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The map label for this gene is prfC

Identifier: 120598007

GI number: 120598007

Start: 1334096

End: 1335682

Strand: Direct

Name: prfC

Synonym: Sputw3181_1184

Alternate gene names: 120598007

Gene position: 1334096-1335682 (Clockwise)

Preceding gene: 120598006

Following gene: 120598008

Centisome position: 28.33

GC content: 44.99

Gene sequence:

>1587_bases
ATGGCAAACCAAGATTTTCTTAATGAAATCAATAAGCGAAGGACCTTTGCTATCATCTCGCACCCCGACGCGGGTAAAAC
AACCATTACCGAGAAAGTGCTGTTATTCGGAAACGCTTTACAAAAAGCGGGTACGGTTAAAGGTAAAAAATCGGGTCAGC
ATGCAAAGTCTGACTGGATGGAAATGGAAAAAGACCGCGGTATTTCGATTACTACGTCTGTCATGCAATTTCCCTATGGT
GGCGCCCTCGTTAACCTACTCGATACGCCTGGTCACGAAGACTTCTCCGAAGATACTTATCGTACTTTGACTGCGGTTGA
CTCATGTCTGATGGTGATCGACTCTGCAAAGGGTGTCGAAGACCGAACAATCAAGTTGATGGAAGTGACTCGTCTGCGTG
ATACGCCGATCGTGACTTTTATGAACAAACTCGACCGTGATATCCGTGATCCTATCGATTTGATGGATGAAGTCGAAAAC
GTACTGAATATTGCTTGTGCTCCTATTACTTGGCCTATTGGCAGCGGTAAAGAATTTAAAGGCGTATACCATATTCTGCG
TGATGAAGTCGTGTTGTATCAAAGCGGTATGGGTCATACGATTCAAGAACGTCGTGTGATTGAAGGCATTAATAATCCAG
ATCTTGATAAAGCGATTGGTAGTTATGCTGCCGATTTACGTGATGAGATGGAATTAGTGCGCGGTGCGTCGAATGAGTTT
GATCATGCGGCTTTCTTGAAAGGCGAATTGACCCCCGTATTTTTTGGTACTGCTTTAGGTAACTTTGGTGTTGATCATAT
TCTTGATGGCATTGTGGAATGGGCTCCTAAGCCATTACCACGAGAAAGTGACACTCGGGTGATCATGCCTGATGAGGAAA
AGTTCACTGGATTCGTGTTCAAGATCCAAGCAAACATGGATCCCAAACACCGTGACCGTGTTGCCTTTATGCGTGTCTGT
TCAGGCCGTTATGAACAGGGTATGAAGATGCACCATGTGCGTATTGGTAAAGATGTTAACGTCAGTGATGCCTTGACCTT
TATGGCGGGCGATCGTGAGCGTGCCGAAGAAGCCTATCCTGGGGATATCATTGGCTTACATAACCATGGCACCATTCGTA
TTGGTGATACCTTTACTCAAGGTGAGAAATTCCGTTTTACAGGCGTACCTAATTTCGCCCCTGAAATGTTCCGCCGTATT
CGTCTGCGTGATCCACTTAAGCAAAAACAACTACTAAAAGGCTTAGTTCAGTTATCTGAAGAAGGTGCTGTACAGGTATT
CCGTCCTCTTGATACCAATGATTTAATTGTTGGTGCGGTGGGGGTACTACAGTTTGAAGTAGTTGTGGGACGTTTGAAGA
GTGAGTATAACGTCGAGGCGATTTATGAAGGCATTAGCGTGTCGACTGCCCGCTGGGTTTATTGTAAAGATGAGCGTAAG
CTAGAAGAGTTTCGCCGTAAGTGCAGCCAAAACTTGGCGCTTGATGGCGGCGACAACTTGACCTATATTGCGCCAACGAT
GGTTAACCTCAATCTTTCGATGGAAAGATATCCTGATATAGAGTTTGCGAAAACTCGCGAACATTAA

Upstream 100 bases:

>100_bases
CAATTACGATTAACACGACGGATTTGACAAGCGAAGAACTGAAAAGAGAGTACTTCCAAAAGTGTGTACAAATCCGTGTA
CAAACTAAAAGAATTTATAC

Downstream 100 bases:

>100_bases
CTCTGTTAGGGTGATCATAGTTGTTGAAATGGCGGTAATTTACCGCCATTTTTGTATCTAATTTACTTTTCTGGATGCGG
TTATGGCTAATTGGATCACA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MANQDFLNEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYG
GALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVEN
VLNIACAPITWPIGSGKEFKGVYHILRDEVVLYQSGMGHTIQERRVIEGINNPDLDKAIGSYAADLRDEMELVRGASNEF
DHAAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVC
SGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRI
RLRDPLKQKQLLKGLVQLSEEGAVQVFRPLDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERK
LEEFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Sequences:

>Translated_528_residues
MANQDFLNEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYG
GALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVEN
VLNIACAPITWPIGSGKEFKGVYHILRDEVVLYQSGMGHTIQERRVIEGINNPDLDKAIGSYAADLRDEMELVRGASNEF
DHAAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVC
SGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRI
RLRDPLKQKQLLKGLVQLSEEGAVQVFRPLDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERK
LEEFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
>Mature_527_residues
ANQDFLNEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGG
ALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVENV
LNIACAPITWPIGSGKEFKGVYHILRDEVVLYQSGMGHTIQERRVIEGINNPDLDKAIGSYAADLRDEMELVRGASNEFD
HAAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVCS
GRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIR
LRDPLKQKQLLKGLVQLSEEGAVQVFRPLDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKL
EEFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=480, Percent_Identity=27.2916666666667, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.2745098039216, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.9187817258883, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=25.7261410788382, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=28.4883720930233, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI94966754, Length=157, Percent_Identity=29.2993630573248, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=77.6937618147448, Blast_Score=876, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=500, Percent_Identity=25, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.1282051282051, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI48994988, Length=198, Percent_Identity=29.2929292929293, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=488, Percent_Identity=27.0491803278689, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=183, Percent_Identity=30.6010928961749, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.1111111111111, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=163, Percent_Identity=29.4478527607362, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=485, Percent_Identity=26.8041237113402, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=522, Percent_Identity=24.5210727969349, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=28.125, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=28.125, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=153, Percent_Identity=24.1830065359477, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582462, Length=484, Percent_Identity=26.4462809917355, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI221458488, Length=511, Percent_Identity=23.4833659491194, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI78706572, Length=248, Percent_Identity=28.6290322580645, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=31.1688311688312, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=31.1688311688312, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=31.1688311688312, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_SHESW (A1RH82)

Other databases:

- EMBL:   CP000503
- RefSeq:   YP_962581.1
- ProteinModelPortal:   A1RH82
- SMR:   A1RH82
- STRING:   A1RH82
- GeneID:   4661528
- GenomeReviews:   CP000503_GR
- KEGG:   shw:Sputw3181_1184
- NMPDR:   fig|351745.7.peg.1101
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59602; Mature: 59471

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQDFLNEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWM
CCCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKDRGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVE
HHHHCCCCEEEHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVENVLNIACAPITWPIGSGKEFK
CHHEEEEEHHHCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHEEECCEEECCCCCCHHH
GVYHILRDEVVLYQSGMGHTIQERRVIEGINNPDLDKAIGSYAADLRDEMELVRGASNEF
HHHHHHHHCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DHAAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVF
CCHHEECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEE
KIQANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYP
EEEECCCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHHEEECCHHHHHHCCC
GDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSE
CCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
EGAVQVFRPLDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERK
CCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHH
LEEFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHHHHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ANQDFLNEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWM
CCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKDRGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVE
HHHHCCCCEEEHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVENVLNIACAPITWPIGSGKEFK
CHHEEEEEHHHCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHEEECCEEECCCCCCHHH
GVYHILRDEVVLYQSGMGHTIQERRVIEGINNPDLDKAIGSYAADLRDEMELVRGASNEF
HHHHHHHHCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DHAAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVF
CCHHEECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEE
KIQANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYP
EEEECCCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHHEEECCHHHHHHCCC
GDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSE
CCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
EGAVQVFRPLDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERK
CCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHH
LEEFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHHHHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA