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The map label for this gene is nemA [H]

Identifier: 120598115

GI number: 120598115

Start: 1474400

End: 1475467

Strand: Direct

Name: nemA [H]

Synonym: Sputw3181_1292

Alternate gene names: 120598115

Gene position: 1474400-1475467 (Clockwise)

Preceding gene: 120598113

Following gene: 120598117

Centisome position: 31.31

GC content: 46.91

Gene sequence:

>1068_bases
ATGAGCATACAATCTTCACCCAATCGATTGGGAAACCTGTTTGAAACCTACCAGCTTAATGAAACCATTACTTTAAATAA
CCGCATCTTAATGGCGCCGTTAACCCGTTGTATGGCAGATGCTGAACTTGTGCCTACCGCCGAAATGGTCGCTTACTATG
CTCGCCGAGCTGATGCAGGATTGATCATCACCGAAGCCACTATTATCCGCCCAGATGGTCAAGGCTATCCCAATACTCCA
GGTATTTTTACCCAGTCGCAAATTGCCGGTTGGCGCAAAGTGACCGATGCGGTACATGCCAAGGGCGGTAAAATTTTTGT
GCAGCTATGGCACACGGGTCGAGTAGCGCATCCGCACTTTTTTGTTGGCGGAGATGTACTTGCCCCTTCTGCACAGAAGA
TTGAAGGCTCTGTGCCAAGAATGCGTGAGCTAACCTATGTCACACCAAAACCAGCGACACTTGAAGATATTCAAGGATTA
GTACGCGATTACGCAAAAGCCGCTGAAAATGCTATCGAAGCAGGATTTGATGGTGTGGAAATTCATGGTGCCAATGGCTA
CTTGATTGATGCTTTCTTACACCATGACAGCAACCGTCGCACGGATGAATATGGTGGCACACCTGAAAAAATGTCGCGTT
TTGCGCTTGAAGTCGTAGACGCGATCATTGCACGCATTGGTAAGGACAGAACGGGGTTACGCCTTTCTCCGGGAGCCTAT
TTCAATATGGCGACCGATAGCCGCGATCGCGCCGTCTTCGACTACTTACTGCCAGAATTAGAAACGCGTGATATCGCTTT
TGTGCATATTGGGATCTTTGATGACAGTATGGAATTTGACTATTTAGGTGGCCGAGCTTCTAGTTATGTGCGAGCACATT
ATGGAAAAACTTTAGTAGGAGTAGGGAGTTATAGTGCTGAAACAGCAAGTGAGGCAATTGCGGCAGACAAGTTTGATTTG
ATTGCTATTGGTCGCCCCTTTATTGCAAACCCTGACTATATCGCTCGTGTTCGTGATGGGCTTGAACTTGTCCCTTACAG
CGATGAAATGCTGGCAACATTAGTATAA

Upstream 100 bases:

>100_bases
ACGGATTCTACCTAGTTTTGAACAAACATTCAAAAATGAACTTGAATGTTCATTCAAATAATAATATCTTGAATGCACAT
TCAAGAATAAGGCCGAAATA

Downstream 100 bases:

>100_bases
ATTTTGTCCGTGAGAGTATTTAAAGACAAAGGGTTAGCCATTGGCCAACCCTTTTTTATCACTTCATTCTCGGCTTAAAA
AGCTTAGACTTTAATCGGCT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSIQSSPNRLGNLFETYQLNETITLNNRILMAPLTRCMADAELVPTAEMVAYYARRADAGLIITEATIIRPDGQGYPNTP
GIFTQSQIAGWRKVTDAVHAKGGKIFVQLWHTGRVAHPHFFVGGDVLAPSAQKIEGSVPRMRELTYVTPKPATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPEKMSRFALEVVDAIIARIGKDRTGLRLSPGAY
FNMATDSRDRAVFDYLLPELETRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAADKFDL
IAIGRPFIANPDYIARVRDGLELVPYSDEMLATLV

Sequences:

>Translated_355_residues
MSIQSSPNRLGNLFETYQLNETITLNNRILMAPLTRCMADAELVPTAEMVAYYARRADAGLIITEATIIRPDGQGYPNTP
GIFTQSQIAGWRKVTDAVHAKGGKIFVQLWHTGRVAHPHFFVGGDVLAPSAQKIEGSVPRMRELTYVTPKPATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPEKMSRFALEVVDAIIARIGKDRTGLRLSPGAY
FNMATDSRDRAVFDYLLPELETRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAADKFDL
IAIGRPFIANPDYIARVRDGLELVPYSDEMLATLV
>Mature_354_residues
SIQSSPNRLGNLFETYQLNETITLNNRILMAPLTRCMADAELVPTAEMVAYYARRADAGLIITEATIIRPDGQGYPNTPG
IFTQSQIAGWRKVTDAVHAKGGKIFVQLWHTGRVAHPHFFVGGDVLAPSAQKIEGSVPRMRELTYVTPKPATLEDIQGLV
RDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPEKMSRFALEVVDAIIARIGKDRTGLRLSPGAYF
NMATDSRDRAVFDYLLPELETRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAADKFDLI
AIGRPFIANPDYIARVRDGLELVPYSDEMLATLV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=342, Percent_Identity=42.3976608187134, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789463, Length=343, Percent_Identity=30.9037900874636, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=376, Percent_Identity=26.8617021276596, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=345, Percent_Identity=27.536231884058, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=373, Percent_Identity=26.2734584450402, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=348, Percent_Identity=26.7241379310345, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=351, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=359, Percent_Identity=25.3481894150418, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=375, Percent_Identity=37.6, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321973, Length=366, Percent_Identity=36.6120218579235, Blast_Score=188, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39056; Mature: 38924

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQSSPNRLGNLFETYQLNETITLNNRILMAPLTRCMADAELVPTAEMVAYYARRADAG
CCCCCCCHHHHHHHHHEECCCEEEECCEEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
LIITEATIIRPDGQGYPNTPGIFTQSQIAGWRKVTDAVHAKGGKIFVQLWHTGRVAHPHF
EEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEE
FVGGDVLAPSAQKIEGSVPRMRELTYVTPKPATLEDIQGLVRDYAKAAENAIEAGFDGVE
EECCCEECCCHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDAFLHHDSNRRTDEYGGTPEKMSRFALEVVDAIIARIGKDRTGLRLSPGAY
EECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMATDSRDRAVFDYLLPELETRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVG
EEECCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCEEEE
VGSYSAETASEAIAADKFDLIAIGRPFIANPDYIARVRDGLELVPYSDEMLATLV
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEECCCCHHHHHHCC
>Mature Secondary Structure 
SIQSSPNRLGNLFETYQLNETITLNNRILMAPLTRCMADAELVPTAEMVAYYARRADAG
CCCCCCHHHHHHHHHEECCCEEEECCEEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
LIITEATIIRPDGQGYPNTPGIFTQSQIAGWRKVTDAVHAKGGKIFVQLWHTGRVAHPHF
EEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEE
FVGGDVLAPSAQKIEGSVPRMRELTYVTPKPATLEDIQGLVRDYAKAAENAIEAGFDGVE
EECCCEECCCHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDAFLHHDSNRRTDEYGGTPEKMSRFALEVVDAIIARIGKDRTGLRLSPGAY
EECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMATDSRDRAVFDYLLPELETRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVG
EEECCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCEEEE
VGSYSAETASEAIAADKFDLIAIGRPFIANPDYIARVRDGLELVPYSDEMLATLV
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]