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The map label for this gene is cysJ [H]

Identifier: 120597711

GI number: 120597711

Start: 999445

End: 1001244

Strand: Direct

Name: cysJ [H]

Synonym: Sputw3181_0881

Alternate gene names: 120597711

Gene position: 999445-1001244 (Clockwise)

Preceding gene: 120597708

Following gene: 120597712

Centisome position: 21.23

GC content: 49.11

Gene sequence:

>1800_bases
ATGTTGTTAAAAGAACTTTCATCGCTAGCTTCGCCATTGTCTCAAGGACAAGTCGAGAAGCTAAAACAACTCACTTCTGA
GTTAAGTGCAGTGCAACTGGCTTGGGTGAGTGGCTATCTTGCTGCAACTGCCAATGCGGGTCAATTAGCGCCTGTTGCGC
AGGCGCAAACGGCTCAAACAGTGACGATTCTCTATGGCAGCCAAACGGGTAATGGCCGTGGTGTCGCCAAAGCGCTCGCC
GATAAGGCGCAGGCGCAAGGTTATGCGGTCAATCTGGCTTCAATGGGCGAATACAATGTTCGCCAATTAAAGCAAGAAGC
TGTGCTTTTGCTGGTGGTCAGCACCCATGGTGAAGGTGAAGCGCCGGATGATGCGATTGAATTACACAAGTTTTTAGCAT
CGAAACGTGCCCCTAAGTTAGACAATCTACATTACTCAGTGCTCGCGCTGGGGGATTCTAGCTATGAGTTCTTCTGCCAA
ACAGGTAAAGACTTCGATGCCCGCTTAGCAGTACTGGGTGCTAAGTCACTGTTACCTTTGATTGAATGCGATGTGGATTA
TGAAGCTGCCGCGGGTCAGTGGCACGCCGATGTATTAGAGGCCGTTAAGCCGCTGATCGAAACCTCGTCCGCCAGTGTGG
TATCCATCGGCACGGCCAAAGCGATTGGCGAAAGTGAATTTACTAAGCAAAACCCCTATAGCGCCGAAGTGCTTGTGAGC
CAGAAAATTACTGGCCGTGGATCAGACAGAGATGTACGCCATGTTGAAATTGACCTTGGCGACTCAGGGTTAACCTATCA
AGCGGGTGATGCCCTCGGTGTGTGGTTTAGTAATAACGAAGCCTTAGTTGAGGAGATTTTAACTGCACTGTCACTCTCTG
GTGATGAGGAAGTGGTTGTCGAAAAAGAATCTTTGACCCTTAAGCAAGTCTTGGTCGATAGGAAAGAGCTGACTCAGTTA
TATCCTGGATTAGTCAAAGCTTGGGCCGAATTGAGCGGCAGCGCTGAGCTGCTGGCATTGAGCGAAGATAAAGAACAAGT
GCGCCACTTTATCCTTAAGCATCAATTTGCCGATTTAGTCACTCAGTATCCGTTATCAAATAACTCAGTAACCTTAAATG
CAGCAAAACTGCTTGAACTACTGCGTCCATTAACGCCAAGACTTTACTCTATCGCCTCAAGTCAAAGCGAAGTTGAAACA
GAAGTCCACTTAACGGTGGCCTTAGTGGAAGATGAGCGCCACGGCTCAGCACGTTTTGGCGGCGCGTCACACTTCTTAGC
CTCAGCGCAGGAAGGCACGCAAGTTAAGGTTTATGTTGAGCCTAATAAGCACTTCCGTTTACCCGAAAACCCAGAAACAC
CGGTGATCATGATTGGACCTGGCACGGGGGTTGCGCCGTTCCGTGCCTTTATGCAGGAGCGCGTAGCACAAGGGATCCAA
GGTGATAGCTGGTTATTCTTCGGTAATCCACACTTTGAGCAGGATTTCCTGTATCAAACCGAGTGGCAACAGTATTTGAA
AAATGGCGATCTCAGCCGTATCGATGTGGCATTCTCCCGCGATCAGGCCCATAAGATTTATGTGCAGCACCGCATTAAAG
AACAAGGCCAAGCCCTGTGGCAATGGTTGCAAAACGGCGCACATCTCTACATTTGCGGTGATGCCGAGCGGATGGCAAAG
GATGTTCACCAAGCCTTGATTGAGGTGGCGGTTGAAGTCGGTGGTTTGAATATTGAGGCTGCAGAAGCCTACTTTGAAAC
CCTGCGCAGCGATAAACGCTACCAAAAAGACGTGTATTAA

Upstream 100 bases:

>100_bases
TGTATATTCCAAAATGGAATTAAAAATCAAATTTTATTCTTTTTTGCTTTTCTTAAACCACGATAAGCTGGTGAACATTA
CACTGTCATAGGGCCGTCTC

Downstream 100 bases:

>100_bases
TCTCAACCGTTGCGCAGATAAGAAACGCATAAGAATAGCCAAAGCTGCACACGTGATGACCTGTGCAGCCAGCGAACCCA
ATTTTAAGAGGCAGTTGCCA

Product: sulfite reductase (NADPH) flavoprotein, alpha chain

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPVAQAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQEAVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFDARLAVLGAKSLLPLIECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLVS
QKITGRGSDRDVRHVEIDLGDSGLTYQAGDALGVWFSNNEALVEEILTALSLSGDEEVVVEKESLTLKQVLVDRKELTQL
YPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLVTQYPLSNNSVTLNAAKLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGSARFGGASHFLASAQEGTQVKVYVEPNKHFRLPENPETPVIMIGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKIYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLNIEAAEAYFETLRSDKRYQKDVY

Sequences:

>Translated_599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPVAQAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQEAVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFDARLAVLGAKSLLPLIECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLVS
QKITGRGSDRDVRHVEIDLGDSGLTYQAGDALGVWFSNNEALVEEILTALSLSGDEEVVVEKESLTLKQVLVDRKELTQL
YPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLVTQYPLSNNSVTLNAAKLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGSARFGGASHFLASAQEGTQVKVYVEPNKHFRLPENPETPVIMIGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKIYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLNIEAAEAYFETLRSDKRYQKDVY
>Mature_599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPVAQAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQEAVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFDARLAVLGAKSLLPLIECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLVS
QKITGRGSDRDVRHVEIDLGDSGLTYQAGDALGVWFSNNEALVEEILTALSLSGDEEVVVEKESLTLKQVLVDRKELTQL
YPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLVTQYPLSNNSVTLNAAKLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGSARFGGASHFLASAQEGTQVKVYVEPNKHFRLPENPETPVIMIGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKIYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLNIEAAEAYFETLRSDKRYQKDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=592, Percent_Identity=30.9121621621622, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI221316709, Length=592, Percent_Identity=30.9121621621622, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI221316705, Length=601, Percent_Identity=30.4492512479201, Blast_Score=246, Evalue=5e-65,
Organism=Homo sapiens, GI127139033, Length=615, Percent_Identity=31.219512195122, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI221316707, Length=520, Percent_Identity=31.1538461538462, Blast_Score=223, Evalue=5e-58,
Organism=Homo sapiens, GI24041029, Length=600, Percent_Identity=30.6666666666667, Blast_Score=209, Evalue=8e-54,
Organism=Homo sapiens, GI10835173, Length=646, Percent_Identity=27.2445820433437, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI40254422, Length=647, Percent_Identity=27.5115919629057, Blast_Score=166, Evalue=8e-41,
Organism=Homo sapiens, GI169790956, Length=408, Percent_Identity=30.8823529411765, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI169790958, Length=408, Percent_Identity=30.8823529411765, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1789123, Length=593, Percent_Identity=51.7706576728499, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17554134, Length=596, Percent_Identity=29.6979865771812, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17566446, Length=573, Percent_Identity=29.8429319371728, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17531441, Length=261, Percent_Identity=27.2030651340996, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=34.4, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321832, Length=650, Percent_Identity=30.3076923076923, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6321143, Length=392, Percent_Identity=32.1428571428571, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325305, Length=632, Percent_Identity=22.9430379746835, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322597, Length=151, Percent_Identity=31.1258278145695, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17137192, Length=613, Percent_Identity=29.3637846655791, Blast_Score=225, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24582192, Length=522, Percent_Identity=29.6934865900383, Blast_Score=192, Evalue=6e-49,
Organism=Drosophila melanogaster, GI24660907, Length=595, Percent_Identity=27.0588235294118, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24660903, Length=595, Percent_Identity=27.0588235294118, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI78706872, Length=526, Percent_Identity=28.5171102661597, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24583543, Length=526, Percent_Identity=28.5171102661597, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI78706876, Length=526, Percent_Identity=28.5171102661597, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24647438, Length=236, Percent_Identity=27.9661016949153, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 65714; Mature: 65714

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPVAQAQTAQT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCEE
VTILYGSQTGNGRGVAKALADKAQAQGYAVNLASMGEYNVRQLKQEAVLLLVVSTHGEGE
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHCCEEEEEEEECCCCCC
APDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQTGKDFDARLAVLGAKSLLPL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHEEEHHHHHCCHH
IECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLVS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEEE
QKITGRGSDRDVRHVEIDLGDSGLTYQAGDALGVWFSNNEALVEEILTALSLSGDEEVVV
EECCCCCCCCCCEEEEEEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEE
EKESLTLKQVLVDRKELTQLYPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLV
EHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
TQYPLSNNSVTLNAAKLLELLRPLTPRLYSIASSQSEVETEVHLTVALVEDERHGSARFG
HHCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCHHCCEEEEEEEEEEECCCCCCCCCC
GASHFLASAQEGTQVKVYVEPNKHFRLPENPETPVIMIGPGTGVAPFRAFMQERVAQGIQ
CHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCC
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKIYVQHRIKEQGQALW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QWLQNGAHLYICGDAERMAKDVHQALIEVAVEVGGLNIEAAEAYFETLRSDKRYQKDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPVAQAQTAQT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCEE
VTILYGSQTGNGRGVAKALADKAQAQGYAVNLASMGEYNVRQLKQEAVLLLVVSTHGEGE
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHCCEEEEEEEECCCCCC
APDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQTGKDFDARLAVLGAKSLLPL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHEEEHHHHHCCHH
IECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLVS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEEE
QKITGRGSDRDVRHVEIDLGDSGLTYQAGDALGVWFSNNEALVEEILTALSLSGDEEVVV
EECCCCCCCCCCEEEEEEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEE
EKESLTLKQVLVDRKELTQLYPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLV
EHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
TQYPLSNNSVTLNAAKLLELLRPLTPRLYSIASSQSEVETEVHLTVALVEDERHGSARFG
HHCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCHHCCEEEEEEEEEEECCCCCCCCCC
GASHFLASAQEGTQVKVYVEPNKHFRLPENPETPVIMIGPGTGVAPFRAFMQERVAQGIQ
CHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCC
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKIYVQHRIKEQGQALW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QWLQNGAHLYICGDAERMAKDVHQALIEVAVEVGGLNIEAAEAYFETLRSDKRYQKDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]