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The map label for this gene is focA [H]

Identifier: 120598450

GI number: 120598450

Start: 1865285

End: 1866898

Strand: Direct

Name: focA [H]

Synonym: Sputw3181_1633

Alternate gene names: 120598450

Gene position: 1865285-1866898 (Clockwise)

Preceding gene: 120598446

Following gene: 120598452

Centisome position: 39.62

GC content: 44.92

Gene sequence:

>1614_bases
ATGAAGGCATCACATTATGATGCTCATATGAGGGCGCAACCTCATCTCAGCTCGCCAACACCATTCAATCCACATCATAA
CGGAAAGACTGAGCCTGATAAGCGAAGTCTTTATCAACATGCTGAACACTATGGGCAAAGTAAGGTAGCTAAATCTGCTT
GGCAATCATTTGGTTTGGCAGCATTTGCAGGCGGTTTTATTGCCTTAGCTTTTGTATTTTATATCACAGTAACAACGGGG
GCGTCTGGGAGTGCTTGGGGGCTTGTACGCTTAGCGGGAGGGTTAGCTTTTAGTTTGGGACTGATGTTGGTGGTGATTTG
TGGCGGCGAGCTGTTCACGAGTACCGTGTTGAGTTCTGTTGCTTGGGCACAAAAGAAAGTCACTACTAAACAACTTGTTA
TGTGCTGGACGCGGGTCTACCTGGGTAATCTACTCGGTGCAATGGTGATGTTGCTACTGATTATGTCTGCCCGTATGTAT
CTGCTCGACGGGGGACGCTGGGGGATAAATGCCCTAACGATCGCTCAGCATAAATTACATCACGGCTGGTTTCAGGCATT
TAGTTTAGGGATCCTTTGTAACATGTTGGTTTGTTTAGGGGTATGGATGACCTTTGCGAGCCGTGATGCGTTAACCAAAG
CGATATTACTGATGTTGCCAGTCGCCATGTTTGTGAGCAGTGGTTTTGAGCACAGCATAGCTAATCTGTTTATGGTGCCC
CTAGGAATTGCCATTCATCTTTTTGCTGATCAGACATTTTTTACCTCAGCAGCTATTGATGCCGCGCAATTCAGTGATTT
AACCCTCAGCCACTTTGTCATCAAGAATCTTATTCCGGTGACACTGGGCAATATTGTTGGTGGTGGCATTTTTGTGGGAC
TCGGTTATTGGTGGATTGAGCAAGCCAAACCTCAACACCCATCAAACATTGAGCGCCACACCCCAACATTAACCTTAGTC
AATGCATTGACTGCGACTTCTTCTTACTTAAACAACGATATGCCAACGGATACCGCGCTTGCTAATGAGCCTGCGTTATC
AATAAGTCCTGATGCTCATATTTATTCTATTTCTGCCGGAGATCAACCTACTATGTTAAATGCCAAAGCGGTTTCAAAAC
CTATTCCTAAAATGTGTGTGAGTGATGTGATGGACAGACATCCCATCACGCTAAGTGCCGATCAATCTGTCTATGCAGGT
TTAAAATTATTAAGTGATGCAAATGTCCGCTCTGCGCCTGTGGTCGATGATAAACAAGCATTAGTGGGGTTTATTTCACA
ACAAGATCTATTACGAAGTCTTTGGGCACAGGAGTTTACCCGAGGTGTACCTGATAAAGTGGCTGACTTAATGCAAACCG
AGGTGTTAACTGTCTCTCCTAAGGATCCACTGGATGCATTAATTGAGTTAATGGTTGTTGATCGTCAAAAGTTGTTTCCT
GTAACGGATAGCGGACTGATGATTGCGAGTACTTATCAAAGTTATGAAGAACGGTTGCGCCATGCCTATGCCACTAAGCC
AAGTGCTTTTCCGGTGATAGAGCAGGGTAAGTTGTGTGGGATGGTGACTCGAGAAGAGATTGCCAAAGCGGTTTGTGGTG
CTTTTTACGATTAA

Upstream 100 bases:

>100_bases
TTGTTTGATTGCACTATTCACAGGGCAATGAAATTACTGGAGAAATTTATGTTGTTAACGCTATTTTCCCCGAAAATATT
CAGTGTTGGAGCTAACTGAG

Downstream 100 bases:

>100_bases
GTTTGATCGCTGTTAATTTAACCCCGAAACAAGCTGGGTTGTTTCGGGTTTTTCATTTTCTTGCTTCGGTATAAAGGTAT
TAGCGTTTAATTGTAATTTG

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKASHYDAHMRAQPHLSSPTPFNPHHNGKTEPDKRSLYQHAEHYGQSKVAKSAWQSFGLAAFAGGFIALAFVFYITVTTG
ASGSAWGLVRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKKVTTKQLVMCWTRVYLGNLLGAMVMLLLIMSARMY
LLDGGRWGINALTIAQHKLHHGWFQAFSLGILCNMLVCLGVWMTFASRDALTKAILLMLPVAMFVSSGFEHSIANLFMVP
LGIAIHLFADQTFFTSAAIDAAQFSDLTLSHFVIKNLIPVTLGNIVGGGIFVGLGYWWIEQAKPQHPSNIERHTPTLTLV
NALTATSSYLNNDMPTDTALANEPALSISPDAHIYSISAGDQPTMLNAKAVSKPIPKMCVSDVMDRHPITLSADQSVYAG
LKLLSDANVRSAPVVDDKQALVGFISQQDLLRSLWAQEFTRGVPDKVADLMQTEVLTVSPKDPLDALIELMVVDRQKLFP
VTDSGLMIASTYQSYEERLRHAYATKPSAFPVIEQGKLCGMVTREEIAKAVCGAFYD

Sequences:

>Translated_537_residues
MKASHYDAHMRAQPHLSSPTPFNPHHNGKTEPDKRSLYQHAEHYGQSKVAKSAWQSFGLAAFAGGFIALAFVFYITVTTG
ASGSAWGLVRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKKVTTKQLVMCWTRVYLGNLLGAMVMLLLIMSARMY
LLDGGRWGINALTIAQHKLHHGWFQAFSLGILCNMLVCLGVWMTFASRDALTKAILLMLPVAMFVSSGFEHSIANLFMVP
LGIAIHLFADQTFFTSAAIDAAQFSDLTLSHFVIKNLIPVTLGNIVGGGIFVGLGYWWIEQAKPQHPSNIERHTPTLTLV
NALTATSSYLNNDMPTDTALANEPALSISPDAHIYSISAGDQPTMLNAKAVSKPIPKMCVSDVMDRHPITLSADQSVYAG
LKLLSDANVRSAPVVDDKQALVGFISQQDLLRSLWAQEFTRGVPDKVADLMQTEVLTVSPKDPLDALIELMVVDRQKLFP
VTDSGLMIASTYQSYEERLRHAYATKPSAFPVIEQGKLCGMVTREEIAKAVCGAFYD
>Mature_537_residues
MKASHYDAHMRAQPHLSSPTPFNPHHNGKTEPDKRSLYQHAEHYGQSKVAKSAWQSFGLAAFAGGFIALAFVFYITVTTG
ASGSAWGLVRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKKVTTKQLVMCWTRVYLGNLLGAMVMLLLIMSARMY
LLDGGRWGINALTIAQHKLHHGWFQAFSLGILCNMLVCLGVWMTFASRDALTKAILLMLPVAMFVSSGFEHSIANLFMVP
LGIAIHLFADQTFFTSAAIDAAQFSDLTLSHFVIKNLIPVTLGNIVGGGIFVGLGYWWIEQAKPQHPSNIERHTPTLTLV
NALTATSSYLNNDMPTDTALANEPALSISPDAHIYSISAGDQPTMLNAKAVSKPIPKMCVSDVMDRHPITLSADQSVYAG
LKLLSDANVRSAPVVDDKQALVGFISQQDLLRSLWAQEFTRGVPDKVADLMQTEVLTVSPKDPLDALIELMVVDRQKLFP
VTDSGLMIASTYQSYEERLRHAYATKPSAFPVIEQGKLCGMVTREEIAKAVCGAFYD

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=266, Percent_Identity=50.3759398496241, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788837, Length=256, Percent_Identity=40.234375, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87082249, Length=250, Percent_Identity=30, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=240, Percent_Identity=27.9166666666667, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 58500; Mature: 58500

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]