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The map label for this gene is radA [H]

Identifier: 120598020

GI number: 120598020

Start: 1350025

End: 1351389

Strand: Direct

Name: radA [H]

Synonym: Sputw3181_1197

Alternate gene names: 120598020

Gene position: 1350025-1351389 (Clockwise)

Preceding gene: 120598018

Following gene: 120598022

Centisome position: 28.67

GC content: 48.06

Gene sequence:

>1365_bases
ATGGCAAAGAATAAAACTGCGTACGTGTGTAATGAGTGTGGGCAAGACTTTCCTCGTTGGCAAGGCCAATGTAGCGCCTG
CCAAGAATGGAATACGATTACCGAAGTTAGACTCGGTGCTGCTTCGCCTGGGCGAGGGACTAAGTTTGCAGGTTATGCAG
GGGCAAGTAGTAGTGAAGTCAAAACCTTAGATCAAATCGATTTGAATGCGTTACCGCGAATTTTGAGTCACTTTGGAGAA
CTCGACCGAGTGCTGGGGGGCGGGATTGTCCCGGGTTCAGCGATTCTTATCGGTGGTCACCCTGGAGCCGGTAAAAGTAC
CCTGTTGTTGCAAACGCTCTGTTATTTGGCTGAACAAATGCCTGCATTATACGTAACGGGTGAAGAGTCTTTACAGCAGG
TTGCCATGCGCGCCCATCGTTTGGGTTTACCGACCAATAAATTACGGATGTTATCGGAAACCAGTGTCGAGCAAATTTGT
GAGGTTGCCCTAAAAGAATTACCTAAGGTGATTGTGGTTGATTCGATTCAGGTCATGCATATGAGTGATGTGGCATCAAG
TCCTGGCAGTGTATCGCAGGTACGAGAGTCTGCTTCTTATTTGACGCGTTTTGCGAAACAAAATGGTATAGCCGTGATTA
TGGTGGGCCATGTCACTAAGGACGGTAGCCTTGCAGGCCCTAAGGTGCTTGAGCATTGTATTGATTGTTCAGTGATGTTT
GAGGGGGATAGTGACAGTCGTTACCGTACCTTGCGTTCCCACAAAAACCGTTTCGGTGCAATCAATGAGTTAGGTGTATT
TGCCATGACAGAGCGCGGCTTAAAAGAAGTCGCTAATCCTTCAGCTATCTTCCTTTCCCGTGGTGAAGAGGAATCGTCTG
GTTCTTTAGTGATGGTGGTGTGGGAAGGTACGCGTCCGCTGTTAGTAGAACTGCAAGTGCTGGTGGATAACTCGGCTATG
TCTAATCCGCGCCGCGTGGCTGTTGGGATGGATGCAAACCGTTTAGCCATGTTACTGGCGGTTATGCATCGCCATGGCGG
TTTGCAAATGTCGGATCAGGATGTGTTTGTTAACGTAGTCGGTGGCGTTAAAGTGACGGAAACCAGTGCGGACTTAACCC
TATTACTGGCAATGGTATCGAGTTTTCGTGGCGAGATTTTACCCAGTGATTTGGTCGCATTTGGTGAAGTCGGGTTATCG
GGCGAAATTCGGCCAGTGCCAAATGGACAAGAGCGATTAATCGAAGCGGCTAAACATGGTTTTAAACGAGCGATAGTCCC
TAAGGCTAATGTGCCTAAAAAGCCTCCTATAGGGATGGAAGTTATCGGTGTTTCAAAACTATCAGAAGCCTTAGAGGCGA
TTTAG

Upstream 100 bases:

>100_bases
AGCATCTTCAGGTTGTTTGGGTATATGCCGTGCTGTGTTAGTCTTATGTCGGCAGTATTCACAGTTACTTAAATTTATTT
TATACAGATTTTAATGAGTT

Downstream 100 bases:

>100_bases
CTTAGTGGTTGAGTGCACCCCAATATAAGCAATGCTTATATTGGGGTTAATAAGTGCGCTTAATGGGCGTTATTGTGTTC
TTCAATAAAAAATGCAAGTT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRGTKFAGYAGASSSEVKTLDQIDLNALPRILSHFGE
LDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCYLAEQMPALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQIC
EVALKELPKVIVVDSIQVMHMSDVASSPGSVSQVRESASYLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVVWEGTRPLLVELQVLVDNSAM
SNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGEILPSDLVAFGEVGLS
GEIRPVPNGQERLIEAAKHGFKRAIVPKANVPKKPPIGMEVIGVSKLSEALEAI

Sequences:

>Translated_454_residues
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRGTKFAGYAGASSSEVKTLDQIDLNALPRILSHFGE
LDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCYLAEQMPALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQIC
EVALKELPKVIVVDSIQVMHMSDVASSPGSVSQVRESASYLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVVWEGTRPLLVELQVLVDNSAM
SNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGEILPSDLVAFGEVGLS
GEIRPVPNGQERLIEAAKHGFKRAIVPKANVPKKPPIGMEVIGVSKLSEALEAI
>Mature_453_residues
AKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRGTKFAGYAGASSSEVKTLDQIDLNALPRILSHFGEL
DRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCYLAEQMPALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQICE
VALKELPKVIVVDSIQVMHMSDVASSPGSVSQVRESASYLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMFE
GDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVVWEGTRPLLVELQVLVDNSAMS
NPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGEILPSDLVAFGEVGLSG
EIRPVPNGQERLIEAAKHGFKRAIVPKANVPKKPPIGMEVIGVSKLSEALEAI

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=455, Percent_Identity=76.4835164835165, Blast_Score=718, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48719; Mature: 48588

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRGTKFAGYAGASSSEV
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCHHHEEECCCCCCCCCCCCCCCCCCCCHH
KTLDQIDLNALPRILSHFGELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCYLAEQM
HHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
PALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQICEVALKELPKVIVVDSIQVMH
CEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
MSDVASSPGSVSQVRESASYLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCEEEE
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVV
ECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
WEGTRPLLVELQVLVDNSAMSNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVV
ECCCCCEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
GGVKVTETSADLTLLLAMVSSFRGEILPSDLVAFGEVGLSGEIRPVPNGQERLIEAAKHG
CCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
FKRAIVPKANVPKKPPIGMEVIGVSKLSEALEAI
HHEECCCCCCCCCCCCCCEEEECHHHHHHHHHCC
>Mature Secondary Structure 
AKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRGTKFAGYAGASSSEV
CCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCHHHEEECCCCCCCCCCCCCCCCCCCCHH
KTLDQIDLNALPRILSHFGELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCYLAEQM
HHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
PALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQICEVALKELPKVIVVDSIQVMH
CEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
MSDVASSPGSVSQVRESASYLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCEEEE
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVV
ECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
WEGTRPLLVELQVLVDNSAMSNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVV
ECCCCCEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
GGVKVTETSADLTLLLAMVSSFRGEILPSDLVAFGEVGLSGEIRPVPNGQERLIEAAKHG
CCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
FKRAIVPKANVPKKPPIGMEVIGVSKLSEALEAI
HHEECCCCCCCCCCCCCCEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]