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The map label for this gene is rpoS [H]

Identifier: 120598086

GI number: 120598086

Start: 1434923

End: 1435903

Strand: Direct

Name: rpoS [H]

Synonym: Sputw3181_1263

Alternate gene names: 120598086

Gene position: 1434923-1435903 (Clockwise)

Preceding gene: 120598085

Following gene: 120598088

Centisome position: 30.48

GC content: 41.08

Gene sequence:

>981_bases
ATGAGCCGTATAAATAGCACTGCCGCAGAAGAATTAGTCGATTTTTCCGTTGAAACCGCAGAGTTTGATCTCGAAAAAGA
GGATATTGCTACAGATTTAGTTCAAGAACTTGGACTAGAACAACAGGTTCAAGATGACCTGCAAAAAAATCTTGATGCCA
CGCAACTCTATTTAGGTGAAATAGGGTTTTCCCCCCTGCTTAGCGCAGAAGAAGAAGTTTACTTTTCTCGTAAAGCCTTA
AAAGGCTGTGAAAAATCCCGTAATCGCATGATTGAAAGTAATCTTAGACTTGTTGTTAAGATTGCACGTCGTTATAACAA
CCGAGGATTAGCACTCTTAGATTTAATCGAAGAAGGGAACTTAGGACTTATCCGCGCGGTAGAAAAATTTGATCCCGAAA
GAGGTTTTCGTTTCTCAACCTATGCAACTTGGTGGATTCGTCAAACGATTGAACGTGCAATTATGAATCAAACTCGCACC
ATTCGTTTACCGATTCATGTTGTTAAAGAGTTGAACGTATATTTAAGAACCGCTCGGGAATTAGCGCAAAAATTGGATCA
CGAACCGACAGCTGAAGAAATTGCTGAAAAATTGCAAGTCCCTAGTGCAGATGTCAGCCGTATGTTGAAGTTGAATGAAA
AGATTACCTCAGTCGATACGCCTTTGGGGGGCGATAATGACAAAGCGCTGATCGATATATTGGCCGATGATGACAGTGTA
GGCCCTGACTATAAGGTACAGGATGAAGATATCTCAAACTCGGTAGTGAAATGGCTCAATGAGTTAAATACTAAACAAAG
AGAGGTATTAGCTCGTCGTTTTGGATTATTAGGCTATGAGCCATCGACATTGGAAGATGTCGGGGCAGAGATAGGGTTAA
CCCGTGAGCGTGTTCGTCAAATTCAAGTTGAAGCCTTAAAACGTCTACGTGATTTACTCGGTTCACAAGGCCTATCTGTT
GAAGCGTTGTTTAGAAATTAA

Upstream 100 bases:

>100_bases
TTATTTGCCTAAGCAATGATTGCTTAGGGCCTTGTTGGCAAATGGAGGATAGGAAATTGCAATAGTTCAAGCAGTTTAAT
TAGGTTTAGGAGATTTGATC

Downstream 100 bases:

>100_bases
TGTTATTGGGCAAAGTAAGACGGTAATGATCAAACAATTTAAGTCCCTATTAAAAACGCCCACAATAGTGGGCGTTTTGT
TTTTTAATTTAATTGATTAG

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSRINSTAAEELVDFSVETAEFDLEKEDIATDLVQELGLEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKAL
KGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRT
IRLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVPSADVSRMLKLNEKITSVDTPLGGDNDKALIDILADDDSV
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGSQGLSV
EALFRN

Sequences:

>Translated_326_residues
MSRINSTAAEELVDFSVETAEFDLEKEDIATDLVQELGLEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKAL
KGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRT
IRLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVPSADVSRMLKLNEKITSVDTPLGGDNDKALIDILADDDSV
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGSQGLSV
EALFRN
>Mature_325_residues
SRINSTAAEELVDFSVETAEFDLEKEDIATDLVQELGLEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKALK
GCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTI
RLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVPSADVSRMLKLNEKITSVDTPLGGDNDKALIDILADDDSVG
PDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGSQGLSVE
ALFRN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=320, Percent_Identity=74.375, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=43.3628318584071, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=29.7491039426523, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788231, Length=205, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37118; Mature: 36986

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRINSTAAEELVDFSVETAEFDLEKEDIATDLVQELGLEQQVQDDLQKNLDATQLYLGE
CCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
IGFSPLLSAEEEVYFSRKALKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCC
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARE
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
LAQKLDHEPTAEEIAEKLQVPSADVSRMLKLNEKITSVDTPLGGDNDKALIDILADDDSV
HHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCC
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQ
CCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
IQVEALKRLRDLLGSQGLSVEALFRN
HHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
SRINSTAAEELVDFSVETAEFDLEKEDIATDLVQELGLEQQVQDDLQKNLDATQLYLGE
CCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
IGFSPLLSAEEEVYFSRKALKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCC
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARE
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
LAQKLDHEPTAEEIAEKLQVPSADVSRMLKLNEKITSVDTPLGGDNDKALIDILADDDSV
HHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCC
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQ
CCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
IQVEALKRLRDLLGSQGLSVEALFRN
HHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]