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The map label for this gene is truB

Identifier: 120597902

GI number: 120597902

Start: 1218526

End: 1219482

Strand: Direct

Name: truB

Synonym: Sputw3181_1072

Alternate gene names: 120597902

Gene position: 1218526-1219482 (Clockwise)

Preceding gene: 120597901

Following gene: 120597903

Centisome position: 25.88

GC content: 47.02

Gene sequence:

>957_bases
ATGGCTCGTCGTCCGAAAGGCCGTTTTATCGATGGGATAGTGTTACTGGATAAATCCACTGGCATGAGTTCTAACTTTGC
CTTGCAAAGGGTAAAGCGCTTTTTTAATGCGAATAAAGCCGGGCATACGGGTGCACTCGATCCGTTAGCGACAGGTATGT
TACCAGTGTGCCTAGGTGAAGCGACTAAGTTTTCCCAGCACTTATTAGATGCTGATAAACGTTACTTAGTGACAGCAAAA
CTGGGCGAGCGTACCGATACCAGTGATTCAGATGGTGAAGTGGTTCAGACGCGCCCAGTTACATTTACTGAGGCGCAACT
ACTGAGTGCGCTTGAGCACTTTCGTGGTGACACTCAGCAAGTGCCTTCTATGTATTCCGCGCTGAAATATCAGGGGCAGC
CTCTGTATAAATATGCGCGTGAAGGCATAGAAGTGCCGCGAGAATCTCGCCCTATTACGGTATTTGAGCTTAATTTCATC
AGTCTTGAAGGTGATGAGTTGACCTTAGACATTCATTGCTCTAAAGGGACTTATATTCGCACCATCATTGATGATCTTGG
TGAAATGTTAGGTTGTGGCGCCCATGTGATTATGTTGCGACGAACTCAAGTTGCACAGTATCCCTATGCCAAAATGGTTA
CACTAGAACAGCTCGAAGCACTTGTCACCCAAGCACATGAACAACAGATAGATCCGAGTGTATTACTCGATCCTCTCTTA
TTACCCATGGATACGGCAGTGGCTGACTTTCCTGAGGTCAATGTGCCTGAAGCGAGTGCGGCTTATTTGATGCAAGGCCA
AGCGGTACGTGTGACAGGTCTAAAAGCTGACACATTAGTGCGTATCACCTTAGGGGATGCGCATCGATTCGTTGGTATTG
GTGCAATGAATGATGATGGCCTTTTGGCGCCGAAACGGCTCATAGTGATCCATGACGAGCCGATAGTCACAGATTAA

Upstream 100 bases:

>100_bases
AAGACAAGGCAAAACAACAGCAGTTTGGTAGCGCAGATGAAGTCCTTAATGAGGATGAGGGCGCTACCGACGATACAGAT
GATACAAAAGGTAAAGATTA

Downstream 100 bases:

>100_bases
TTCATTCTGCCAATTCGAAATACGCCCGCCTTTTTATTGCGCTAGGGTTGGATTATGGGTAGAATAGCGCGCCCTCTGGC
TGAGTTAGTGATCGGCTAGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEATKFSQHLLDADKRYLVTAK
LGERTDTSDSDGEVVQTRPVTFTEAQLLSALEHFRGDTQQVPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFI
SLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAQYPYAKMVTLEQLEALVTQAHEQQIDPSVLLDPLL
LPMDTAVADFPEVNVPEASAAYLMQGQAVRVTGLKADTLVRITLGDAHRFVGIGAMNDDGLLAPKRLIVIHDEPIVTD

Sequences:

>Translated_318_residues
MARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEATKFSQHLLDADKRYLVTAK
LGERTDTSDSDGEVVQTRPVTFTEAQLLSALEHFRGDTQQVPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFI
SLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAQYPYAKMVTLEQLEALVTQAHEQQIDPSVLLDPLL
LPMDTAVADFPEVNVPEASAAYLMQGQAVRVTGLKADTLVRITLGDAHRFVGIGAMNDDGLLAPKRLIVIHDEPIVTD
>Mature_317_residues
ARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEATKFSQHLLDADKRYLVTAKL
GERTDTSDSDGEVVQTRPVTFTEAQLLSALEHFRGDTQQVPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFIS
LEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAQYPYAKMVTLEQLEALVTQAHEQQIDPSVLLDPLLL
PMDTAVADFPEVNVPEASAAYLMQGQAVRVTGLKADTLVRITLGDAHRFVGIGAMNDDGLLAPKRLIVIHDEPIVTD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=199, Percent_Identity=37.1859296482412, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=64.8387096774194, Blast_Score=426, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17553978, Length=205, Percent_Identity=31.219512195122, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=278, Percent_Identity=26.2589928057554, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=149, Percent_Identity=36.9127516778523, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364189, Length=265, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364187, Length=265, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364185, Length=265, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364183, Length=265, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62471759, Length=265, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17975520, Length=265, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35055; Mature: 34924

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGE
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQHLLDADKRYLVTAKLGERTDTSDSDGEVVQTRPVTFTEAQLLSALEHFRGDTQQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEHHHHHHHHHHHHCCCHHH
VPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFISLEGDELTLDIHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAQYPYAKMVTLEQLEALVTQAHEQQIDPSVLLDPLL
HHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHH
LPMDTAVADFPEVNVPEASAAYLMQGQAVRVTGLKADTLVRITLGDAHRFVGIGAMNDDG
CCCHHHHHCCCCCCCCCCHHHHHHCCCEEEEECCCCCEEEEEEECCCEEEEEEECCCCCC
LLAPKRLIVIHDEPIVTD
CCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
ARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGE
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQHLLDADKRYLVTAKLGERTDTSDSDGEVVQTRPVTFTEAQLLSALEHFRGDTQQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEHHHHHHHHHHHHCCCHHH
VPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFISLEGDELTLDIHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAQYPYAKMVTLEQLEALVTQAHEQQIDPSVLLDPLL
HHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHH
LPMDTAVADFPEVNVPEASAAYLMQGQAVRVTGLKADTLVRITLGDAHRFVGIGAMNDDG
CCCHHHHHCCCCCCCCCCHHHHHHCCCEEEEECCCCCEEEEEEECCCEEEEEEECCCCCC
LLAPKRLIVIHDEPIVTD
CCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA