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The map label for this gene is rlmN [H]

Identifier: 120598175

GI number: 120598175

Start: 1531325

End: 1532524

Strand: Direct

Name: rlmN [H]

Synonym: Sputw3181_1352

Alternate gene names: 120598175

Gene position: 1531325-1532524 (Clockwise)

Preceding gene: 120598172

Following gene: 120598176

Centisome position: 32.52

GC content: 43.67

Gene sequence:

>1200_bases
TTGTGTCGCTGGCTAAAAATTTATTGCATCTGTATAATTCGCCGCCCTGTTGCAGGTTTTGGTGTTGAGGTGAGTGGTAT
GAGTGAAAAAAAGATCAATTTATTAGACCTTGATCGTAAGGCTATGCGCGCATTATTTGCGGATCTAGGGGAAAAGCCCT
TTCGTGCCGATCAACTCATGAAGTGGATTTATCATTTCGGTGTCAGCGACTTCGAAGAAATGACGAATATCAATAAAGTG
TTGCGTCAAAAATTGGCTGCACGCTGTGAAATTGTAGCGCCTGAAATTTCCAGTTTCCAAAAGTCCACCGACGGGACAAT
TAAGTTTGCGATACATGTTGGTGAAGGGCAGGAAGTTGAAACTGTCTATATTCCAGAAGATGACCGTGCGACTTTGTGTG
TATCATCTCAGGTGGGATGTGCGCTTGAATGTACGTTTTGCTCAACGGCTCAACAAGGTTTTAACCGTAATTTGACCGTA
TCTGAAATTGTCGGTCAAATCTGGCGGGTTTCACATTTCTTAGGTTTTGCAAAGGATACTGGCGATCGTCCGATCACCAA
TGTGGTGATGATGGGTATGGGTGAGCCACTGCTTAATTTAGCCAACGTTATCCCCGCAATGGATATTATGCTGGACGATT
TTGGGTTTAGCTTATCAAAACGCCGGGTAACCCTGTCAACTTCTGGTGTTGTGCCAGCCCTTGATAAACTTGGTGATGCC
CTTGATGTGGCATTGGCCGTGAGCATTCACGCTCCTAATGATGAGTTACGGGATATTTTAGTCCCAGTGAATAAAAAGTA
TCCATTGCAGGAGTTCCTTGCAGGGATCCGTCGTTATATTGCGAAATCAAATGCAAACCGTGGCCGTGTAACCGTTGAAT
ATGTGATGCTCGATCATATCAACGACAGCACTGAGCAAGCTCACGAGTTAGCAAAACTAATGAAAGATACGCCTTGTAAA
GTGAATTTAATTCCTTTTAATCCGTATCCTGGTTCACCCTATGGTCGTTCATCGAACTCGCGTATTGATCGTTTTTCGAA
AGTATTAATGGAATATGGCTTAACGGTGATAGTACGTAAGACCCGCGGTGACGATATTGATGCTGCCTGCGGTCAGTTAG
CGGGGGATATTCGCGATAGAACTAAGCGTTTGGCAAAAAAACGCATGCAAGAAAATCAGATTTCAGTCACAATGAATTAA

Upstream 100 bases:

>100_bases
CAGCGACAAGCACTATAATGATGAGGGTTAACATATAGGTGGTATTACAACTCCGTATTTTAGGCCAACATTAGCCCAGC
GAGATTCTATCATAGTAGAG

Downstream 100 bases:

>100_bases
CTCATTGTTTGCACAGATTTATAGGCTTGCCCATGAAGCATGGATTGTCAGGGTTTTCATTAGCAATCACCTTGTCATTA
GTCATGACAGGGTGTGTCAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MCRWLKIYCICIIRRPVAGFGVEVSGMSEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKV
LRQKLAARCEIVAPEISSFQKSTDGTIKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTV
SEIVGQIWRVSHFLGFAKDTGDRPITNVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDA
LDVALAVSIHAPNDELRDILVPVNKKYPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAKLMKDTPCK
VNLIPFNPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Sequences:

>Translated_399_residues
MCRWLKIYCICIIRRPVAGFGVEVSGMSEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKV
LRQKLAARCEIVAPEISSFQKSTDGTIKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTV
SEIVGQIWRVSHFLGFAKDTGDRPITNVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDA
LDVALAVSIHAPNDELRDILVPVNKKYPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAKLMKDTPCK
VNLIPFNPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN
>Mature_399_residues
MCRWLKIYCICIIRRPVAGFGVEVSGMSEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKV
LRQKLAARCEIVAPEISSFQKSTDGTIKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTV
SEIVGQIWRVSHFLGFAKDTGDRPITNVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDA
LDVALAVSIHAPNDELRDILVPVNKKYPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAKLMKDTPCK
VNLIPFNPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=75.6830601092896, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 44621; Mature: 44621

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRWLKIYCICIIRRPVAGFGVEVSGMSEKKINLLDLDRKAMRALFADLGEKPFRADQLM
CCCHHEEHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHH
KWIYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGTIKFAIHVGEGQEVE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCCCEEEEEEEECCCCEEE
TVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKDT
EEEECCCCCEEEEEECCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GDRPITNVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDA
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCH
LDVALAVSIHAPNDELRDILVPVNKKYPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHI
HEEEEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
NDSTEQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRK
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
TRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MCRWLKIYCICIIRRPVAGFGVEVSGMSEKKINLLDLDRKAMRALFADLGEKPFRADQLM
CCCHHEEHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHH
KWIYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGTIKFAIHVGEGQEVE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCCCEEEEEEEECCCCEEE
TVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKDT
EEEECCCCCEEEEEECCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GDRPITNVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDA
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCH
LDVALAVSIHAPNDELRDILVPVNKKYPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHI
HEEEEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
NDSTEQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRK
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
TRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA