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The map label for this gene is bioF [H]

Identifier: 120598481

GI number: 120598481

Start: 1917867

End: 1919078

Strand: Direct

Name: bioF [H]

Synonym: Sputw3181_1664

Alternate gene names: 120598481

Gene position: 1917867-1919078 (Clockwise)

Preceding gene: 120598480

Following gene: 120598482

Centisome position: 40.73

GC content: 48.84

Gene sequence:

>1212_bases
ATGAGTTTTCAGCTCGATGCGAAAATAGCTGCTCGTCAACAGGCTTTGTTTGAGCAAGGACTGCTGAGACAACGTAACGC
CTTATGCACAGACAGTTTATGCGCTGATAGCATAGGAGATTTAAGTCCTCGATTTTTATTCGAGGGGGGTAGCTATCTTA
ATTTCAGCAGCAACGATTATTTAGGTTTATCCCGTAACCCCGCGTTGATTGAAGCGCTGCACAGTGCGGCGCTTCAATAT
GGGGTAGGCAGCGGCGCATCCCCCTTAGTGACAGGTTACAGTAAGGCACATTTTACCCTTGAGCAAAGGCTCTGTGAACT
GACAGGTCACGAAGCGGCGTTGCTCTTTAGTTCAGGTTTTAGTGCGAATACTGCCTTGATGAAAACCCTGTTTGATAAAC
AGGATATAGTCGTTGCCGATAAGCTAGTGCATGCCTCTATTATTGATGGTTTATGCGATAGTGGCGCCGATCTTAAGCGT
TTCCTACATAATTGCCTCGAGAGCGCCGAGCGGCTCCTTAGCCGTAACTCTGCATCGGCGCTTATCACCGAAAGCGTATT
TAGTATGGATGGAGACTGTGCGCCGGTCGCCGCATTATCGACCCTCTGCCGTCAACATCATACTTGGTTGATAGTCGATG
ATGCCCATGGTTTTGGTGTACTGCCCGGGTTTGATGCTGGCAAAGCTGCCGAACTTATGGATGTGCAAGTGGTAACCTTT
GGTAAGGCTTTAGGTTGTCAGGGCGCTGCGGTTTTAGGTAGCAAAGCCTTGATCGACTTTTTGGTGAGTAATGCCCGTGA
ATACATTTATTCTACGGCACTTTCGCCCGTAAATGCAGCGCTGGCATTGGCCGCAACTGAGCACTGTGTTGCACACCCTG
AACTGCGGCTAAAGCTTCTTGAAAATATTCAATTATTTAAACAGTTATGTCTTGAGGCAAAAGTGCCGTTACTCGGTTCA
GATACCGCTATTCAGCCAGTGATCATTGGCGATTCCCAGCAAACTTTAAGGATTTCAAGTGCGCTAAAGGAGAGAGGGAT
TTGGGTTGGGGCGATTCGTCCACCCACTGTGCCTGTTGGATCCGCGCGGCTTCGCATTACGTTAACGGCATCCCATAGTG
CCAGTGATATTGCCTACTGTGTCAATGTGCTCGCTCAGGTGCTGGATAATGCTCATCTGCCTTCGGTCAGCGAGCGTGAA
CGTCCAGAGTAA

Upstream 100 bases:

>100_bases
GTGGCAACCCTTGATGATGAACAGGCGGTGCTGGCTAAAGCGGCTGCCCATCAAGATAAATCAACAGCGCAGTTCTATGA
TGCTGCGGCGCTGTAATTAA

Downstream 100 bases:

>100_bases
TCTCGTTTGAGCGATGTTATCTTTTATGCCGTTTCTTTGTCGTTTATGACAAAAGTCCATTATTCATGCTGACTTTGGAC
TGTACCAATATGAGTATCGT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSFQLDAKIAARQQALFEQGLLRQRNALCTDSLCADSIGDLSPRFLFEGGSYLNFSSNDYLGLSRNPALIEALHSAALQY
GVGSGASPLVTGYSKAHFTLEQRLCELTGHEAALLFSSGFSANTALMKTLFDKQDIVVADKLVHASIIDGLCDSGADLKR
FLHNCLESAERLLSRNSASALITESVFSMDGDCAPVAALSTLCRQHHTWLIVDDAHGFGVLPGFDAGKAAELMDVQVVTF
GKALGCQGAAVLGSKALIDFLVSNAREYIYSTALSPVNAALALAATEHCVAHPELRLKLLENIQLFKQLCLEAKVPLLGS
DTAIQPVIIGDSQQTLRISSALKERGIWVGAIRPPTVPVGSARLRITLTASHSASDIAYCVNVLAQVLDNAHLPSVSERE
RPE

Sequences:

>Translated_403_residues
MSFQLDAKIAARQQALFEQGLLRQRNALCTDSLCADSIGDLSPRFLFEGGSYLNFSSNDYLGLSRNPALIEALHSAALQY
GVGSGASPLVTGYSKAHFTLEQRLCELTGHEAALLFSSGFSANTALMKTLFDKQDIVVADKLVHASIIDGLCDSGADLKR
FLHNCLESAERLLSRNSASALITESVFSMDGDCAPVAALSTLCRQHHTWLIVDDAHGFGVLPGFDAGKAAELMDVQVVTF
GKALGCQGAAVLGSKALIDFLVSNAREYIYSTALSPVNAALALAATEHCVAHPELRLKLLENIQLFKQLCLEAKVPLLGS
DTAIQPVIIGDSQQTLRISSALKERGIWVGAIRPPTVPVGSARLRITLTASHSASDIAYCVNVLAQVLDNAHLPSVSERE
RPE
>Mature_402_residues
SFQLDAKIAARQQALFEQGLLRQRNALCTDSLCADSIGDLSPRFLFEGGSYLNFSSNDYLGLSRNPALIEALHSAALQYG
VGSGASPLVTGYSKAHFTLEQRLCELTGHEAALLFSSGFSANTALMKTLFDKQDIVVADKLVHASIIDGLCDSGADLKRF
LHNCLESAERLLSRNSASALITESVFSMDGDCAPVAALSTLCRQHHTWLIVDDAHGFGVLPGFDAGKAAELMDVQVVTFG
KALGCQGAAVLGSKALIDFLVSNAREYIYSTALSPVNAALALAATEHCVAHPELRLKLLENIQLFKQLCLEAKVPLLGSD
TAIQPVIIGDSQQTLRISSALKERGIWVGAIRPPTVPVGSARLRITLTASHSASDIAYCVNVLAQVLDNAHLPSVSERER
PE

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=348, Percent_Identity=31.8965517241379, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI284448556, Length=345, Percent_Identity=31.8840579710145, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4502025, Length=338, Percent_Identity=32.8402366863905, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI40316939, Length=338, Percent_Identity=32.8402366863905, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI83977444, Length=335, Percent_Identity=31.9402985074627, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI83977440, Length=335, Percent_Identity=31.9402985074627, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI83977442, Length=335, Percent_Identity=31.9402985074627, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4758668, Length=378, Percent_Identity=26.4550264550265, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI119220554, Length=400, Percent_Identity=24.25, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI5454084, Length=257, Percent_Identity=26.4591439688716, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786993, Length=342, Percent_Identity=45.3216374269006, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1790046, Length=351, Percent_Identity=32.7635327635328, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71994529, Length=344, Percent_Identity=31.6860465116279, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI32566772, Length=361, Percent_Identity=24.9307479224377, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560914, Length=364, Percent_Identity=24.7252747252747, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560912, Length=364, Percent_Identity=24.7252747252747, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71982625, Length=314, Percent_Identity=23.5668789808917, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982617, Length=314, Percent_Identity=23.5668789808917, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320438, Length=379, Percent_Identity=31.6622691292876, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6320267, Length=400, Percent_Identity=26, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323954, Length=224, Percent_Identity=27.2321428571429, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24662918, Length=345, Percent_Identity=32.463768115942, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI17137420, Length=351, Percent_Identity=31.0541310541311, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17136286, Length=365, Percent_Identity=24.3835616438356, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653280, Length=290, Percent_Identity=25.1724137931034, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653276, Length=290, Percent_Identity=25.1724137931034, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653278, Length=290, Percent_Identity=25.1724137931034, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 43104; Mature: 42973

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQLDAKIAARQQALFEQGLLRQRNALCTDSLCADSIGDLSPRFLFEGGSYLNFSSNDY
CCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCCCEEECCCCCC
LGLSRNPALIEALHSAALQYGVGSGASPLVTGYSKAHFTLEQRLCELTGHEAALLFSSGF
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SANTALMKTLFDKQDIVVADKLVHASIIDGLCDSGADLKRFLHNCLESAERLLSRNSASA
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
LITESVFSMDGDCAPVAALSTLCRQHHTWLIVDDAHGFGVLPGFDAGKAAELMDVQVVTF
HHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEH
GKALGCQGAAVLGSKALIDFLVSNAREYIYSTALSPVNAALALAATEHCVAHPELRLKLL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
ENIQLFKQLCLEAKVPLLGSDTAIQPVIIGDSQQTLRISSALKERGIWVGAIRPPTVPVG
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
SARLRITLTASHSASDIAYCVNVLAQVLDNAHLPSVSERERPE
CCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFQLDAKIAARQQALFEQGLLRQRNALCTDSLCADSIGDLSPRFLFEGGSYLNFSSNDY
CEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCCCEEECCCCCC
LGLSRNPALIEALHSAALQYGVGSGASPLVTGYSKAHFTLEQRLCELTGHEAALLFSSGF
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SANTALMKTLFDKQDIVVADKLVHASIIDGLCDSGADLKRFLHNCLESAERLLSRNSASA
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
LITESVFSMDGDCAPVAALSTLCRQHHTWLIVDDAHGFGVLPGFDAGKAAELMDVQVVTF
HHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEH
GKALGCQGAAVLGSKALIDFLVSNAREYIYSTALSPVNAALALAATEHCVAHPELRLKLL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
ENIQLFKQLCLEAKVPLLGSDTAIQPVIIGDSQQTLRISSALKERGIWVGAIRPPTVPVG
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
SARLRITLTASHSASDIAYCVNVLAQVLDNAHLPSVSERERPE
CCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]