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The map label for this gene is cysH [H]

Identifier: 120597713

GI number: 120597713

Start: 1003034

End: 1003816

Strand: Direct

Name: cysH [H]

Synonym: Sputw3181_0883

Alternate gene names: 120597713

Gene position: 1003034-1003816 (Clockwise)

Preceding gene: 120597712

Following gene: 120597714

Centisome position: 21.3

GC content: 48.4

Gene sequence:

>783_bases
ATGGCTGATCTTGAGCATAAAACCAGTGATACAGTGCCGACAGTGGTCAGCGCTGCCGAACTCAAGGCATTATTGAGTGC
GCCTAAGGCTGAGCAAGCGATAGAGCTTGAACGTATTAATCAGTTCTTAGGTGGATTGAGCGCGCAAGAGCGAGTGCTAT
GGGGCTTAACTTATTTGCCCGGTAATCATGCTCTGTCCTCAAGTTTTGGGATCCAAGCCGCTGTAATGCTGCATATGGTG
AGTCAGGCACAGGCGAATATTCCGGTTATCCTCACGGATACGGGTTATTTATTCCCTGAGACTTATCAGTTTATCGACGA
GCTGACAGAGCGTTTATCGCTGAATTTAAAAGTGTACCAAGCAGTGATGACATCGGCATGGCAAGAGGCACGCTTTGGTC
AGTTGTGGGAGCAAGGGTTAGACGGGCTAGAGCAATACAACCGTTTAAACAAAGTTGAGCCTATGCAACGCGCCTTAGCC
GAGCTTGAAGTCGGCACTTGGTTTGCGGGGCTTAGACGCAGTCAAGCCAGTACCCGTGAGGCGCTGCCGATATTAGCTAT
CCATGGCACACGCTTTAAGTTACTGCCAATTATCGAATGGTCGAATAAAGATGTGCACCTCTATTTGACGCAATTTGACT
TACCGTACCATCCACTTTGGGAGCAAGGCTATGTTTCTGTGGGGGATACCCATTCGAGTAAACCACTCGAATTAGGCATG
ACGGAGGAAGAAACCCGCTTTAATGGCCTAAAACGTGAGTGTGGTCTGCACTACGAAATCTAA

Upstream 100 bases:

>100_bases
GGCCGCTACGCCGTTGAGCGTGATGCTGGCGAAACCTTTGGTAACTTCACTGTCCGCGTGGGCGTAGTGAAGGCGGTGAT
CGATGCCGCTAAGGATTTTC

Downstream 100 bases:

>100_bases
CTATCTTATTGAGTTAAAAACTTAACACCTCGATTTTGCTCGGGGTGTTTTTATTTATGGTCTTTTATTGTTTGATTTTG
AAGCGAATTAATAGTTACAA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MADLEHKTSDTVPTVVSAAELKALLSAPKAEQAIELERINQFLGGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLHMV
SQAQANIPVILTDTGYLFPETYQFIDELTERLSLNLKVYQAVMTSAWQEARFGQLWEQGLDGLEQYNRLNKVEPMQRALA
ELEVGTWFAGLRRSQASTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWEQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYEI

Sequences:

>Translated_260_residues
MADLEHKTSDTVPTVVSAAELKALLSAPKAEQAIELERINQFLGGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLHMV
SQAQANIPVILTDTGYLFPETYQFIDELTERLSLNLKVYQAVMTSAWQEARFGQLWEQGLDGLEQYNRLNKVEPMQRALA
ELEVGTWFAGLRRSQASTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWEQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYEI
>Mature_259_residues
ADLEHKTSDTVPTVVSAAELKALLSAPKAEQAIELERINQFLGGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLHMVS
QAQANIPVILTDTGYLFPETYQFIDELTERLSLNLKVYQAVMTSAWQEARFGQLWEQGLDGLEQYNRLNKVEPMQRALAE
LEVGTWFAGLRRSQASTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWEQGYVSVGDTHSSKPLELGMT
EEETRFNGLKRECGLHYEI

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=242, Percent_Identity=60.7438016528926, Blast_Score=313, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6325425, Length=241, Percent_Identity=31.5352697095436, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29359; Mature: 29228

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLEHKTSDTVPTVVSAAELKALLSAPKAEQAIELERINQFLGGLSAQERVLWGLTYLP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHEEEEECC
GNHALSSSFGIQAAVMLHMVSQAQANIPVILTDTGYLFPETYQFIDELTERLSLNLKVYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHH
AVMTSAWQEARFGQLWEQGLDGLEQYNRLNKVEPMQRALAELEVGTWFAGLRRSQASTRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWEQGYVSVGDTHSSKPLELGM
CCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEECC
TEEETRFNGLKRECGLHYEI
CCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADLEHKTSDTVPTVVSAAELKALLSAPKAEQAIELERINQFLGGLSAQERVLWGLTYLP
CCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHEEEEECC
GNHALSSSFGIQAAVMLHMVSQAQANIPVILTDTGYLFPETYQFIDELTERLSLNLKVYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHH
AVMTSAWQEARFGQLWEQGLDGLEQYNRLNKVEPMQRALAELEVGTWFAGLRRSQASTRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWEQGYVSVGDTHSSKPLELGM
CCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEECC
TEEETRFNGLKRECGLHYEI
CCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]