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The map label for this gene is btuF [H]

Identifier: 120597825

GI number: 120597825

Start: 1136255

End: 1137082

Strand: Direct

Name: btuF [H]

Synonym: Sputw3181_0995

Alternate gene names: 120597825

Gene position: 1136255-1137082 (Clockwise)

Preceding gene: 120597824

Following gene: 120597828

Centisome position: 24.13

GC content: 45.53

Gene sequence:

>828_bases
ATGTATATACAAGGCGTTATTGGGCTGTTTTTCTGTCTATATCTGTTGTGTTCGACTGATGTACTGGCGCAGCCCGCTAA
ACGCATTATTGCGCTCTCTCCCCATGCGGTGGAAATGCTTTATGCCATAGGCGCGGGCGATACGATTGTGGCCGCAACCG
ATTATGCGGATTATCCTGAGGCTGCAAAAAAGATCCCGCGTATTGGCGGTTACTATGGCATTCAAATGGAGCGGGTACTA
GAGCTTAATCCCGATCTGATTGTGGTGTGGGATTCGGGTAATAAAGCCGAAGATATCAACCAGCTTAGGACGTTAGGATT
TAATCTTTATGGTAGCGATCCTAAAACCTTAGAAGGCGTCGCCAAGGAGCTTGAAGAGTTAGGCCAACTTACGGGGCATG
TTGAAGAAGCAAGCAAGGCTGCGGCCGCTTATCGCGCTGAGCTTATCCGATTGCGGGTTGAAAATGCTAAAAAATCAGAA
CCTAAGGTATTTTATCAACTCTGGTCTACCCCCTTAATGACAGTCTCTAAAAACAGTTGGATCCAAGAGATTATGAGTGT
GTGCCATGGACAAAATGTGTTTTACGATGCCGATAGCGATTATCCGCAGGTGAGTTTAGAAAATGTGTTACTGACTTTAC
CAGATGTGATTTTACAAAGTGAAGAGGAAGGCAATGTGAAAGGGGTTGATTGGCGCCAATGGCCAGAAATTCCTGCCGTT
AAAAATCAGCATATTTATCAACTCAATGCCGATTTATTGCACCGTGCGACGCCCAGAGCATTACTTGGCGTTCAAGCCTT
ATGTGATGCCTTGGATAAAGCACGTTAA

Upstream 100 bases:

>100_bases
TGGGAGCGAGACCCCCAATATCGGCCAAAAAATCTAAAAGTTTACTTGGATCGCTATGGCTGGCCGACTGAGCTAATCAA
TTAACCACTTAAGGAATAAT

Downstream 100 bases:

>100_bases
ATAAAACGCCTGACAGATTATTCCGTCAGGCGTTTAGTCTGATGCCACTATTTTCTAAGCGTTTAGAAAGGTGCGTTGAT
ATCAACCACATTAATCAGCT

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MYIQGVIGLFFCLYLLCSTDVLAQPAKRIIALSPHAVEMLYAIGAGDTIVAATDYADYPEAAKKIPRIGGYYGIQMERVL
ELNPDLIVVWDSGNKAEDINQLRTLGFNLYGSDPKTLEGVAKELEELGQLTGHVEEASKAAAAYRAELIRLRVENAKKSE
PKVFYQLWSTPLMTVSKNSWIQEIMSVCHGQNVFYDADSDYPQVSLENVLLTLPDVILQSEEEGNVKGVDWRQWPEIPAV
KNQHIYQLNADLLHRATPRALLGVQALCDALDKAR

Sequences:

>Translated_275_residues
MYIQGVIGLFFCLYLLCSTDVLAQPAKRIIALSPHAVEMLYAIGAGDTIVAATDYADYPEAAKKIPRIGGYYGIQMERVL
ELNPDLIVVWDSGNKAEDINQLRTLGFNLYGSDPKTLEGVAKELEELGQLTGHVEEASKAAAAYRAELIRLRVENAKKSE
PKVFYQLWSTPLMTVSKNSWIQEIMSVCHGQNVFYDADSDYPQVSLENVLLTLPDVILQSEEEGNVKGVDWRQWPEIPAV
KNQHIYQLNADLLHRATPRALLGVQALCDALDKAR
>Mature_275_residues
MYIQGVIGLFFCLYLLCSTDVLAQPAKRIIALSPHAVEMLYAIGAGDTIVAATDYADYPEAAKKIPRIGGYYGIQMERVL
ELNPDLIVVWDSGNKAEDINQLRTLGFNLYGSDPKTLEGVAKELEELGQLTGHVEEASKAAAAYRAELIRLRVENAKKSE
PKVFYQLWSTPLMTVSKNSWIQEIMSVCHGQNVFYDADSDYPQVSLENVLLTLPDVILQSEEEGNVKGVDWRQWPEIPAV
KNQHIYQLNADLLHRATPRALLGVQALCDALDKAR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=250, Percent_Identity=32.4, Blast_Score=148, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30651; Mature: 30651

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIQGVIGLFFCLYLLCSTDVLAQPAKRIIALSPHAVEMLYAIGAGDTIVAATDYADYPE
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCHHHHHHHHCCCCCEEEEECCCCCCCH
AAKKIPRIGGYYGIQMERVLELNPDLIVVWDSGNKAEDINQLRTLGFNLYGSDPKTLEGV
HHHHCCCCCCEECHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCHHHHHH
AKELEELGQLTGHVEEASKAAAAYRAELIRLRVENAKKSEPKVFYQLWSTPLMTVSKNSW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCHHH
IQEIMSVCHGQNVFYDADSDYPQVSLENVLLTLPDVILQSEEEGNVKGVDWRQWPEIPAV
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
KNQHIYQLNADLLHRATPRALLGVQALCDALDKAR
CCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYIQGVIGLFFCLYLLCSTDVLAQPAKRIIALSPHAVEMLYAIGAGDTIVAATDYADYPE
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCHHHHHHHHCCCCCEEEEECCCCCCCH
AAKKIPRIGGYYGIQMERVLELNPDLIVVWDSGNKAEDINQLRTLGFNLYGSDPKTLEGV
HHHHCCCCCCEECHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCHHHHHH
AKELEELGQLTGHVEEASKAAAAYRAELIRLRVENAKKSEPKVFYQLWSTPLMTVSKNSW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCHHH
IQEIMSVCHGQNVFYDADSDYPQVSLENVLLTLPDVILQSEEEGNVKGVDWRQWPEIPAV
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
KNQHIYQLNADLLHRATPRALLGVQALCDALDKAR
CCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA