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The map label for this gene is fadI

Identifier: 120598366

GI number: 120598366

Start: 1758128

End: 1759438

Strand: Direct

Name: fadI

Synonym: Sputw3181_1548

Alternate gene names: 120598366

Gene position: 1758128-1759438 (Clockwise)

Preceding gene: 120598348

Following gene: 120598367

Centisome position: 37.34

GC content: 50.19

Gene sequence:

>1311_bases
ATGAGTGATAGACAACAGGTTACGAACGCCAAGGGTGAACGTATCGCCATTGTAGCGGGTTTAAGAACGCCATTTGCGAA
ACAAGCCACGGCATTCCACGGTGTATCGGCATTAGATATGGGCAAAATGGTGGTCAATGAATTATTGGCGCGCTCTGAAC
TCGATCCTAAGCTGATTGAGCAATTAGTCTATGGACAAGTCGTGCAAATGCCGGCTGCACCGAATATAGCCCGGGAAATC
GTGCTCGGTACTGGCATGAATGTGTCGACTGATGCCTATAGTGTCACTCGCGCCTGTGCGACTAGCTTCCAATCTACTGT
CAACGTGGCCGAATCTATTATGACGGGCAATATCGACATCGGTATTGCGGGCGGCGCCGACTCTTCCTCTGTATTGCCTA
TCGGTGTGTCGAAAAAATTAGCTCATGCGTTAGTCGATTTGAATAAAGCCCGTAGTTTTGGGCAAAAGTTACAAATTTTC
CGCCGTTTAGGTTTAAAGGATTTATTACCTGTGCCGCCTGCAGTGGCAGAGTACTCAACGGGCTTGTCTATGGGACAAAC
CGCCGAGCAGATGGCAAAAACCTACAATATTAGCCGTGCCGATCAAGATGCCTTAGCGCACCGCTCACACACCTTAGCGA
GTGAAACTTGGGCTTCGGGCCATTTGCGTGACGAAGTGATGGTCGCGCATATACCGCCGTATAAGCAGTTTATCGATCGC
GATAACAATATCCGCGAAAACTCAGTACTGGAGTCATATGCTAAGTTGCGCCCTGCATTCGACAAACAACATGGTACTGT
GACGGCGGCCAATAGTACGCCACTGACGGACGGTGCGTCTGCGATTATCCTGATGAGTGAAGGTCGAGCCAAAGCCTTAG
GTTATCAACCGATTGGTTATATTAAGAGCTACGCCTTTAGCGCGATCGATGTGTGGCAAGATATGTTGATGGGGCCATCC
TACGCGACGCCACTGGCGCTGAAGCGTGCTGGAATGGAATTAGAAGATCTTACTCTGATTGAAATGCACGAAGCCTTTGC
GGCGCAGACATTAGCGAATATGCAGATGTTTGCTTCGAAAAAATTTGCCGAAGAAAAACTCGGCCGCAACCGCCCGATTG
GCGAAATCGACATGAGCAAGTTTAACGTGCTCGGTGGCTCGCTCGCTTATGGTCATCCTTTTGCGGCAACGGGAACCCGT
TTAATAACCCAAGTGTGTCGTGAGCTTAAGCGCCGTGGTGGTGGAACTGGCTTAACGACGGCCTGTGCGGCCGGTGGTTT
GGGCGTGGCTATGATAGTGGAAGTGGAGTAA

Upstream 100 bases:

>100_bases
GTTATTGGGCGTTTTTCGCCGTAAAACAATGGGTCAAACATGGAAATCGATAGCGCCGCCTGAAATCTCTCAGCGATAGC
GCACATAAAGAGGGTGACAA

Downstream 100 bases:

>100_bases
TTGGCATGGAAAAGACCTTTAATTTAACCCGCCGCGACGACGGCATCGCCATTTTGACTATGGATGTTCCTGGCGAAACC
ATGAACACCCTAAAAGCACA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIEQLVYGQVVQMPAAPNIAREI
VLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNIDIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIF
RRLGLKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHIPPYKQFIDR
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGYIKSYAFSAIDVWQDMLMGPS
YATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASKKFAEEKLGRNRPIGEIDMSKFNVLGGSLAYGHPFAATGTR
LITQVCRELKRRGGGTGLTTACAAGGLGVAMIVEVE

Sequences:

>Translated_436_residues
MSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIEQLVYGQVVQMPAAPNIAREI
VLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNIDIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIF
RRLGLKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHIPPYKQFIDR
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGYIKSYAFSAIDVWQDMLMGPS
YATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASKKFAEEKLGRNRPIGEIDMSKFNVLGGSLAYGHPFAATGTR
LITQVCRELKRRGGGTGLTTACAAGGLGVAMIVEVE
>Mature_435_residues
SDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIEQLVYGQVVQMPAAPNIAREIV
LGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNIDIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFR
RLGLKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHIPPYKQFIDRD
NNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGYIKSYAFSAIDVWQDMLMGPSY
ATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASKKFAEEKLGRNRPIGEIDMSKFNVLGGSLAYGHPFAATGTRL
ITQVCRELKRRGGGTGLTTACAAGGLGVAMIVEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=41.4285714285714, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI167614485, Length=423, Percent_Identity=31.9148936170213, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI4557237, Length=434, Percent_Identity=27.6497695852535, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI148539872, Length=431, Percent_Identity=29.4663573085847, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=29.0697674418605, Blast_Score=172, Evalue=7e-43,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=73.0046948356808, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=427, Percent_Identity=32.7868852459016, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=34.0425531914894, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI48994986, Length=433, Percent_Identity=33.256351039261, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1787663, Length=424, Percent_Identity=31.3679245283019, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=40.855106888361, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=31.4553990610329, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=422, Percent_Identity=28.1990521327014, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17535921, Length=426, Percent_Identity=27.4647887323944, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17535917, Length=430, Percent_Identity=23.0232558139535, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325229, Length=430, Percent_Identity=29.3023255813954, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322031, Length=432, Percent_Identity=30.0925925925926, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI17137578, Length=434, Percent_Identity=42.3963133640553, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24655093, Length=424, Percent_Identity=29.7169811320755, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17648125, Length=429, Percent_Identity=29.1375291375291, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24640423, Length=430, Percent_Identity=24.6511627906977, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46806; Mature: 46675

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIE
CCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QLVYGQVVQMPAAPNIAREIVLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNIDI
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFRRLGLKDLLPVPPAVAEYST
EEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHC
GLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHIPPYKQFIDR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGY
CCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCHHCCCCCHHH
IKSYAFSAIDVWQDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFAEEKLGRNRPIGEIDMSKFNVLGGSLAYGHPFAATGTRLITQVCRELKRRGGGTGLTT
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGVAMIVEVE
HHHCCCCCEEEEEEEC
>Mature Secondary Structure 
SDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIE
CCHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QLVYGQVVQMPAAPNIAREIVLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNIDI
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFRRLGLKDLLPVPPAVAEYST
EEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHC
GLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHIPPYKQFIDR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGY
CCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCHHCCCCCHHH
IKSYAFSAIDVWQDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFAEEKLGRNRPIGEIDMSKFNVLGGSLAYGHPFAATGTRLITQVCRELKRRGGGTGLTT
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGVAMIVEVE
HHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA