases
ATGTCAGCTTCTCTTGAGCGTATTGTCGTTGAGCCTAAGACGCCTGCTACGGCCGTGGTCATTTGGTTACATGGATTAGG
GGATTCGGGGGCAGGATTTGCCCCTGTTGTGCCAGCCTTAGGGTTACCCAGTCATCATAGCATTCGGTTTATTTTTCCCC
ATGCACCAGAGCAAGCTGTAACCATCAATGGCGGCTACGTCATGCGAGCTTGGTATGATATTAAAAGCATGGATTTACAT
GACAGAGCCGATATGCAAGGGGTTTTGGCATCAGAGTTACATGTTAACGCATTGATTAATGAACAAATCGCGGCAGGTAT
TCCGAGTGAACGCATTGTCTTGGCTGGATTTAGCCAAGGGGGTGTGATGAGCTTATTCTCAGGGCTACGTTTTGAAAAAA
GGCTTGCTGGCATCATGGCGTTATCTTGTTATTTACCGACAGCCGATGCGTTACCAGCGGACTTAAGTATGGCTAACCGT
AATACTCCGATATTACAACAACACGGTGTGCAGGATGATGTTGTCCCGCTATCTGCGGGAGCCTTAGCCAAAGATGTTTT
AATATCGGATGGATATCAAGTGCAGTGGCAAACTTACCCCATGGCACATTCGGTTATTCCTGCCCAGTTAAACGATATTC
GTCAGTGGTTATTACAGCAATTTGAAATGTAG

Upstream 100 bases:

>100_bases
GATGCGGAATAAAGTACGCCTGCGTCGCGTGTCTAACTTATAGATCTATCATCACTTTTTAGTTGATGAGTGTTTAATAT
CGAATTAGGGATTTTTTAGT

Downstream 100 bases:

>100_bases
CTAAGTCTAATTGCTGATAAAAACGGCATGCGGTAATGTCAATATTATCCATGCCTGTTCTTTTAAGTGAACAATAGTGA
TTTCATTTATTTCCTTCTAA

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSASLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPSHHSIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDLH
DRADMQGVLASELHVNALINEQIAAGIPSERIVLAGFSQGGVMSLFSGLRFEKRLAGIMALSCYLPTADALPADLSMANR
NTPILQQHGVQDDVVPLSAGALAKDVLISDGYQVQWQTYPMAHSVIPAQLNDIRQWLLQQFEM

Sequences:

>Translated_223_residues
MSASLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPSHHSIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDLH
DRADMQGVLASELHVNALINEQIAAGIPSERIVLAGFSQGGVMSLFSGLRFEKRLAGIMALSCYLPTADALPADLSMANR
NTPILQQHGVQDDVVPLSAGALAKDVLISDGYQVQWQTYPMAHSVIPAQLNDIRQWLLQQFEM
>Mature_222_residues
SASLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPSHHSIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDLHD
RADMQGVLASELHVNALINEQIAAGIPSERIVLAGFSQGGVMSLFSGLRFEKRLAGIMALSCYLPTADALPADLSMANRN
TPILQQHGVQDDVVPLSAGALAKDVLISDGYQVQWQTYPMAHSVIPAQLNDIRQWLLQQFEM

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=221, Percent_Identity=40.2714932126697, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI9966764, Length=204, Percent_Identity=39.7058823529412, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI20270341, Length=224, Percent_Identity=29.9107142857143, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508057, Length=210, Percent_Identity=35.2380952380952, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6323147, Length=211, Percent_Identity=34.1232227488152, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI45553055, Length=208, Percent_Identity=37.5, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553057, Length=208, Percent_Identity=37.5, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21357257, Length=208, Percent_Identity=37.5, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI161078179, Length=213, Percent_Identity=31.924882629108, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24176; Mature: 24045

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPSHHSIRFIFPHAPEQAV
CCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCEE
TINGGYVMRAWYDIKSMDLHDRADMQGVLASELHVNALINEQIAAGIPSERIVLAGFSQG
EECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GVMSLFSGLRFEKRLAGIMALSCYLPTADALPADLSMANRNTPILQQHGVQDDVVPLSAG
CHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCCCHHCCCCCCCHHHCCCCCCCEECCCC
ALAKDVLISDGYQVQWQTYPMAHSVIPAQLNDIRQWLLQQFEM
HHHHHHEECCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SASLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPSHHSIRFIFPHAPEQAV
CCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCEE
TINGGYVMRAWYDIKSMDLHDRADMQGVLASELHVNALINEQIAAGIPSERIVLAGFSQG
EECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GVMSLFSGLRFEKRLAGIMALSCYLPTADALPADLSMANRNTPILQQHGVQDDVVPLSAG
CHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCCCHHCCCCCCCHHHCCCCCCCEECCCC
ALAKDVLISDGYQVQWQTYPMAHSVIPAQLNDIRQWLLQQFEM
HHHHHHEECCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA