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The map label for this gene is fliA

Identifier: 120598267

GI number: 120598267

Start: 1631110

End: 1631823

Strand: Direct

Name: fliA

Synonym: Sputw3181_1444

Alternate gene names: 120598267

Gene position: 1631110-1631823 (Clockwise)

Preceding gene: 120598266

Following gene: 120598268

Centisome position: 34.64

GC content: 43.28

Gene sequence:

>714_bases
GTGAATAAAGCCGCAGCGTATACTTGTTTAGACAATAAATCGTCTATCGTTGAACAGTATGCTCCGTTGGTTAAAAGAAT
TGCACATCATTTACTCGCCAGACTGCCAGCTTCTGTGCAGCTAGATGATCTACTACAAGCTGGGATGATGGGACTATTAG
AAGCATCATCCAAGTTTGATGGTGGCAAAGGTGCAAAATTTGAAACCTTCGCTGGCATACGGATCCGTGGTGCCATGCTG
GACGAAATTCGTCGAGGTGATTGGGTCCCACGTTCTGTTCATCGTAATAATCGTAGGGTAGCACAAGCCATTGATGAGTT
AGAAAAAGAACTGGGCCGTGATGCACGTGACACAGAAATTGCAGAAAAACTTGATATGACGCTCGATGAGTACCATCATA
TTCTTAACGATGTTTCTGTCGGAAAAATCATAGGGATAGAAGACTTAGGTGTATCGCAAGATATACTAACGCTGAGCGAT
AGGTCCGACGATGGCTCGTTTGAAGCTCTCGCTGAGACTCAGTTTCATTCCGCATTAGTTGAGGCAATAAAATTATTGCC
AGAAAGAGATGCGTTAGTGTTATCGCTGTACTACGATGAAGCATTGAATTTAAAAGAGATTGGCGCCATTCTTGAAGTAA
GCGAATCGCGAGTTAGCCAGATATTAAGTCAGGCTATGTTGCGACTCAAAGCTAAACTCAAGCACTGGACATAA

Upstream 100 bases:

>100_bases
AAAATCATGAGTTGGCCTGTACCGCAACAACCTGGTGGGCATTTGGAGTTTTTCGTTGAACGTTTAGTCCAACGACCAGA
TTTTCAAGAGGAAAAAACGA

Downstream 100 bases:

>100_bases
GTATAATTATTAATCGAGCACATAATGTCAGCTCACCGGAGGAAACCTTGGACAAGAATATGAAGATTCTCATTGTTGAC
GACTTTTCAACAATGAGACG

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNKAAAYTCLDNKSSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEKELGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTLSD
RSDDGSFEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKAKLKHWT

Sequences:

>Translated_237_residues
MNKAAAYTCLDNKSSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEKELGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTLSD
RSDDGSFEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKAKLKHWT
>Mature_237_residues
MNKAAAYTCLDNKSSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEKELGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTLSD
RSDDGSFEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKAKLKHWT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=237, Percent_Identity=49.3670886075949, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1789448, Length=236, Percent_Identity=28.3898305084746, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26422; Mature: 26422

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAAYTCLDNKSSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFD
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GGKGAKFETFAGIRIRGAMLDEIRRGDWVPRSVHRNNRRVAQAIDELEKELGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
AEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTLSDRSDDGSFEALAETQFHSALV
HHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHH
EAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKAKLKHWT
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKAAAYTCLDNKSSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFD
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GGKGAKFETFAGIRIRGAMLDEIRRGDWVPRSVHRNNRRVAQAIDELEKELGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
AEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTLSDRSDDGSFEALAETQFHSALV
HHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHH
EAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKAKLKHWT
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]