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The map label for this gene is ispD

Identifier: 120598080

GI number: 120598080

Start: 1430095

End: 1430856

Strand: Direct

Name: ispD

Synonym: Sputw3181_1257

Alternate gene names: 120598080

Gene position: 1430095-1430856 (Clockwise)

Preceding gene: 120598079

Following gene: 120598081

Centisome position: 30.37

GC content: 47.11

Gene sequence:

>762_bases
ATGCGTATGGATATCGCATTAGAAGAAAGACCCCATTTAGAACATCAGCCGCAAATGATGATAAAAGCAAATCCAGATCC
CCAAAGCGTTGTCGCGATCGTGCCCGCAGCAGGAATTGGTAGCCGCATGGGGGCCGATAAACCCAAGCAATATCTTGAGG
TATTAGGTCAAAGTATTCTTGCCCATACGTTAGATACACTATTGTCGCATCAACAAATCACCCAAGTGATTATTGCACTA
CATCCTAATGACTCATTTTTTTACGCATTACCACAAGCGCAACATCCAAAACTTATCACAGTGCTTGGTGGCGGTGAGCG
TGCTGATTCAGTGTTGGCGGCGTTGATGAAAGCGCCTGAAGATAGTTGGGCCTTGGTACATGACGCAGCAAGGCCCTGTT
TAACCTTGGTTGATATCGATAAATTACTTGTGTCACGTGCCGATTTCCCCCATGGGGCGATATTGGCAATGCCAGTGCGT
GATACGATGAAGCGTGCTAATCAAAAAGGGGAAATAAGTGCAACTGTTTGCCGCGATAATTTATGGCATGCGTTGACGCC
GCAATTATTTCCAACATCTGAGTTGAAAATGCATCTTCAAGCGGCAATCCTAGCTGGTGTAACGGTTACGGATGAAGCCT
CTGCCATGGAATGGGCAGGGATATCGCCGGGGCTGGTTTCTGGCCGTGCCGACAATATCAAAGTCACACATCCCGATGAT
CTGGAGCTGGCTGAACTCTTTTTAATGCGCGTTGCAAAATAG

Upstream 100 bases:

>100_bases
GCGCGCGTAACGAGCTCGGTATGGTAAAAGAAGGTGAAACTTTCTACCGTGTGGTGGGTGGTGAACGCTCAGTATCGAGT
CCTTCTCAGTGACGGAGTGA

Downstream 100 bases:

>100_bases
TTTATCTAGCATGTTACTAAAGTAAGTTAGGAAATAATAAATGAAAATCCGTATCGGTCATGGTTTCGATGTTCACAAAT
TTGGCGAAGTTCGGCCCTTA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRMDIALEERPHLEHQPQMMIKANPDPQSVVAIVPAAGIGSRMGADKPKQYLEVLGQSILAHTLDTLLSHQQITQVIIAL
HPNDSFFYALPQAQHPKLITVLGGGERADSVLAALMKAPEDSWALVHDAARPCLTLVDIDKLLVSRADFPHGAILAMPVR
DTMKRANQKGEISATVCRDNLWHALTPQLFPTSELKMHLQAAILAGVTVTDEASAMEWAGISPGLVSGRADNIKVTHPDD
LELAELFLMRVAK

Sequences:

>Translated_253_residues
MRMDIALEERPHLEHQPQMMIKANPDPQSVVAIVPAAGIGSRMGADKPKQYLEVLGQSILAHTLDTLLSHQQITQVIIAL
HPNDSFFYALPQAQHPKLITVLGGGERADSVLAALMKAPEDSWALVHDAARPCLTLVDIDKLLVSRADFPHGAILAMPVR
DTMKRANQKGEISATVCRDNLWHALTPQLFPTSELKMHLQAAILAGVTVTDEASAMEWAGISPGLVSGRADNIKVTHPDD
LELAELFLMRVAK
>Mature_253_residues
MRMDIALEERPHLEHQPQMMIKANPDPQSVVAIVPAAGIGSRMGADKPKQYLEVLGQSILAHTLDTLLSHQQITQVIIAL
HPNDSFFYALPQAQHPKLITVLGGGERADSVLAALMKAPEDSWALVHDAARPCLTLVDIDKLLVSRADFPHGAILAMPVR
DTMKRANQKGEISATVCRDNLWHALTPQLFPTSELKMHLQAAILAGVTVTDEASAMEWAGISPGLVSGRADNIKVTHPDD
LELAELFLMRVAK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=54.7085201793722, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 27570; Mature: 27570

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMDIALEERPHLEHQPQMMIKANPDPQSVVAIVPAAGIGSRMGADKPKQYLEVLGQSIL
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AHTLDTLLSHQQITQVIIALHPNDSFFYALPQAQHPKLITVLGGGERADSVLAALMKAPE
HHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
DSWALVHDAARPCLTLVDIDKLLVSRADFPHGAILAMPVRDTMKRANQKGEISATVCRDN
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCEEEEEECCC
LWHALTPQLFPTSELKMHLQAAILAGVTVTDEASAMEWAGISPGLVSGRADNIKVTHPDD
CHHHCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHCCCCCCCCCCCCCCEEEECCCC
LELAELFLMRVAK
HHHHHHHHHHHCC
>Mature Secondary Structure
MRMDIALEERPHLEHQPQMMIKANPDPQSVVAIVPAAGIGSRMGADKPKQYLEVLGQSIL
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AHTLDTLLSHQQITQVIIALHPNDSFFYALPQAQHPKLITVLGGGERADSVLAALMKAPE
HHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
DSWALVHDAARPCLTLVDIDKLLVSRADFPHGAILAMPVRDTMKRANQKGEISATVCRDN
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCEEEEEECCC
LWHALTPQLFPTSELKMHLQAAILAGVTVTDEASAMEWAGISPGLVSGRADNIKVTHPDD
CHHHCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHCCCCCCCCCCCCCCEEEECCCC
LELAELFLMRVAK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]