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The map label for this gene is bioD

Identifier: 120598483

GI number: 120598483

Start: 1920066

End: 1920809

Strand: Direct

Name: bioD

Synonym: Sputw3181_1666

Alternate gene names: 120598483

Gene position: 1920066-1920809 (Clockwise)

Preceding gene: 120598482

Following gene: 120598491

Centisome position: 40.78

GC content: 46.64

Gene sequence:

>744_bases
ATGTTTTTTGTAACAGGAACAGATACCGACAGCGGTAAGACCTTGGTTTCATCGGCATTATTAACCGCAGTAAATCGTTT
AGGTAATATCGCCCATGCTGACTCCGCTGTGAGTATCCTTGCGGCTAATACTATGTTGACTTTAGGTGTTAAGCCTATTG
CCTCTGGCTGCACGCAAACCTCTGAAGGATTGCGTAATGCCGATGCGTTACAGTTGATGACGTCATCGTCACTAAAACTT
GCCTATCCCGTCATTAACCCATTTACCTTCGAACCTGCGATTGCACCGCACATTGCCGCCGCCCAAGTGGGCGTGAGTTT
AGCGCCTGAAGTTATTATTGAAAGTTTAGATTTAGTGGCATTTGCTCAAGCGGATTTTTGCTTGATTGAGGGCGCGGGGG
GCTGGCGTTTACCCTTAGGTGATGGCAGGTATCTTTCGGAAGTCGTACAGAGATTAGAACTGCCTGTGATACTCGTGGTT
GGCATGAAACTCGGTTGCTTAAATCATGCATTGCTGACTCAAGAGGCGATATTGGCCGATGGGCTTAAGATTGCAGGTTG
GGTCGCGAATAGTGTCGATCCGAACATGAGTATGTTTGAGGAGAATCTGACGAGTCTTAAAGAGATGATGACTGCACCTT
TCCTCGGTTGTGTGCCATTTCTCGATGAGCCCTCGGCTGACAATGCGGCGAATTTTTTACATTGTGAAGCATTACTAAAG
CGGGTTATTCCTGAAGCTAATTAA

Upstream 100 bases:

>100_bases
ACCGCAAGGCTTACCTCTGACTTATCAAATTGCACAAATTCGTGCGCGTCGATTAGGTTAACCGAATCGGATAACGGGGT
AGTTTAATAAAGGATCTAAT

Downstream 100 bases:

>100_bases
CCGGAGTAGCTCGGCATGTTGCTTTTAGTGTTATGCGAGCTGTATTTAGCAAAAGGTCACACCTGACCCTTTGCTAACAG
TATGAAAATTAAGATAAGCC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MFFVTGTDTDSGKTLVSSALLTAVNRLGNIAHADSAVSILAANTMLTLGVKPIASGCTQTSEGLRNADALQLMTSSSLKL
AYPVINPFTFEPAIAPHIAAAQVGVSLAPEVIIESLDLVAFAQADFCLIEGAGGWRLPLGDGRYLSEVVQRLELPVILVV
GMKLGCLNHALLTQEAILADGLKIAGWVANSVDPNMSMFEENLTSLKEMMTAPFLGCVPFLDEPSADNAANFLHCEALLK
RVIPEAN

Sequences:

>Translated_247_residues
MFFVTGTDTDSGKTLVSSALLTAVNRLGNIAHADSAVSILAANTMLTLGVKPIASGCTQTSEGLRNADALQLMTSSSLKL
AYPVINPFTFEPAIAPHIAAAQVGVSLAPEVIIESLDLVAFAQADFCLIEGAGGWRLPLGDGRYLSEVVQRLELPVILVV
GMKLGCLNHALLTQEAILADGLKIAGWVANSVDPNMSMFEENLTSLKEMMTAPFLGCVPFLDEPSADNAANFLHCEALLK
RVIPEAN
>Mature_247_residues
MFFVTGTDTDSGKTLVSSALLTAVNRLGNIAHADSAVSILAANTMLTLGVKPIASGCTQTSEGLRNADALQLMTSSSLKL
AYPVINPFTFEPAIAPHIAAAQVGVSLAPEVIIESLDLVAFAQADFCLIEGAGGWRLPLGDGRYLSEVVQRLELPVILVV
GMKLGCLNHALLTQEAILADGLKIAGWVANSVDPNMSMFEENLTSLKEMMTAPFLGCVPFLDEPSADNAANFLHCEALLK
RVIPEAN

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=231, Percent_Identity=46.7532467532467, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI87081944, Length=242, Percent_Identity=36.3636363636364, Blast_Score=153, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 26066; Mature: 26066

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFVTGTDTDSGKTLVSSALLTAVNRLGNIAHADSAVSILAANTMLTLGVKPIASGCTQT
CEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHEECCHHHHHCCHHH
SEGLRNADALQLMTSSSLKLAYPVINPFTFEPAIAPHIAAAQVGVSLAPEVIIESLDLVA
HHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHH
FAQADFCLIEGAGGWRLPLGDGRYLSEVVQRLELPVILVVGMKLGCLNHALLTQEAILAD
EECCCEEEEECCCCEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHC
GLKIAGWVANSVDPNMSMFEENLTSLKEMMTAPFLGCVPFLDEPSADNAANFLHCEALLK
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHH
RVIPEAN
HHCCCCC
>Mature Secondary Structure
MFFVTGTDTDSGKTLVSSALLTAVNRLGNIAHADSAVSILAANTMLTLGVKPIASGCTQT
CEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHEECCHHHHHCCHHH
SEGLRNADALQLMTSSSLKLAYPVINPFTFEPAIAPHIAAAQVGVSLAPEVIIESLDLVA
HHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHH
FAQADFCLIEGAGGWRLPLGDGRYLSEVVQRLELPVILVVGMKLGCLNHALLTQEAILAD
EECCCEEEEECCCCEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHC
GLKIAGWVANSVDPNMSMFEENLTSLKEMMTAPFLGCVPFLDEPSADNAANFLHCEALLK
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHH
RVIPEAN
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA