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The map label for this gene is phrA [H]

Identifier: 120598121

GI number: 120598121

Start: 1482351

End: 1483832

Strand: Direct

Name: phrA [H]

Synonym: Sputw3181_1298

Alternate gene names: 120598121

Gene position: 1482351-1483832 (Clockwise)

Preceding gene: 120598120

Following gene: 120598122

Centisome position: 31.48

GC content: 44.53

Gene sequence:

>1482_bases
GTGATATCACTTAAAGAACAAATGGCTACGTCAAATGTAGGCGATACACTGATGTGGTTTCGTCAAGATTTAAGACTTGC
CGATAATCAAGCATTAACAGCGGCTTGCGATTGGGCAAGGGCGCAAGGTACTGCGCTTAGGGCTATATATATTGCAACTC
CCATCCAGTGGGCATGCCATGATGTAGCGCCTATTCAATTAGATTTTATTGAACGTCATATTAATCTGCTTGCGAAAGGT
CTTGGATGTTTAGGTATTCAGTTTGAGTTAATTCAATTAGAGCGTTTTGCGGATATCCCACAATTTATGCTTGATTATTG
TCAGCAGCACAATATTACAAGGGTGTTTGCTGGCTCAGAGCCAGAGATAAACGAACGAGTTCGCGATCAGGCATGCATTG
ATGCGGGTGTGCCGCTGTTGTTAACTGATGAACATTGTTTGCTCGCGCCAGGCACAGTGTTGAACTTATCGGGTGAAATG
TATCGAGTTTTTACCCCTTTTAGTCGAAAGTGGCGAGAAATTGCCGCCCGTCATGCCATTTTGCCACTCGGAGTTCCCGC
ACCACTTGGGCCCGTTTTACCAGAACCTAAAGCGTTAAATTTAGCCGTGAATAAAGTATCGAGCGAACAATGGGGCGCGG
GAGAAGGGCAAGCTAAGCGTTTGCTGTGCGATTTTGTACAGCAAAAAGTGCAAGATTATAAACAGGATCGTGATTTTCCG
GCCCTAGATGGGACGAGTTGTATTTCTCCTTATTTGGCGATTGGCGTGCTCAGTGCACGCCAATGTGTTGCCGCATTATT
ACAGCGTTTTCCTGAGGTGATCGTGGATGATACAAGCCTTGGCCGAACTTGGCTTAATGAGCTTGTCTGGCGTGAGTTTT
ATCGTCATTTATTAGTCGCATTTCCAGACTTATCTAAAAATTATAATTTTAATCGTCAAGCAGACCAAGTTCAGTGGCGT
AATAATATTGAAGAGTTTAAGGCTTGGTGCGAGGGACGAACTGGTTATCCTATTGTTGATGCAGCTATGCGTCAGCTTAA
TCAAACAGGTTGGATGCACAATCGCCTGCGTATGGTGGTCGCCAGTTTCTTAACCAAACATTTGTTAATTGATTGGCGTT
GGGGTGAGCGCTATTTCCGCCAAAAACTCATTGATGGTGACTTAGCGGCTAATAATGGTGGCTGGCAATGGTCTGCTGGA
TGTGGATGTGATGCCCAACCTTATTTCCGTATTTTTAATCCAATGAGCCAGAGTGAAAAGTTTGATCCAGATGGCAGCTT
TATCCGTAAATACTTACCAGAGCTGGCATCTTGGGGAATAAAGCAACTGCATCAACCCAATACCGCTAAGACTCCTTTAT
TGTTTAAACAGCCATTATTTGCCGAACAAGCCGCTTATCCGAGTGCCATAGTGGATCACACCGAAGCAAGGGTTAGAGCA
TTAAGTGTGCTCGGCGTATTGAAAAAGGCTTCTGCCTTATAA

Upstream 100 bases:

>100_bases
GTGTCACCTAAATCGCAGGCTTCGACTCGGCATCAAACGGTCAATCTGCAGCAGGGTGTATAAGTAAAAGGCGGTATTAA
AAATATGAGTGGAGATAACA

Downstream 100 bases:

>100_bases
ATTTGGGTTCTGGAGTAATACAGCGATGACTGAAGTAAATCCAAATACACTTGTTGACGATTTTATCCAGCTATACCAAG
CATTAAATAAAGACAATCTG

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MISLKEQMATSNVGDTLMWFRQDLRLADNQALTAACDWARAQGTALRAIYIATPIQWACHDVAPIQLDFIERHINLLAKG
LGCLGIQFELIQLERFADIPQFMLDYCQQHNITRVFAGSEPEINERVRDQACIDAGVPLLLTDEHCLLAPGTVLNLSGEM
YRVFTPFSRKWREIAARHAILPLGVPAPLGPVLPEPKALNLAVNKVSSEQWGAGEGQAKRLLCDFVQQKVQDYKQDRDFP
ALDGTSCISPYLAIGVLSARQCVAALLQRFPEVIVDDTSLGRTWLNELVWREFYRHLLVAFPDLSKNYNFNRQADQVQWR
NNIEEFKAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDLAANNGGWQWSAG
CGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPNTAKTPLLFKQPLFAEQAAYPSAIVDHTEARVRA
LSVLGVLKKASAL

Sequences:

>Translated_493_residues
MISLKEQMATSNVGDTLMWFRQDLRLADNQALTAACDWARAQGTALRAIYIATPIQWACHDVAPIQLDFIERHINLLAKG
LGCLGIQFELIQLERFADIPQFMLDYCQQHNITRVFAGSEPEINERVRDQACIDAGVPLLLTDEHCLLAPGTVLNLSGEM
YRVFTPFSRKWREIAARHAILPLGVPAPLGPVLPEPKALNLAVNKVSSEQWGAGEGQAKRLLCDFVQQKVQDYKQDRDFP
ALDGTSCISPYLAIGVLSARQCVAALLQRFPEVIVDDTSLGRTWLNELVWREFYRHLLVAFPDLSKNYNFNRQADQVQWR
NNIEEFKAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDLAANNGGWQWSAG
CGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPNTAKTPLLFKQPLFAEQAAYPSAIVDHTEARVRA
LSVLGVLKKASAL
>Mature_493_residues
MISLKEQMATSNVGDTLMWFRQDLRLADNQALTAACDWARAQGTALRAIYIATPIQWACHDVAPIQLDFIERHINLLAKG
LGCLGIQFELIQLERFADIPQFMLDYCQQHNITRVFAGSEPEINERVRDQACIDAGVPLLLTDEHCLLAPGTVLNLSGEM
YRVFTPFSRKWREIAARHAILPLGVPAPLGPVLPEPKALNLAVNKVSSEQWGAGEGQAKRLLCDFVQQKVQDYKQDRDFP
ALDGTSCISPYLAIGVLSARQCVAALLQRFPEVIVDDTSLGRTWLNELVWREFYRHLLVAFPDLSKNYNFNRQADQVQWR
NNIEEFKAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDLAANNGGWQWSAG
CGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPNTAKTPLLFKQPLFAEQAAYPSAIVDHTEARVRA
LSVLGVLKKASAL

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=508, Percent_Identity=26.9685039370079, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI4758072, Length=485, Percent_Identity=27.6288659793814, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI188536103, Length=301, Percent_Identity=30.5647840531561, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1786926, Length=485, Percent_Identity=45.5670103092784, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324962, Length=520, Percent_Identity=32.3076923076923, Blast_Score=249, Evalue=7e-67,
Organism=Drosophila melanogaster, GI17137248, Length=523, Percent_Identity=27.5334608030593, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24585455, Length=523, Percent_Identity=27.5334608030593, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24648152, Length=542, Percent_Identity=25.0922509225092, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55888; Mature: 55888

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLKEQMATSNVGDTLMWFRQDLRLADNQALTAACDWARAQGTALRAIYIATPIQWACH
CCCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHC
DVAPIQLDFIERHINLLAKGLGCLGIQFELIQLERFADIPQFMLDYCQQHNITRVFAGSE
CCCCHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCC
PEINERVRDQACIDAGVPLLLTDEHCLLAPGTVLNLSGEMYRVFTPFSRKWREIAARHAI
CCHHHHHHHHHHHHCCCCEEEECCCEEECCCCEEECCCCEEEEECHHHHHHHHHHHHHCC
LPLGVPAPLGPVLPEPKALNLAVNKVSSEQWGAGEGQAKRLLCDFVQQKVQDYKQDRDFP
EECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
ALDGTSCISPYLAIGVLSARQCVAALLQRFPEVIVDDTSLGRTWLNELVWREFYRHLLVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHH
FPDLSKNYNFNRQADQVQWRNNIEEFKAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVV
CCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHH
ASFLTKHLLIDWRWGERYFRQKLIDGDLAANNGGWQWSAGCGCDAQPYFRIFNPMSQSEK
HHHHHHHHEEEEHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHCCCCCCCC
FDPDGSFIRKYLPELASWGIKQLHQPNTAKTPLLFKQPLFAEQAAYPSAIVDHTEARVRA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHCCCHHHHHHHHHHHHH
LSVLGVLKKASAL
HHHHHHHHHHCCC
>Mature Secondary Structure
MISLKEQMATSNVGDTLMWFRQDLRLADNQALTAACDWARAQGTALRAIYIATPIQWACH
CCCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHC
DVAPIQLDFIERHINLLAKGLGCLGIQFELIQLERFADIPQFMLDYCQQHNITRVFAGSE
CCCCHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCC
PEINERVRDQACIDAGVPLLLTDEHCLLAPGTVLNLSGEMYRVFTPFSRKWREIAARHAI
CCHHHHHHHHHHHHCCCCEEEECCCEEECCCCEEECCCCEEEEECHHHHHHHHHHHHHCC
LPLGVPAPLGPVLPEPKALNLAVNKVSSEQWGAGEGQAKRLLCDFVQQKVQDYKQDRDFP
EECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
ALDGTSCISPYLAIGVLSARQCVAALLQRFPEVIVDDTSLGRTWLNELVWREFYRHLLVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHH
FPDLSKNYNFNRQADQVQWRNNIEEFKAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVV
CCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHH
ASFLTKHLLIDWRWGERYFRQKLIDGDLAANNGGWQWSAGCGCDAQPYFRIFNPMSQSEK
HHHHHHHHEEEEHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHCCCCCCCC
FDPDGSFIRKYLPELASWGIKQLHQPNTAKTPLLFKQPLFAEQAAYPSAIVDHTEARVRA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHCCCHHHHHHHHHHHHH
LSVLGVLKKASAL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]