Click here to switch to the map view.

The map label for this gene is rlmI [H]

Identifier: 120597842

GI number: 120597842

Start: 1158123

End: 1159313

Strand: Direct

Name: rlmI [H]

Synonym: Sputw3181_1012

Alternate gene names: 120597842

Gene position: 1158123-1159313 (Clockwise)

Preceding gene: 120597841

Following gene: 120597844

Centisome position: 24.6

GC content: 49.12

Gene sequence:

>1191_bases
ATGGCTATCAGAATCAAACTTAAACCCGGCCGCGAGAAGTCGCTCGAGCGTCGCCATCCTTGGGTATTTTCCAATGCTAT
TCATAACATTAAGGGAAAACCTGAAGCGGGTGAGACAGTCGATGTGGTCGCGCATGATGGTCATTGGTTAGGTCGTGGTG
CTTGGTCGCCTGAGTCGCAAATTCAAGTGCGGATTTGGACTTTTGATCGCGAAGAAGAAATTGATCGCGCGTTTTTTGCC
AGACGTTTACAGCGGGCGCAAATCGGCCGTAACGATCTAATCCGCGAGCAGGGTTTAACGGGTTACCGTTTAGTGGCCGC
CGAGTCCGATGGTTTGCCTGGGATCACCATCGACAGATATGCCAATGTGCTGGTGTGCCAGTTACTGAGCACAGGTGCTG
ATTTATGGCGCGATACCTTAGTCGAGTTATTGGCTGAGCAATATCCAGATTGTGCTATCTATGAGCGTTCTGATGTGGAT
TCCCGTAAGAAAGAAGGTCTGCTGCCTGTGACGGGTTTACTGCACGGCACTCTGCCAGAAATGCCAGTGATTATCGAAGA
AAACGGCATCAAGATCGCCGTCGATGTGATTAAAGGCCATAAGACAGGTTTCTATCTCGATCAACGTGATAACCGCGCCA
TTGCCGCCCGTTTTGTCAAAGATAAATCGGTATTGAACTGTTTTTGCTACACGGGGACTTTTGGTCTGTACGCTGCGAAA
GCAGGTGCTGCCAGTATTGAAAACGTCGATGTGTCTTCTCTAGCCTTGGCGACAGCGCGCCTCAATATGCAAGTGAACGG
TTTGAGTGATGACAATGTGCATTACAACGAAGCCGATGTGTTCAAGTTGCTGCGCCAGTACCGTGATGAAGGCAAAACCT
TTGATGTGATTGTGCTCGACCCGCCTAAGTTTGCCGATAACAAAGCGCAGTTAAACGGGGCTTGCCGTGGTTATAAAGAC
ATCAACATGATTGCATTACAGTTGTTGAATCCGGGCGGTGTGTTATTGACCTTCTCTTGCTCAGGATTAATGCCTGCGGA
TCTGTTCCAAAAAATCGTGGCCGATGCCGCGCTCGATGCGAAACGTGAAATCCAGTTTATTGAACGCTTAAGCCAAGCGA
GCGATCACCCTATCGGTAGCGCCTTCCCAGAAGGATTCTACTTAAAAGGTCTAGTTGCCAGAGCTTGGTAA

Upstream 100 bases:

>100_bases
TGTTCGGTACTCGCACTTAGGTGGCATTGGGTATATCATCCGTTTCCCATTTTTAGACAGCAGCCCTTTTTTAGTGGCGG
CCCTATTTTTAAAGGCAACT

Downstream 100 bases:

>100_bases
GCCAAGCGTTTGATATAAGCTGTCTGCGATGGGCAACTTAGTTAGATAACATTGAGCTATAAAAAGAATCATAAAAAAAT
GCCAGCTCTTGAATAAAGTG

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDRAFFA
RRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRYANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVD
SRKKEGLLPVTGLLHGTLPEMPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKD
INMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW

Sequences:

>Translated_396_residues
MAIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDRAFFA
RRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRYANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVD
SRKKEGLLPVTGLLHGTLPEMPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKD
INMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW
>Mature_395_residues
AIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDRAFFAR
RLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRYANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVDS
RKKEGLLPVTGLLHGTLPEMPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAKA
GAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKDI
NMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=394, Percent_Identity=58.3756345177665, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1787181, Length=238, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44101; Mature: 43970

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50890 PUA ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQ
CEEEEEECCCCCHHHHHCCCEEEHHHHHCCCCCCCCCCEEEEEEECCEEECCCCCCCCCC
IQVRIWTFDREEEIDRAFFARRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRY
EEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCEEHHHH
ANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVDSRKKEGLLPVTGLLHGTLPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCHHHHHHCCCCC
MPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
CCEEEECCCCEEEEEEEECCCCCEEEECCCCCEEEHHHHHCCCCEEEEEECCCCHHHHHC
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRQYRDEGKTFDVIVLD
CCCCCCCCCCHHHHEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PPKFADNKAQLNGACRGYKDINMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCH
KREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCC
>Mature Secondary Structure 
AIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQ
EEEEEECCCCCHHHHHCCCEEEHHHHHCCCCCCCCCCEEEEEEECCEEECCCCCCCCCC
IQVRIWTFDREEEIDRAFFARRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRY
EEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCEEHHHH
ANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVDSRKKEGLLPVTGLLHGTLPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCHHHHHHCCCCC
MPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
CCEEEECCCCEEEEEEEECCCCCEEEECCCCCEEEHHHHHCCCCEEEEEECCCCHHHHHC
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRQYRDEGKTFDVIVLD
CCCCCCCCCCHHHHEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PPKFADNKAQLNGACRGYKDINMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCH
KREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA