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The map label for this gene is cysI [H]

Identifier: 120597712

GI number: 120597712

Start: 1001344

End: 1003041

Strand: Direct

Name: cysI [H]

Synonym: Sputw3181_0882

Alternate gene names: 120597712

Gene position: 1001344-1003041 (Clockwise)

Preceding gene: 120597711

Following gene: 120597713

Centisome position: 21.27

GC content: 50.24

Gene sequence:

>1698_bases
ATGAGTGAGCAAAAGTTAGCGTTAAACGAATACCTAAAGACCGACAGTGATTACCTGCGCGGGACCATCAAAGAAGGTTT
AGATTCTTCTGTGACAGGCAGTTTCAGCGATGGCGATCAGCAGCTGATCAAATTTCATGGCTTCTACCAGCAGGATGACC
GCGATTTACGTAACGAACGTAAAGAGCAAAAACTCGAGCCTTTATATAGCTTTATGTTGCGTGCCCGCGTACCTGGTGGT
ATTTGTACGCCGCAGCAATGGTTAGGTGTGGACAAAATTGCCTCGACCTTAACCAGCTCGAACAGCATTCGTTTGACGAC
CCGTCAAACCTTCCAATACCACGGTATTCCTAAGCGTAACTTGAAGACCATTATTCAAGACTTAGATCGGCAGGCGCTGG
ATTCGATTGCTGCCTGTGGTGACGTTAACCGTAACGTCATGTGTAACCCCAATCCGGTGGAATCTAAACTGCATGAGCAG
GCCTATGCGGTGGCGAAAAAGCTCTCAGATCATCTGCTGCCACACACTCGCGCCTACGCCGAAATCTGGTTAGATGAAGA
AAAGTTACTGACCACGGAAGATGAAACCGTTGAACCTGTGTATGGCAAAACCTATTTGCCACGTAAGTTTAAGATGGCGG
TTGCGGTGCCTCCCGATAACGATGTTGACGTTTACACGAACGATTTAGGTTTTATCGCCGTGGCCGAAAATGGCGAGCTG
GTTGGCTTTAACCTGACTGCGGGTGGCGGCATGGGCTCGACCCATGGCGAAGTGGAAACTTTCCCACGTCTGGCTGACGA
CTTTGGTTTTATCAAAACTGAAGATGTGATGAAATTTGCCGAAGCTGTGATGACAGTGCAACGCGACTGGGGTAATCGCT
CTAATCGTAAGCGCTCGCGCCTTAAATACACTATTGTCGACCATGGCTATGAGAAGTTTAAAGCCGAAGTCGAGGCCCGT
GCTGGGGTGAAATTTGAACCTAAGCGCGATGTAGTGATTGGCGATCGCGGCGATCGTTATGGTTGGGTCGAAGGCGTTGA
CGGTAAGTGGCATTTAACCTTATTTATCGAAAGCGGTCGTATTAAAGATTTACCGGGGCAAACGTTGCAAACGGGGCTGC
GTGAAATTGCTAAGATCCACAAGGGCGATTTCCGCATGACCTCGAATCAAAACATGATCATCGCAGGCGTCGCGGCGGAA
GATAAAGCCACCATTGAAGGCTTAGCCCGCAAACACGGTCTACTTGGCCAAGTGTTGACACAAACCCGCGGTCACTCTAT
TGCCTGTGTGGCGTTGCCGACGTGTCCATTAGCGATGGCGGAAGCCGAGCGTTATTTCCCTGAGTTTATCGATCACATCG
ATGCGCTGCAGGCAAAACACGGCATTAGCGAGCAAGCGATTGTGGTGCGTATGACGGGTTGTCCTAACGGTTGTGCCCGT
CCATTTGCCGCTGAAATTGGCCTTGTGGGTAAGGCGCCGGGTCGTTATAACTTGTATTTAGGTGCAAGTTTTGAAGGGAC
TCGCCTGAACAAGATGCACAGAGAAAATATCCAAGAGGCGGACATTCTCGCCGAATTGGATACCCTGTTTGGCCGCTACG
CCGTTGAGCGTGATGCTGGCGAAACCTTTGGTAACTTCACTGTCCGCGTGGGCGTAGTGAAGGCGGTGATCGATGCCGCT
AAGGATTTTCATGGCTGA

Upstream 100 bases:

>100_bases
ATCTCAACCGTTGCGCAGATAAGAAACGCATAAGAATAGCCAAAGCTGCACACGTGATGACCTGTGCAGCCAGCGAACCC
AATTTTAAGAGGCAGTTGCC

Downstream 100 bases:

>100_bases
TCTTGAGCATAAAACCAGTGATACAGTGCCGACAGTGGTCAGCGCTGCCGAACTCAAGGCATTATTGAGTGCGCCTAAGG
CTGAGCAAGCGATAGAGCTT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVPGG
ICTPQQWLGVDKIASTLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQDLDRQALDSIAACGDVNRNVMCNPNPVESKLHEQ
AYAVAKKLSDHLLPHTRAYAEIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRSNRKRSRLKYTIVDHGYEKFKAEVEAR
AGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGRIKDLPGQTLQTGLREIAKIHKGDFRMTSNQNMIIAGVAAE
DKATIEGLARKHGLLGQVLTQTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKHGISEQAIVVRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKMHRENIQEADILAELDTLFGRYAVERDAGETFGNFTVRVGVVKAVIDAA
KDFHG

Sequences:

>Translated_565_residues
MSEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVPGG
ICTPQQWLGVDKIASTLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQDLDRQALDSIAACGDVNRNVMCNPNPVESKLHEQ
AYAVAKKLSDHLLPHTRAYAEIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRSNRKRSRLKYTIVDHGYEKFKAEVEAR
AGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGRIKDLPGQTLQTGLREIAKIHKGDFRMTSNQNMIIAGVAAE
DKATIEGLARKHGLLGQVLTQTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKHGISEQAIVVRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKMHRENIQEADILAELDTLFGRYAVERDAGETFGNFTVRVGVVKAVIDAA
KDFHG
>Mature_564_residues
SEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVPGGI
CTPQQWLGVDKIASTLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQDLDRQALDSIAACGDVNRNVMCNPNPVESKLHEQA
YAVAKKLSDHLLPHTRAYAEIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGELV
GFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRSNRKRSRLKYTIVDHGYEKFKAEVEARA
GVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGRIKDLPGQTLQTGLREIAKIHKGDFRMTSNQNMIIAGVAAED
KATIEGLARKHGLLGQVLTQTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKHGISEQAIVVRMTGCPNGCARP
FAAEIGLVGKAPGRYNLYLGASFEGTRLNKMHRENIQEADILAELDTLFGRYAVERDAGETFGNFTVRVGVVKAVIDAAK
DFHG

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=561, Percent_Identity=58.2887700534759, Blast_Score=694, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=593, Percent_Identity=43.8448566610455, Blast_Score=528, Evalue=1e-150,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 62997; Mature: 62866

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNER
CCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVPGGICTPQQWLGVDKIASTLTSSNSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEEEEEEECCCCHHH
LKTIIQDLDRQALDSIAACGDVNRNVMCNPNPVESKLHEQAYAVAKKLSDHLLPHTRAYA
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
HHEECCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCE
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRSNRKRSR
EEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LKYTIVDHGYEKFKAEVEARAGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGR
EEEEEECCCHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCEECCCCEEEEEEEEECCC
IKDLPGQTLQTGLREIAKIHKGDFRMTSNQNMIIAGVAAEDKATIEGLARKHGLLGQVLT
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
QTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKHGISEQAIVVRMTGCPNGCAR
HCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKMHRENIQEADILAELDTLFGRYAVERDAG
CHHHHCCCEECCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
ETFGNFTVRVGVVKAVIDAAKDFHG
CCCCCEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNER
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVPGGICTPQQWLGVDKIASTLTSSNSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEEEEEEECCCCHHH
LKTIIQDLDRQALDSIAACGDVNRNVMCNPNPVESKLHEQAYAVAKKLSDHLLPHTRAYA
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
HHEECCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCE
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRSNRKRSR
EEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LKYTIVDHGYEKFKAEVEARAGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGR
EEEEEECCCHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCEECCCCEEEEEEEEECCC
IKDLPGQTLQTGLREIAKIHKGDFRMTSNQNMIIAGVAAEDKATIEGLARKHGLLGQVLT
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
QTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKHGISEQAIVVRMTGCPNGCAR
HCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKMHRENIQEADILAELDTLFGRYAVERDAG
CHHHHCCCEECCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
ETFGNFTVRVGVVKAVIDAAKDFHG
CCCCCEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA