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The map label for this gene is rbfA

Identifier: 120597901

GI number: 120597901

Start: 1218077

End: 1218526

Strand: Direct

Name: rbfA

Synonym: Sputw3181_1071

Alternate gene names: 120597901

Gene position: 1218077-1218526 (Clockwise)

Preceding gene: 120597900

Following gene: 120597902

Centisome position: 25.87

GC content: 46.89

Gene sequence:

>450_bases
ATGGCAAAAGAATTCAGTCGTACACGTCGTATAGCACAGCAACTTCAGCAAGAACTTGCTCAGGTGCTACAACGCGACAT
GAAAGATCCTCGTATTGGCTTTGTTACCGTAAATGACGTAGACGTCTCACGCGATCTCAGCTACGCCAAAGTCTTTGTGA
CCTTCTTTGAAGAAGATAAAGCAGTGGTGCAGGAAAAGCTCAACGCTTTGATCAGCGCCGCCCCCTACATCCGCACCTTA
GTGGCGGGCCGTATGAAGCTGCGTGTTATGCCTGAACTACGTTTTGTTTATGACAGTTCATTGGTTGAGGGGATGCGTAT
GTCTAACCTCGTTAGCCAAGTCATCAACCAAGACAAGGCAAAACAACAGCAGTTTGGTAGCGCAGATGAAGTCCTTAATG
AGGATGAGGGCGCTACCGACGATACAGATGATACAAAAGGTAAAGATTAA

Upstream 100 bases:

>100_bases
AGTATTCGAAACCGTCGAAGTGGCACGTACACTTTAACGATAAGGAGGGCGGCATTAGCCGCCCTTTCGATTTAATGACA
TTAGCTATGGTACAAATATT

Downstream 100 bases:

>100_bases
TGGCTCGTCGTCCGAAAGGCCGTTTTATCGATGGGATAGTGTTACTGGATAAATCCACTGGCATGAGTTCTAACTTTGCC
TTGCAAAGGGTAAAGCGCTT

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MAKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKAVVQEKLNALISAAPYIRTL
VAGRMKLRVMPELRFVYDSSLVEGMRMSNLVSQVINQDKAKQQQFGSADEVLNEDEGATDDTDDTKGKD

Sequences:

>Translated_149_residues
MAKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKAVVQEKLNALISAAPYIRTL
VAGRMKLRVMPELRFVYDSSLVEGMRMSNLVSQVINQDKAKQQQFGSADEVLNEDEGATDDTDDTKGKD
>Mature_148_residues
AKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKAVVQEKLNALISAAPYIRTLV
AGRMKLRVMPELRFVYDSSLVEGMRMSNLVSQVINQDKAKQQQFGSADEVLNEDEGATDDTDDTKGKD

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family

Homologues:

Organism=Escherichia coli, GI1789558, Length=124, Percent_Identity=52.4193548387097, Blast_Score=135, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBFA_SHEPC (A4Y9B9)

Other databases:

- EMBL:   CP000681
- RefSeq:   YP_001184351.1
- ProteinModelPortal:   A4Y9B9
- SMR:   A4Y9B9
- STRING:   A4Y9B9
- GeneID:   5078665
- GenomeReviews:   CP000681_GR
- KEGG:   spc:Sputcn32_2833
- eggNOG:   COG0858
- HOGENOM:   HBG751055
- OMA:   SIEYGNR
- PhylomeDB:   A4Y9B9
- ProtClustDB:   PRK00521
- GO:   GO:0005737
- HAMAP:   MF_00003
- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- Gene3D:   G3DSA:3.30.300.20
- ProDom:   PD007327
- TIGRFAMs:   TIGR00082

Pfam domain/function: PF02033 RBFA; SSF89919 Rib_bd_factA

EC number: NA

Molecular weight: Translated: 16938; Mature: 16807

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCHHHHHHHHHHHHHHH
AVVQEKLNALISAAPYIRTLVAGRMKLRVMPELRFVYDSSLVEGMRMSNLVSQVINQDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQQQFGSADEVLNEDEGATDDTDDTKGKD
HHHHCCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCHHHHHHHHHHHHHHH
AVVQEKLNALISAAPYIRTLVAGRMKLRVMPELRFVYDSSLVEGMRMSNLVSQVINQDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQQQFGSADEVLNEDEGATDDTDDTKGKD
HHHHCCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA