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The map label for this gene is pyrG

Identifier: 120598077

GI number: 120598077

Start: 1426549

End: 1428186

Strand: Direct

Name: pyrG

Synonym: Sputw3181_1254

Alternate gene names: 120598077

Gene position: 1426549-1428186 (Clockwise)

Preceding gene: 120598076

Following gene: 120598078

Centisome position: 30.3

GC content: 45.3

Gene sequence:

>1638_bases
ATGACTACAAGGTATATCTTCGTTACTGGTGGCGTTGTTTCATCACTAGGTAAAGGCATTGCAGCAGCATCATTAGCTGC
AATTTTAGAGGCCCGCGGTCTCAATGTAACCATCATGAAACTGGACCCTTACATTAACGTTGATCCGGGTACCATGAGCC
CAACCCAACATGGCGAAGTATTTGTTACTGAAGACGGTGCAGAAACAGATCTCGACTTGGGTCACTACGAGCGTTTTATC
CGTACTAAGATGAATCGTCGTAACAACTTCACTACTGGTCGTATTTACGAAGAAGTACTACGTAAAGAACGTCGTGGCGA
TTATTTGGGCGCAACCATTCAGGTTATTCCACATATCACTAATGCGATTAAAGAAAAAGTATTAGCAGGTGGCGAAGGCC
ATGATGTGGCTATCGTCGAAATCGGCGGTACTGTGGGTGATATCGAGTCCTTGCCGTTCCTTGAGTCTATCCGTCAGCTC
GGCGTTGAGCTTGGCCGCGATCGTACTCTGTTCATGCATTTAACCTTAGTGCCTTTCTTAGGTGCGGCTGGCGAAGTGAA
AACTAAGCCAACTCAACACTCAGTAAAAGAGTTGCGTTCTATTGGTATTGCACCCGATGTGTTGATTTGCCGTGGCGATC
GCGCAATTCCAGCAAACGAGCGCGCGAAGATTTCTCTATTCTGTAATGTTGAAGAGCGTGCGGTTATTTCATTAAAAGAT
GTTGATAGTATTTATAAAATCCCAGCACTGCTGCGTTCACAAGGTTTGGATGACTTAGTGGTTAAGCGTTTCGGTTTAGA
ATGCCGTGAAGCTGACTTATCTGAGTGGGAAAATGTGATTTACCAAGAAGCTAATCCAAACGGCGAAGTCGTGATTGGTA
TGGTGGGTAAATACATTGAACTGCCAGATGCCTATAAGTCAGTCAACGAAGCATTAAAACACGCAGGCCTTAAAAACCGC
GTATCTGTGACCATCAAGTATATTGATTCACAAACCGTAGAAGCTAAAGGCGATGAAGTCCTACAGGGCTTAGACGGTAT
CTTAGTACCAGGTGGCTTCGGCGAGCGTGGTGTTGAAGGTAAGATACTGGCGGCAAAATTTGCCCGTGAAAACAAACTAC
CTTATTTCGGTATTTGTTTAGGTATGCAAGTTGCTCTGATTGAGTTTGCCCGTAATGTTGCTGGTATGACTGATGCTCAT
TCAACTGAATTTAATAAAGAAACGCCATTCCCAGTAGTAGGTTTAATCACTGAATGGGTTGATGAGGAAGGTAATGTTGA
GCAACGCCATGAGGCGTCTGACTTAGGTGGCACTATGCGTTTAGGCGCACAATTGTGCCATTTACTTGAAGGCTCTAAAG
CGGCACAAGCCTACAAAGGCAATAGCTGTATTGAACGCCATCGTCACCGTTACGAAGTGAACAACAAGTACAGAGAACGC
TTAGAGCAAGCTGGAATGGTGTTCAGTGGTTTATCTTCCGATCGCAAGCTGGTAGAAATGATCGAATTAAAAGATCACCC
ATGGTTTGTTGCGGGTCAATTCCACCCTGAGTTCACCTCAACGCCACGCGATGGTCACCCATTGTTCGAAGGCTTTATTG
CCGCAGCGAGCGCACACCAAAAACGTGATCTGAAGTAA

Upstream 100 bases:

>100_bases
ATTGGTGTTTGTCGAATCGCGCCGCCTTTGGTATACTACTGCCCCGTCCTAGTGCTTTCTTACAAGCACGTATTTTCAAA
CTCATTTTCTCAGGTTCAGC

Downstream 100 bases:

>100_bases
CAATATTTAAGGCCAGAACCGCGACAGTGGGACTGGCCTTTTGTTACACTCAAGCCGCTTTGATATGAAAAGAACTCGTA
CCCATGTGTATAAATGATAC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIELPDAYKSVNEALKHAGLKNR
VSVTIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEGKILAAKFARENKLPYFGICLGMQVALIEFARNVAGMTDAH
STEFNKETPFPVVGLITEWVDEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNSCIERHRHRYEVNNKYRER
LEQAGMVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFIAAASAHQKRDLK

Sequences:

>Translated_545_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIELPDAYKSVNEALKHAGLKNR
VSVTIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEGKILAAKFARENKLPYFGICLGMQVALIEFARNVAGMTDAH
STEFNKETPFPVVGLITEWVDEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNSCIERHRHRYEVNNKYRER
LEQAGMVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFIAAASAHQKRDLK
>Mature_544_residues
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLG
VELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKDV
DSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIELPDAYKSVNEALKHAGLKNRV
SVTIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEGKILAAKFARENKLPYFGICLGMQVALIEFARNVAGMTDAHS
TEFNKETPFPVVGLITEWVDEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNSCIERHRHRYEVNNKYRERL
EQAGMVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFIAAASAHQKRDLK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=44.3447037701975, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=560, Percent_Identity=44.2857142857143, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI28559083, Length=560, Percent_Identity=44.2857142857143, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI221316689, Length=560, Percent_Identity=44.2857142857143, Blast_Score=464, Evalue=1e-130,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=77.4311926605505, Blast_Score=854, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=611, Percent_Identity=38.4615384615385, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=43.2337434094903, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=41.2280701754386, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=45.2423698384201, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=43.9363817097416, Blast_Score=406, Evalue=1e-113,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60182; Mature: 60050

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRTLFMHLTLVPFL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEHHH
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIE
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCHHHHHHHHHEECCCCCCCEEEEECCCHHC
LPDAYKSVNEALKHAGLKNRVSVTIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCC
KILAAKFARENKLPYFGICLGMQVALIEFARNVAGMTDAHSTEFNKETPFPVVGLITEWV
CEEEEHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
DEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNSCIERHRHRYEVNNKYRER
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHH
LEQAGMVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFIAAASAHQ
HHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KRDLK
HCCCC
>Mature Secondary Structure 
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRTLFMHLTLVPFL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEHHH
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIE
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCHHHHHHHHHEECCCCCCCEEEEECCCHHC
LPDAYKSVNEALKHAGLKNRVSVTIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCC
KILAAKFARENKLPYFGICLGMQVALIEFARNVAGMTDAHSTEFNKETPFPVVGLITEWV
CEEEEHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
DEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNSCIERHRHRYEVNNKYRER
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHH
LEQAGMVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFIAAASAHQ
HHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KRDLK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA