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The map label for this gene is ushA

Identifier: 120598514

GI number: 120598514

Start: 1954264

End: 1955982

Strand: Direct

Name: ushA

Synonym: Sputw3181_1697

Alternate gene names: 120598514

Gene position: 1954264-1955982 (Clockwise)

Preceding gene: 120598513

Following gene: 120598517

Centisome position: 41.51

GC content: 41.71

Gene sequence:

>1719_bases
ATGATAAATAAGCTTATTAAAGGATTAGTTGCAACGGCTGTGCTTGCAGCTCTCGCAGGTTGTAACAATAGTGATGATGA
TAAAATTCCAACAACATGTGCTGAAGCAGGGAGTGCGTGTAAAGCATTCACTATTTTACATACCAATGATAACCATGGTC
GTTTCTGGGAAAACAGTGACGGCGAATATGGTTTAGCAGCGCAGAAAACACTTGTAGACCAGATTCGTGCTGAAGTGAGC
AAGAATGGCGGTCAAACTTTATTATTATCGGGTGGTGATATCAATACTGGTGTACCAGAATCAGATTTACAAGATGCTAT
TCCTGATTTTACCGGTATGAATAAAATTGGTTATGACGCAATGGCCGTTGGTAACCATGAATTTGACAATCCGTTATCAG
TACTTGATATGCAGCGCCGTCTTGCAGAATTTCCAATGTTGGCTGCCAATATATACCGTAAAAATACCGATGGTAGTCAA
GGTGAGCGCTATTTTGATGCCTATAAAATTTTTAATGTTAACGGTTTAAAAATTGCTGTCATCGGTTTAACTACCGAAGA
TACTGCTAAAATTGGTAACCCTGAATTTATTAGCGATCTGATTTTTACTGATCCCAAAGTTGAAATTGCTAAGGTCATCA
AAGAAATTAAAGATGCCAAATCGGCTGATATTATTTTCGCAACGACACACATGGGCCACTATGCTGACGGTCAAAATGGC
AGTAACGCTCCAGGTGATGTTGCTCTCGCACGCGCGCTGAAAGAAGGTGATTTGCAAGTTGTTATTGGTGGCCACTCACA
GAATCCTGTTTGTATGGAATCTGGCAGTGAAAACAAAGTTTACGCCGATTTTAAGCCGGGTGATGAGTGTGCTCCAGATA
AGCAAAATGGTACGTGGATTATGCAAGCCCATGAATGGGGTAAATATGTAGGCCGAGCTGATTTTGAATATTTCAACGGT
GAGCTACATCTAGCAAGCTACAAACTTGTGCCTGTTAACATGCGTAAACTGGATGAGAAAGGTGCTAAAACTACCGAGTT
AGCTGGTGCTAAAATTGAACCAGATACTGAGTTAAAAGAGTTACTGTCTTACTATCAAGAAAAAGGGCAAGCTAAGCTAG
ATGAAGTGATTGCAACGACAGATGCCCTGTTAGATGGTGAACGCGCAAACGTGCGTAATAAACAAACTAACTTAGGCCGT
ATGTTAGCGATGGCGCAAAGTGGTAAAGTGTCTGCAGACTTCGGTGTGATGAACTCGGGAGGTGTGCGCGCCTCTATCAA
AGCAGGTAACATTACTTACCGTGACGTACTTACCGTACAACCGTTTGGTAACATGGTGACATTGAACGAAATGACTGGGA
CTGAAGTTGCTGCATACTTAGGCGCAGTGGGCAGTCTTCAAATTGGATCCGGTGGTTATGCACAAATCACAGGTGTGAAA
ATGACCATTGACTGTGTGGCTAAGACCGCCAATATCAGTCAAATCAATGGTAAAGATTTTAGTGCCGGAGCAACCTATAA
GTTCACTGTGCCGAGTTTTAATGCTGCTGGTGGTGACGGTTATCCGAAATTAGCAAGCCCAATTCAAACGGGTTATGTTG
ATGCTGATTTACTGTATACATTCTTAAAAGACAAACAAACGATCGTTGCTGCAGACTATAATCCTGTAGGCGACATCGTT
TATGAAAACTCATCAAGTGTTGAAGGTTGTAAACTGTAA

