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The map label for this gene is pitA [H]

Identifier: 120597757

GI number: 120597757

Start: 1052749

End: 1054218

Strand: Direct

Name: pitA [H]

Synonym: Sputw3181_0927

Alternate gene names: 120597757

Gene position: 1052749-1054218 (Clockwise)

Preceding gene: 120597756

Following gene: 120597759

Centisome position: 22.36

GC content: 45.03

Gene sequence:

>1470_bases
ATGTTCGAAATATTTACGACGTTAGGTTTTGGATGGTCTGTTGGTTTAGTGTTAGCGGTGCTGTTTGTACTTGCATATGA
ATTTATCAATGGCTTTCACGACACGGCCAATGCGGTGGCAACGGTTATCTATACTAAAGCGATGCCAGCTAATCTTGCAG
TTGTAACCTCTGCGCTATTTAATTTTGCGGGCGTATTATTAGGTGGCTTAGGTGTAGCTTATGCGATAGTGCATTTGTTA
CCTGTCGATTCCTTACTAGGCATGGATTCTACCCAAGGGTTATTAATGGTGTTTTCCCTCTTAGTTTCGGCGATTATTTG
GAACTTAGGCACTTGGTATTTTGGCATTCCTGCATCAAGTTCGCATACCTTAATAGGCTCGATAATGGGAGTGGGTGGAG
CATTTGCATGGCTACACGATCAACCGATCTTACATGGCATAAACACCACTAAAGCCACGCAGATTATGCTGTCTCTGATC
ATTTCTCCGACATTAGGTTTTGTGATGGCAGCGTTTTTTTTACTGCTGATGAAATCGATTTGGCAAAAACATAAAATCCA
CCGAACACCCGATGAACAAATCCAAATCAATGGCAAAAAGCATCCACCATTTTGGGCGAGGGTCAGCTTAATTGTATCTG
CCATGGGGGTCAGTTTTGCCCATGGTTCTAACGATGGTCAAAAGGGGATTGGCCTAGTCATGCTCGTATTGATCTGTATG
GCTCCTGCATATTTTGCCCTCAATATGAATAGTCAGCCCTATGACTTAGAAAGAACGCAGGATGCGAATCGGCGTATTAT
GGCTATTTATGATCGTAATCAGACCGCTATTGGTGAGCTGGTTGATTTTAAGGCTGATATCCATGTTGCCGATAATATGT
TGACTCATTGCTCGGCAGAGACAGTGTTACCTGCGATGTCACAACTCGATCGTCATTTGGGCAAAGTATCCACTTTTAAA
GAGATGGGGGTTGAAGAGCGCCGTGAAGTACGCCGTTTATTGCTCTGTATCGACGATACGGCCCGTAAAGTATCCAAGCT
CGAACTGCCTAAAAAAGAATTAAGTTCACTCGCCAAGTGGCGTAAAGATCTGACTATCATCACTGAATATGCTCCCTTGT
GGGTCATTATCGCGATTGCAACCGCATTGGGTTGTGGCACCTTAGTCGGTTGGCGCCGAATTGTGTATACCGTGGGTGAA
AAGATTGGTTCATCTGGCATGACCTATAGCCAAGGTATCGCTGCGCAGGTCACGGCCGCGGCATCCATCGGGGTGGCGAG
TCTGACTGGTATGCCTGTTTCTACGACCCATATTTTATCTTCCGCAGTAGCGGGCACTATGGTGGCGAATCGTTCGGGCT
TACAAAGCCAGACAATTAAGCAGATCATGCTTGCTTGGTTACTTACCCTGCCGATTACCATTCTACTTTCTGGTGGTATG
TTTATTTTAGCTAATGCAATGTTTGGCTAA

Upstream 100 bases:

>100_bases
GAGGACTCATTCACTTTTTGACAATCTAAATAATTTTTCACTGTAAAAGACCCCAAACTTGGGCGTAATCTGTGGCGTCC
TTGTCATAGGTTAGCTATTT

Downstream 100 bases:

>100_bases
TGCAATAATTGGATTGTGCGCAGCAGCTTGACGATAAATTTCGCAGTATTTAGTGCAGCATTGAGTACCGTTTATGTTGA
ATAACGGTACTGCACGGACG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MFEIFTTLGFGWSVGLVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLVSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWLHDQPILHGINTTKATQIMLSLI
ISPTLGFVMAAFFLLLMKSIWQKHKIHRTPDEQIQINGKKHPPFWARVSLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALNMNSQPYDLERTQDANRRIMAIYDRNQTAIGELVDFKADIHVADNMLTHCSAETVLPAMSQLDRHLGKVSTFK
EMGVEERREVRRLLLCIDDTARKVSKLELPKKELSSLAKWRKDLTIITEYAPLWVIIAIATALGCGTLVGWRRIVYTVGE
KIGSSGMTYSQGIAAQVTAAASIGVASLTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQIMLAWLLTLPITILLSGGM
FILANAMFG

Sequences:

>Translated_489_residues
MFEIFTTLGFGWSVGLVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLVSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWLHDQPILHGINTTKATQIMLSLI
ISPTLGFVMAAFFLLLMKSIWQKHKIHRTPDEQIQINGKKHPPFWARVSLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALNMNSQPYDLERTQDANRRIMAIYDRNQTAIGELVDFKADIHVADNMLTHCSAETVLPAMSQLDRHLGKVSTFK
EMGVEERREVRRLLLCIDDTARKVSKLELPKKELSSLAKWRKDLTIITEYAPLWVIIAIATALGCGTLVGWRRIVYTVGE
KIGSSGMTYSQGIAAQVTAAASIGVASLTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQIMLAWLLTLPITILLSGGM
FILANAMFG
>Mature_489_residues
MFEIFTTLGFGWSVGLVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLVSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWLHDQPILHGINTTKATQIMLSLI
ISPTLGFVMAAFFLLLMKSIWQKHKIHRTPDEQIQINGKKHPPFWARVSLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALNMNSQPYDLERTQDANRRIMAIYDRNQTAIGELVDFKADIHVADNMLTHCSAETVLPAMSQLDRHLGKVSTFK
EMGVEERREVRRLLLCIDDTARKVSKLELPKKELSSLAKWRKDLTIITEYAPLWVIIAIATALGCGTLVGWRRIVYTVGE
KIGSSGMTYSQGIAAQVTAAASIGVASLTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQIMLAWLLTLPITILLSGGM
FILANAMFG

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=470, Percent_Identity=50.8510638297872, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789360, Length=470, Percent_Identity=51.4893617021277, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 52962; Mature: 52962

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCC
>Mature Secondary Structure
MFEIFTTLGFGWSVGLVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALF
CCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NFAGVLLGGLGVAYAIVHLLPVDSLLGMDSTQGLLMVFSLLVSAIIWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGSIMGVGGAFAWLHDQPILHGINTTKATQIMLSLIISPTLGFVMAAFFLLLMKSI
CCHHHHHHHHCCCHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKHKIHRTPDEQIQINGKKHPPFWARVSLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
HHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
APAYFALNMNSQPYDLERTQDANRRIMAIYDRNQTAIGELVDFKADIHVADNMLTHCSAE
CCHHEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHCCHH
TVLPAMSQLDRHLGKVSTFKEMGVEERREVRRLLLCIDDTARKVSKLELPKKELSSLAKW
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RKDLTIITEYAPLWVIIAIATALGCGTLVGWRRIVYTVGEKIGSSGMTYSQGIAAQVTAA
HHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHH
ASIGVASLTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQIMLAWLLTLPITILLSGGM
HHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
FILANAMFG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]