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The map label for this gene is flhF

Identifier: 120598265

GI number: 120598265

Start: 1628849

End: 1630231

Strand: Direct

Name: flhF

Synonym: Sputw3181_1442

Alternate gene names: 120598265

Gene position: 1628849-1630231 (Clockwise)

Preceding gene: 120598264

Following gene: 120598266

Centisome position: 34.59

GC content: 46.06

Gene sequence:

>1383_bases
GTGAAGATTAAACGATTTTTTGCCAAAGACATGCGTGCCGCACTGGCCCAAGTGAAAGACACTCTCGGCTCAGATGCCGT
TATCATGTCAAACAAAAAGGTAAATGGCGGTATCGAAATTGTCGCCGCGGTTGACTACGATGAGCCAAAAGCGAAGGCGT
CCGCGACACCTCCTGCACCTACTTTTATGGATGTCAGTGATGACTTAGTGTCTCTTGGTGGAAAGCAGCCTGTGCGTGCA
CAGAGCAGTGTCAAACCATCGGCGCCAGCAGATTCATTACAAGCTTTGCTCGAGAAACAACAAAGCCGTTTAAATCAGCA
GTTATCGCATCAGCAACCTGAGGCCGATTTACCCGTATGGGCAAAAGGGTTACAGGCACCAGTAGCATCGAAGTCTGAGC
GCCGAGACATTCCGGCCGCTTTTGATAAAAAGCCGCAAACCACACAAAAACAAAATGCTGATATTGAAGCCATGCGTGAT
GAATTAGCATCACTACGTAATTTACTGACTCACCAAGTGTCTTCTTTGATGACAGAGCATAAAAAACGTATCGATCCCGT
TGGCGCTATGCTGGAATCTAAGTTACTTGAAGCCGAATTTTCTCCTGCAGTTGCTGCGAAATTAGCCGCGTTGAGTCAGC
ATTATACCCCTGCTGAGTTAGTGCGAGCACTACCCCAAAGTCTAGCTAATATGCTTGATAATCAAGGTGATGATATAGTT
AGGCAGGGTGGAGTAGTGGCTTTAGTTGGCCCTACTGGTGTGGGTAAAACGACCTCATTAGCTAAATTGGCTGCGCGTTT
TGCTGCTCACCATGGGCCGGAACAAGTCGCATTAATTACCACAGATCATTATCGCATTGGCGCCTATGAGCAATTAGCGA
CCTATGGCAAAATAATGGGCTGTCCAGTTAAGCAAGCCCATGATCTCAATGAATTAGAACAAATTCTTTATCAGTTCAGA
AATCGCAAGCTAGTATTGATAGATACCGCTGGTATGGGGCAACGAGATATGCGGCTTTACCAGCAACTTGATAATTTAAC
TGCAAATAGCAGAATACCTATTCGCAGTTATCTGGTACTGTCTGCCACAGGACAGCGCCGAGTGTTGCAAGATGCTGTTA
ATCATTTTAAACGCATTCCTTTATCTGGTGCCGTGCTCACAAAACTCGATGAATCAGTCTCCCTCGCGGGTGCGCTGAGT
GTGTTGATCCAAAGTGGGCTACCATTAAGTTATGTTACTGATGGACAAAGGGTTCCAGAAGATATGAAAGTCGCAGACAC
CCTAATGTTAGCGCAGCAGGCGCTTGCGACATTAGATAGTACAGAACAACAATCATTACAAGACACAGCGTGGTCAGATA
ATATGGCCTGTGCATTTGAGTAG

Upstream 100 bases:

>100_bases
AACATACTATTCCTAATCTCAGAGTGATTTCTTACCAAGAAATCCCCGATGAAAAGCAAATACGCATTGTATCTGCTGTC
GGCCAGTAGAGGACGCATAA

Downstream 100 bases:

>100_bases
AGTTATGACCCTGGATCAAGCAAGTGGTTTACGTATGATGAATCAACCCTATAACGAAAAAGTGAAAGTAATCGCTGTCA
CAGGTGGTAAAGGTGGCGTG

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKIKRFFAKDMRAALAQVKDTLGSDAVIMSNKKVNGGIEIVAAVDYDEPKAKASATPPAPTFMDVSDDLVSLGGKQPVRA
QSSVKPSAPADSLQALLEKQQSRLNQQLSHQQPEADLPVWAKGLQAPVASKSERRDIPAAFDKKPQTTQKQNADIEAMRD
ELASLRNLLTHQVSSLMTEHKKRIDPVGAMLESKLLEAEFSPAVAAKLAALSQHYTPAELVRALPQSLANMLDNQGDDIV
RQGGVVALVGPTGVGKTTSLAKLAARFAAHHGPEQVALITTDHYRIGAYEQLATYGKIMGCPVKQAHDLNELEQILYQFR
NRKLVLIDTAGMGQRDMRLYQQLDNLTANSRIPIRSYLVLSATGQRRVLQDAVNHFKRIPLSGAVLTKLDESVSLAGALS
VLIQSGLPLSYVTDGQRVPEDMKVADTLMLAQQALATLDSTEQQSLQDTAWSDNMACAFE

Sequences:

>Translated_460_residues
MKIKRFFAKDMRAALAQVKDTLGSDAVIMSNKKVNGGIEIVAAVDYDEPKAKASATPPAPTFMDVSDDLVSLGGKQPVRA
QSSVKPSAPADSLQALLEKQQSRLNQQLSHQQPEADLPVWAKGLQAPVASKSERRDIPAAFDKKPQTTQKQNADIEAMRD
ELASLRNLLTHQVSSLMTEHKKRIDPVGAMLESKLLEAEFSPAVAAKLAALSQHYTPAELVRALPQSLANMLDNQGDDIV
RQGGVVALVGPTGVGKTTSLAKLAARFAAHHGPEQVALITTDHYRIGAYEQLATYGKIMGCPVKQAHDLNELEQILYQFR
NRKLVLIDTAGMGQRDMRLYQQLDNLTANSRIPIRSYLVLSATGQRRVLQDAVNHFKRIPLSGAVLTKLDESVSLAGALS
VLIQSGLPLSYVTDGQRVPEDMKVADTLMLAQQALATLDSTEQQSLQDTAWSDNMACAFE
>Mature_460_residues
MKIKRFFAKDMRAALAQVKDTLGSDAVIMSNKKVNGGIEIVAAVDYDEPKAKASATPPAPTFMDVSDDLVSLGGKQPVRA
QSSVKPSAPADSLQALLEKQQSRLNQQLSHQQPEADLPVWAKGLQAPVASKSERRDIPAAFDKKPQTTQKQNADIEAMRD
ELASLRNLLTHQVSSLMTEHKKRIDPVGAMLESKLLEAEFSPAVAAKLAALSQHYTPAELVRALPQSLANMLDNQGDDIV
RQGGVVALVGPTGVGKTTSLAKLAARFAAHHGPEQVALITTDHYRIGAYEQLATYGKIMGCPVKQAHDLNELEQILYQFR
NRKLVLIDTAGMGQRDMRLYQQLDNLTANSRIPIRSYLVLSATGQRRVLQDAVNHFKRIPLSGAVLTKLDESVSLAGALS
VLIQSGLPLSYVTDGQRVPEDMKVADTLMLAQQALATLDSTEQQSLQDTAWSDNMACAFE

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1789874, Length=191, Percent_Identity=31.413612565445, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 50020; Mature: 50020

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRFFAKDMRAALAQVKDTLGSDAVIMSNKKVNGGIEIVAAVDYDEPKAKASATPPAP
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCC
TFMDVSDDLVSLGGKQPVRAQSSVKPSAPADSLQALLEKQQSRLNQQLSHQQPEADLPVW
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AKGLQAPVASKSERRDIPAAFDKKPQTTQKQNADIEAMRDELASLRNLLTHQVSSLMTEH
HHHHCCCCCCCHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKRIDPVGAMLESKLLEAEFSPAVAAKLAALSQHYTPAELVRALPQSLANMLDNQGDDIV
HHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHH
RQGGVVALVGPTGVGKTTSLAKLAARFAAHHGPEQVALITTDHYRIGAYEQLATYGKIMG
HCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCEECCHHHHHHHHHHHHC
CPVKQAHDLNELEQILYQFRNRKLVLIDTAGMGQRDMRLYQQLDNLTANSRIPIRSYLVL
CCCHHHCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHEEEE
SATGQRRVLQDAVNHFKRIPLSGAVLTKLDESVSLAGALSVLIQSGLPLSYVTDGQRVPE
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
DMKVADTLMLAQQALATLDSTEQQSLQDTAWSDNMACAFE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MKIKRFFAKDMRAALAQVKDTLGSDAVIMSNKKVNGGIEIVAAVDYDEPKAKASATPPAP
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCC
TFMDVSDDLVSLGGKQPVRAQSSVKPSAPADSLQALLEKQQSRLNQQLSHQQPEADLPVW
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AKGLQAPVASKSERRDIPAAFDKKPQTTQKQNADIEAMRDELASLRNLLTHQVSSLMTEH
HHHHCCCCCCCHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKRIDPVGAMLESKLLEAEFSPAVAAKLAALSQHYTPAELVRALPQSLANMLDNQGDDIV
HHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHH
RQGGVVALVGPTGVGKTTSLAKLAARFAAHHGPEQVALITTDHYRIGAYEQLATYGKIMG
HCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCEECCHHHHHHHHHHHHC
CPVKQAHDLNELEQILYQFRNRKLVLIDTAGMGQRDMRLYQQLDNLTANSRIPIRSYLVL
CCCHHHCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHEEEE
SATGQRRVLQDAVNHFKRIPLSGAVLTKLDESVSLAGALSVLIQSGLPLSYVTDGQRVPE
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
DMKVADTLMLAQQALATLDSTEQQSLQDTAWSDNMACAFE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9503621 [H]