Upstream 100 bases:

>100_bases
ATTTCATCTTTATTATTTGCTATTATGCTCTGCGTCGAACTTGATAGAGTTCGAGACAAACTTTCTATGATAAAATAATT
AAAATCAAGGGGTTGATGTA

Downstream 100 bases:

>100_bases
CAACTAATCGATATTTGTTTAAGCATAAATGCCACTCACCTGAGTGGCATTTTTTACTTTTAATGATTCGAGTTATTACT
GTGATTTAGCTGAAATATAA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MINKLIKGLVATAVLAALAGCNNSDDDKIPTTCAEAGSACKAFTILHTNDNHGRFWENSDGEYGLAAQKTLVDQIRAEVS
KNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSVLDMQRRLAEFPMLAANIYRKNTDGSQ
GERYFDAYKIFNVNGLKIAVIGLTTEDTAKIGNPEFISDLIFTDPKVEIAKVIKEIKDAKSADIIFATTHMGHYADGQNG
SNAPGDVALARALKEGDLQVVIGGHSQNPVCMESGSENKVYADFKPGDECAPDKQNGTWIMQAHEWGKYVGRADFEYFNG
ELHLASYKLVPVNMRKLDEKGAKTTELAGAKIEPDTELKELLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGR
MLAMAQSGKVSADFGVMNSGGVRASIKAGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVGSLQIGSGGYAQITGVK
MTIDCVAKTANISQINGKDFSAGATYKFTVPSFNAAGGDGYPKLASPIQTGYVDADLLYTFLKDKQTIVAADYNPVGDIV
YENSSSVEGCKL

Sequences:

>Translated_572_residues
MINKLIKGLVATAVLAALAGCNNSDDDKIPTTCAEAGSACKAFTILHTNDNHGRFWENSDGEYGLAAQKTLVDQIRAEVS
KNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSVLDMQRRLAEFPMLAANIYRKNTDGSQ
GERYFDAYKIFNVNGLKIAVIGLTTEDTAKIGNPEFISDLIFTDPKVEIAKVIKEIKDAKSADIIFATTHMGHYADGQNG
SNAPGDVALARALKEGDLQVVIGGHSQNPVCMESGSENKVYADFKPGDECAPDKQNGTWIMQAHEWGKYVGRADFEYFNG
ELHLASYKLVPVNMRKLDEKGAKTTELAGAKIEPDTELKELLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGR
MLAMAQSGKVSADFGVMNSGGVRASIKAGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVGSLQIGSGGYAQITGVK
MTIDCVAKTANISQINGKDFSAGATYKFTVPSFNAAGGDGYPKLASPIQTGYVDADLLYTFLKDKQTIVAADYNPVGDIV
YENSSSVEGCKL
>Mature_572_residues
MINKLIKGLVATAVLAALAGCNNSDDDKIPTTCAEAGSACKAFTILHTNDNHGRFWENSDGEYGLAAQKTLVDQIRAEVS
KNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSVLDMQRRLAEFPMLAANIYRKNTDGSQ
GERYFDAYKIFNVNGLKIAVIGLTTEDTAKIGNPEFISDLIFTDPKVEIAKVIKEIKDAKSADIIFATTHMGHYADGQNG
SNAPGDVALARALKEGDLQVVIGGHSQNPVCMESGSENKVYADFKPGDECAPDKQNGTWIMQAHEWGKYVGRADFEYFNG
ELHLASYKLVPVNMRKLDEKGAKTTELAGAKIEPDTELKELLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGR
MLAMAQSGKVSADFGVMNSGGVRASIKAGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVGSLQIGSGGYAQITGVK
MTIDCVAKTANISQINGKDFSAGATYKFTVPSFNAAGGDGYPKLASPIQTGYVDADLLYTFLKDKQTIVAADYNPVGDIV
YENSSSVEGCKL

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=555, Percent_Identity=25.2252252252252, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1786687, Length=566, Percent_Identity=50, Blast_Score=559, Evalue=1e-160,
Organism=Drosophila melanogaster, GI19922444, Length=548, Percent_Identity=26.2773722627737, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922446, Length=524, Percent_Identity=26.3358778625954, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24654424, Length=524, Percent_Identity=26.3358778625954, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28573524, Length=556, Percent_Identity=24.1007194244604, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24652512, Length=428, Percent_Identity=24.7663551401869, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921980, Length=428, Percent_Identity=24.7663551401869, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161076508, Length=428, Percent_Identity=24.7663551401869, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24641187, Length=565, Percent_Identity=23.716814159292, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221329836, Length=500, Percent_Identity=22, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 61361; Mature: 61361

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00107 PROTEIN_KINASE_ATP ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKLIKGLVATAVLAALAGCNNSDDDKIPTTCAEAGSACKAFTILHTNDNHGRFWENSD
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCC
GEYGLAAQKTLVDQIRAEVSKNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDA
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCE
MAVGNHEFDNPLSVLDMQRRLAEFPMLAANIYRKNTDGSQGERYFDAYKIFNVNGLKIAV
EEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCEEEEE
IGLTTEDTAKIGNPEFISDLIFTDPKVEIAKVIKEIKDAKSADIIFATTHMGHYADGQNG
EEECCCCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
SNAPGDVALARALKEGDLQVVIGGHSQNPVCMESGSENKVYADFKPGDECAPDKQNGTWI
CCCCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCCCEEE
MQAHEWGKYVGRADFEYFNGELHLASYKLVPVNMRKLDEKGAKTTELAGAKIEPDTELKE
EEECHHHHHHCCCCCEEECCEEEEEEEEEEECHHHHHHHCCCCCHHCCCCCCCCCHHHHH
LLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGRMLAMAQSGKVSADFGVMNSG
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCEECCC
GVRASIKAGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVGSLQIGSGGYAQITGVK
CEEEEEEECCEEEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCEEEEEEEE
MTIDCVAKTANISQINGKDFSAGATYKFTVPSFNAAGGDGYPKLASPIQTGYVDADLLYT
EEEEEHHCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCHHHCCCCHHHHHH
FLKDKQTIVAADYNPVGDIVYENSSSVEGCKL
HHCCCCEEEEECCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MINKLIKGLVATAVLAALAGCNNSDDDKIPTTCAEAGSACKAFTILHTNDNHGRFWENSD
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCC
GEYGLAAQKTLVDQIRAEVSKNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDA
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCE
MAVGNHEFDNPLSVLDMQRRLAEFPMLAANIYRKNTDGSQGERYFDAYKIFNVNGLKIAV
EEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCEEEEE
IGLTTEDTAKIGNPEFISDLIFTDPKVEIAKVIKEIKDAKSADIIFATTHMGHYADGQNG
EEECCCCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
SNAPGDVALARALKEGDLQVVIGGHSQNPVCMESGSENKVYADFKPGDECAPDKQNGTWI
CCCCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCCCEEE
MQAHEWGKYVGRADFEYFNGELHLASYKLVPVNMRKLDEKGAKTTELAGAKIEPDTELKE
EEECHHHHHHCCCCCEEECCEEEEEEEEEEECHHHHHHHCCCCCHHCCCCCCCCCHHHHH
LLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGRMLAMAQSGKVSADFGVMNSG
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCEECCC
GVRASIKAGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVGSLQIGSGGYAQITGVK
CEEEEEEECCEEEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCEEEEEEEE
MTIDCVAKTANISQINGKDFSAGATYKFTVPSFNAAGGDGYPKLASPIQTGYVDADLLYT
EEEEEHHCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCHHHCCCCHHHHHH
FLKDKQTIVAADYNPVGDIVYENSSSVEGCKL
HHCCCCEEEEECCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA