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The map label for this gene is recA

Identifier: 120598088

GI number: 120598088

Start: 1438954

End: 1440027

Strand: Direct

Name: recA

Synonym: Sputw3181_1265

Alternate gene names: 120598088

Gene position: 1438954-1440027 (Clockwise)

Preceding gene: 120598086

Following gene: 120598089

Centisome position: 30.56

GC content: 45.16

Gene sequence:

>1074_bases
ATGAAGGTCGATCCAAACAAAGAGAAAGCACTCGCAGCGGTATTGAGCCAGATTGAAAAGCAATTTGGTAAAGGTTCTAT
CATGAAGCTGGGCGAAGATCGCTCTATGGATGTCGAAACCATTTCTACTGGTTCGTTATCTCTAGATGTCGCTTTAGGTG
CTGGTGGCTTGCCAATGGGGCGTATTGTTGAGATCTATGGTCCTGAATCATCTGGTAAAACGACACTGACTTTAGAAGTG
ATTGCCGCTGCGCAGCGTGAAGGTAAAACCTGTGCTTTTATCGATGCAGAACATGCGTTAGACCCAATCTATGCTAAAAA
ATTGGGCGTAGATATTGATAACCTGCTGTGTTCACAACCGGATACTGGCGAGCAAGCGCTTGAGATTTGTGATGCTTTAA
CTCGCTCAGGCGCTGTTGACGTTATCGTAGTCGACTCCGTGGCTGCATTAACGCCTAAAGCTGAAATCGAAGGCGAAATT
GGTGATTCTCACATGGGCCTAGCGGCGCGTATGATGAGCCAAGCCATGCGTAAACTTGCTGGTAACTTAAAGCAATCTAA
CACCTTACTTATCTTTATCAACCAAATTCGGATGAAGATTGGTGTGATGTTCGGTAACCCAGAAACGACAACGGGTGGTA
ACGCACTGAAGTTCTATGCTTCTGTTCGTTTGGATATTCGCCGCACAGGTGCCATTAAAGACGGCGATGAAGTTGTCGGT
AACGAAACTCGCGTTAAAGTGGTGAAAAACAAAGTTGCGGCTCCATTTAAGCAAGCTGAGTTCCAAATCCTTTACGGCCA
AGGTATTAACCGAACAGGTGAGTTAGTCGACTTAGGTGTAGCCCATAAGCTGATCGAGAAAGCCGGTGCTTGGTACAGCT
ATAAAGGTGATAAAATTGGCCAAGGTCGTGCAAATGCGGGTAAATATCTGACTGAAAACCCAGCAATTGCCGCCGAAATA
GACAAGACATTACGTGAGTTACTGTTAAGTAATCCAAGTGCCCTAGCATCATCTGCATCTGATGATGAAAGCACAGAAGG
TAATATTGACTTAGAGACAGGCGAAATATTCTGA

Upstream 100 bases:

>100_bases
GCATATTAAGACAGAAAGCCTCGCTTTTTGAATTGCCCGCTTTACTAAATTGAATATCACTTTTTGACAGCCTAGATGCT
GCCTAGATGAGGGAACAGGA

Downstream 100 bases:

>100_bases
GTCAGTCCGCATACCAAATCGCGGTAGCGCTGCTAGCGCGCCGCGATTATTCCCGCCAACAAATTCGTGATAAATTGCTC
GATAAAGGTTTCGAACTAAA

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKVDPNKEKALAAVLSQIEKQFGKGSIMKLGEDRSMDVETISTGSLSLDVALGAGGLPMGRIVEIYGPESSGKTTLTLEV
IAAAQREGKTCAFIDAEHALDPIYAKKLGVDIDNLLCSQPDTGEQALEICDALTRSGAVDVIVVDSVAALTPKAEIEGEI
GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKDGDEVVG
NETRVKVVKNKVAAPFKQAEFQILYGQGINRTGELVDLGVAHKLIEKAGAWYSYKGDKIGQGRANAGKYLTENPAIAAEI
DKTLRELLLSNPSALASSASDDESTEGNIDLETGEIF

Sequences:

>Translated_357_residues
MKVDPNKEKALAAVLSQIEKQFGKGSIMKLGEDRSMDVETISTGSLSLDVALGAGGLPMGRIVEIYGPESSGKTTLTLEV
IAAAQREGKTCAFIDAEHALDPIYAKKLGVDIDNLLCSQPDTGEQALEICDALTRSGAVDVIVVDSVAALTPKAEIEGEI
GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKDGDEVVG
NETRVKVVKNKVAAPFKQAEFQILYGQGINRTGELVDLGVAHKLIEKAGAWYSYKGDKIGQGRANAGKYLTENPAIAAEI
DKTLRELLLSNPSALASSASDDESTEGNIDLETGEIF
>Mature_357_residues
MKVDPNKEKALAAVLSQIEKQFGKGSIMKLGEDRSMDVETISTGSLSLDVALGAGGLPMGRIVEIYGPESSGKTTLTLEV
IAAAQREGKTCAFIDAEHALDPIYAKKLGVDIDNLLCSQPDTGEQALEICDALTRSGAVDVIVVDSVAALTPKAEIEGEI
GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKDGDEVVG
NETRVKVVKNKVAAPFKQAEFQILYGQGINRTGELVDLGVAHKLIEKAGAWYSYKGDKIGQGRANAGKYLTENPAIAAEI
DKTLRELLLSNPSALASSASDDESTEGNIDLETGEIF

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=342, Percent_Identity=87.1345029239766, Blast_Score=613, Evalue=1e-177,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 38048; Mature: 38048

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDPNKEKALAAVLSQIEKQFGKGSIMKLGEDRSMDVETISTGSLSLDVALGAGGLPMG
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCC
RIVEIYGPESSGKTTLTLEVIAAAQREGKTCAFIDAEHALDPIYAKKLGVDIDNLLCSQP
CEEEEECCCCCCCEEEEEEEEEHHHCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCC
DTGEQALEICDALTRSGAVDVIVVDSVAALTPKAEIEGEIGDSHMGLAARMMSQAMRKLA
CCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHH
GNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKDGDEVVG
CCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHCC
NETRVKVVKNKVAAPFKQAEFQILYGQGINRTGELVDLGVAHKLIEKAGAWYSYKGDKIG
CCCEEEEHHHHHCCCCHHCCEEEEECCCCCCCCCEEHHHHHHHHHHHHCCEEEECCCCCC
QGRANAGKYLTENPAIAAEIDKTLRELLLSNPSALASSASDDESTEGNIDLETGEIF
CCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MKVDPNKEKALAAVLSQIEKQFGKGSIMKLGEDRSMDVETISTGSLSLDVALGAGGLPMG
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCC
RIVEIYGPESSGKTTLTLEVIAAAQREGKTCAFIDAEHALDPIYAKKLGVDIDNLLCSQP
CEEEEECCCCCCCEEEEEEEEEHHHCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCC
DTGEQALEICDALTRSGAVDVIVVDSVAALTPKAEIEGEIGDSHMGLAARMMSQAMRKLA
CCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHH
GNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKDGDEVVG
CCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHCC
NETRVKVVKNKVAAPFKQAEFQILYGQGINRTGELVDLGVAHKLIEKAGAWYSYKGDKIG
CCCEEEEHHHHHCCCCHHCCEEEEECCCCCCCCCEEHHHHHHHHHHHHCCEEEECCCCCC
QGRANAGKYLTENPAIAAEIDKTLRELLLSNPSALASSASDDESTEGNIDLETGEIF
CCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]