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The map label for this gene is mazG

Identifier: 120598076

GI number: 120598076

Start: 1425506

End: 1426432

Strand: Direct

Name: mazG

Synonym: Sputw3181_1253

Alternate gene names: 120598076

Gene position: 1425506-1426432 (Clockwise)

Preceding gene: 120598073

Following gene: 120598077

Centisome position: 30.28

GC content: 47.14

Gene sequence:

>927_bases
ATGACTTCCCACACGGCGCCATTAACCTCATTTTCTACTACTGAACCTTCTCTCACTGAAACTTCTACAACGGAACCATC
TACAACGGAACTGTCTATAACAGATGTAGAGCCGCTACTTAAAATAATGGAAAAACTGCGTGATCCGCACACGGGCTGTC
CTTGGGATAAAGCGCAAACCTTTCAAACCATAGTGCCTTTTACCTTAGAGGAGGCCTATGAAGTTGCCGATACCATAGAA
CGTTTAGCCTGGGATGAGTTACCCGATGAACTCGGGGATTTGTTATTTCAAATCGTATTCTATTGTCAGCTCGGCAAAGA
GCAGGGGTTATTTGATTTTTCGAGCGTGATTGAGAAAATCAGCGACAAGTTAACCCGTCGTCATCCCCATGTGTTTGGTG
AGCAAAATGTACAAATTGGCGATAGCGCCAAAGCGATGAAAGCCAATTGGGAGGCGATTAAAGCGATTGAGCGTGAACAA
AAGGCACAGCAAGTGGCGGGTGAAACCCAAGCCGATATTATTGTTTCAGCGCTAGACAATATTCCTCGCTCACAACCCGC
GCTGTCACGTTCAATCAAAATTCAGCAAAGGGTGGCGCGAGTCGGGTTTGATTGGGCCGAGCTTGAGCCCGTGGTCGCTA
AAATCCATGAAGAAATCGATGAAGTCTTGCATGAGGTGAAGCAACCAGAGCACGACCAAGCTAAAGTGCAGTCCGAAATG
GGCGATTTGCTGTTTGCCGTAGTGAATTTAGCTCGACATCTTAAGGTCGATCCTGAACAAGCGTTGCGCCAAGCCAATCT
TAAATTTGAACGCCGTTTTCGCGGCGTTGAACAGTTGGCCAGTGACAGCGGTAAAAGCATGGAAGAGCACACATTAATGG
AGCTTGATGCCTATTGGGACCGAGTAAAAGCGACGGAGACGAGATAA

Upstream 100 bases:

>100_bases
TTTATTATAGCCTTTCGCTAATAAGCACTGCTGAGGCGTATTCTTTTTGTTCTTCCTGAGTATTAGCCTGTTTTAGCATT
TATCTTTAAAGGACATCAGC

Downstream 100 bases:

>100_bases
ACGGCAAATTATCCCGATTGGTGTTTGTCGAATCGCGCCGCCTTTGGTATACTACTGCCCCGTCCTAGTGCTTTCTTACA
AGCACGTATTTTCAAACTCA

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTSHTAPLTSFSTTEPSLTETSTTEPSTTELSITDVEPLLKIMEKLRDPHTGCPWDKAQTFQTIVPFTLEEAYEVADTIE
RLAWDELPDELGDLLFQIVFYCQLGKEQGLFDFSSVIEKISDKLTRRHPHVFGEQNVQIGDSAKAMKANWEAIKAIEREQ
KAQQVAGETQADIIVSALDNIPRSQPALSRSIKIQQRVARVGFDWAELEPVVAKIHEEIDEVLHEVKQPEHDQAKVQSEM
GDLLFAVVNLARHLKVDPEQALRQANLKFERRFRGVEQLASDSGKSMEEHTLMELDAYWDRVKATETR

Sequences:

>Translated_308_residues
MTSHTAPLTSFSTTEPSLTETSTTEPSTTELSITDVEPLLKIMEKLRDPHTGCPWDKAQTFQTIVPFTLEEAYEVADTIE
RLAWDELPDELGDLLFQIVFYCQLGKEQGLFDFSSVIEKISDKLTRRHPHVFGEQNVQIGDSAKAMKANWEAIKAIEREQ
KAQQVAGETQADIIVSALDNIPRSQPALSRSIKIQQRVARVGFDWAELEPVVAKIHEEIDEVLHEVKQPEHDQAKVQSEM
GDLLFAVVNLARHLKVDPEQALRQANLKFERRFRGVEQLASDSGKSMEEHTLMELDAYWDRVKATETR
>Mature_307_residues
TSHTAPLTSFSTTEPSLTETSTTEPSTTELSITDVEPLLKIMEKLRDPHTGCPWDKAQTFQTIVPFTLEEAYEVADTIER
LAWDELPDELGDLLFQIVFYCQLGKEQGLFDFSSVIEKISDKLTRRHPHVFGEQNVQIGDSAKAMKANWEAIKAIEREQK
AQQVAGETQADIIVSALDNIPRSQPALSRSIKIQQRVARVGFDWAELEPVVAKIHEEIDEVLHEVKQPEHDQAKVQSEMG
DLLFAVVNLARHLKVDPEQALRQANLKFERRFRGVEQLASDSGKSMEEHTLMELDAYWDRVKATETR

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=274, Percent_Identity=53.6496350364963, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 34952; Mature: 34821

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHTAPLTSFSTTEPSLTETSTTEPSTTELSITDVEPLLKIMEKLRDPHTGCPWDKAQT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHH
FQTIVPFTLEEAYEVADTIERLAWDELPDELGDLLFQIVFYCQLGKEQGLFDFSSVIEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SDKLTRRHPHVFGEQNVQIGDSAKAMKANWEAIKAIEREQKAQQVAGETQADIIVSALDN
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
IPRSQPALSRSIKIQQRVARVGFDWAELEPVVAKIHEEIDEVLHEVKQPEHDQAKVQSEM
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GDLLFAVVNLARHLKVDPEQALRQANLKFERRFRGVEQLASDSGKSMEEHTLMELDAYWD
HHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RVKATETR
HHHHCCCC
>Mature Secondary Structure 
TSHTAPLTSFSTTEPSLTETSTTEPSTTELSITDVEPLLKIMEKLRDPHTGCPWDKAQT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHH
FQTIVPFTLEEAYEVADTIERLAWDELPDELGDLLFQIVFYCQLGKEQGLFDFSSVIEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SDKLTRRHPHVFGEQNVQIGDSAKAMKANWEAIKAIEREQKAQQVAGETQADIIVSALDN
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
IPRSQPALSRSIKIQQRVARVGFDWAELEPVVAKIHEEIDEVLHEVKQPEHDQAKVQSEM
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GDLLFAVVNLARHLKVDPEQALRQANLKFERRFRGVEQLASDSGKSMEEHTLMELDAYWD
HHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RVKATETR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]