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The map label for this gene is pepB [H]

Identifier: 120597648

GI number: 120597648

Start: 921494

End: 922786

Strand: Direct

Name: pepB [H]

Synonym: Sputw3181_0817

Alternate gene names: 120597648

Gene position: 921494-922786 (Clockwise)

Preceding gene: 120597637

Following gene: 120597649

Centisome position: 19.57

GC content: 50.43

Gene sequence:

>1293_bases
ATGAGTGAACTAAAAGTAACTGAATTGATGAAAGTGCAATTAACCAAAGAAGCACCAGCCGCCTATTGGGGTAAGGCTGA
TGTGACTTATAGTGCCGACGGTGCACAAATTCATTTAGCGGGTGGCGATGAGCTGCGTCAAATTCAAATGGCGGCCCGCA
AACTACGCAGTCAAGGTATTAGCAGTGTGGTATTAGCTGGACAGCTTTGGGATCTCAATAGCCAGTGGGTATTTGCCCAA
GGTTTCGCAACCGCCAAAACCGGCTATCAAATCCACTGGTGTGGTGATGAGTCTGTTCAAGCAGAATTACAACGCAGGTT
CGATGTGGCAACCTTTGCTCGTCAATTGATCAACGATACGCCTGAAAACCTCTCACCCGTAAAACTTGCTCAACAAGCAG
CAAGATGGTTAGCCGATATTGGTGGCGATAAGGTGAGCTATCGGATTATCGAAGGCGAAGCACTGCTCGAAGAACAGTGG
ATTGGTATCCATGCTGTGGGTCGCGGCAGTGAGCGTCCGCCAGCAATGTTAGAATTAGACTTCAATCCATTAGCCGCCGA
TGCACCCGTGTCTGTCGCGCTTGTGGGTAAAGGGATTACCTTTGATTCTGGCGGTTACAGCATTAAAGCCTCCGAAGGCA
TGCTCGGCATGAAATGCGATATGGGCGGCGCCGCCACTGTAACTGCAGCACTCGGTTTAGCAATGAAATCGGGGCTGAAT
AAACGGGTTAAGTTATTCCTTTGCTGTGCTGAAAACTTGATCAGCGGCCGCGCTTATAAGTTAGGCGATATCCTGACTTA
CAAAAATGGCGTGACAGTGGAAGTGGTGAACACCGATGCTGAAGGGCGCTTAGTGTTGGCCGATGGTTTACAAGCGGCTT
CTGCTACTGGAGCGCCTTTTATTATCGATGCGGCGACCCTAACAGGCGCCGCAGTGATGGCGGTAGGTTCAAACTATAAC
GCAATTTTTTCGCCACAGACCGATGTGCTGCAACTGGCTCAGCAAAAAGCGACAAGTGTTGCTGAGCGCGTATGGCCGCT
GCCGCTAGACCCTTGGCACAAAGAAATGTGTCCATCGGCTTACGCTGATACGGCTAATAGTCGTCCGGTGAAAGGTGGCG
GTGCAGGTGGTGCCTCTAATGCCGCTGGTTTCTTATGGCGTTTCGTGTCACCCGAAGCTAAATGGTTACATGTGGATCTC
GCCGCCGCATTTGAAGATTCAGCTTCTGCCCTATGGGGCGCAGGCGCTACGACTCATGGCGTATTGACTATTAGCGAATT
GATTAAAGGCTAA

Upstream 100 bases:

>100_bases
TCGCTTTGAGTCATCGCCTGCTAGCAGTAAACTGCCGCCATAAAGTTTGTTCTGATTGCTGATTGATGTTCTAAAACGAA
TCGAAAAATAAGGATCCCTA

Downstream 100 bases:

>100_bases
GTTAAACCATCAGAAAATACGCTAGCACAGATCCATTGAGCTTCTGTCGCGTTATTTAAAACCGCCTTCGGGCGGTTTTT
TGTTGAGTCACATTTGAGAT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSELKVTELMKVQLTKEAPAAYWGKADVTYSADGAQIHLAGGDELRQIQMAARKLRSQGISSVVLAGQLWDLNSQWVFAQ
GFATAKTGYQIHWCGDESVQAELQRRFDVATFARQLINDTPENLSPVKLAQQAARWLADIGGDKVSYRIIEGEALLEEQW
IGIHAVGRGSERPPAMLELDFNPLAADAPVSVALVGKGITFDSGGYSIKASEGMLGMKCDMGGAATVTAALGLAMKSGLN
KRVKLFLCCAENLISGRAYKLGDILTYKNGVTVEVVNTDAEGRLVLADGLQAASATGAPFIIDAATLTGAAVMAVGSNYN
AIFSPQTDVLQLAQQKATSVAERVWPLPLDPWHKEMCPSAYADTANSRPVKGGGAGGASNAAGFLWRFVSPEAKWLHVDL
AAAFEDSASALWGAGATTHGVLTISELIKG

Sequences:

>Translated_430_residues
MSELKVTELMKVQLTKEAPAAYWGKADVTYSADGAQIHLAGGDELRQIQMAARKLRSQGISSVVLAGQLWDLNSQWVFAQ
GFATAKTGYQIHWCGDESVQAELQRRFDVATFARQLINDTPENLSPVKLAQQAARWLADIGGDKVSYRIIEGEALLEEQW
IGIHAVGRGSERPPAMLELDFNPLAADAPVSVALVGKGITFDSGGYSIKASEGMLGMKCDMGGAATVTAALGLAMKSGLN
KRVKLFLCCAENLISGRAYKLGDILTYKNGVTVEVVNTDAEGRLVLADGLQAASATGAPFIIDAATLTGAAVMAVGSNYN
AIFSPQTDVLQLAQQKATSVAERVWPLPLDPWHKEMCPSAYADTANSRPVKGGGAGGASNAAGFLWRFVSPEAKWLHVDL
AAAFEDSASALWGAGATTHGVLTISELIKG
>Mature_429_residues
SELKVTELMKVQLTKEAPAAYWGKADVTYSADGAQIHLAGGDELRQIQMAARKLRSQGISSVVLAGQLWDLNSQWVFAQG
FATAKTGYQIHWCGDESVQAELQRRFDVATFARQLINDTPENLSPVKLAQQAARWLADIGGDKVSYRIIEGEALLEEQWI
GIHAVGRGSERPPAMLELDFNPLAADAPVSVALVGKGITFDSGGYSIKASEGMLGMKCDMGGAATVTAALGLAMKSGLNK
RVKLFLCCAENLISGRAYKLGDILTYKNGVTVEVVNTDAEGRLVLADGLQAASATGAPFIIDAATLTGAAVMAVGSNYNA
IFSPQTDVLQLAQQKATSVAERVWPLPLDPWHKEMCPSAYADTANSRPVKGGGAGGASNAAGFLWRFVSPEAKWLHVDLA
AAFEDSASALWGAGATTHGVLTISELIKG

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=295, Percent_Identity=37.2881355932203, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI47155554, Length=262, Percent_Identity=38.9312977099237, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI87082123, Length=429, Percent_Identity=49.4172494172494, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1790710, Length=288, Percent_Identity=39.5833333333333, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17556903, Length=299, Percent_Identity=35.4515050167224, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17565172, Length=308, Percent_Identity=26.2987012987013, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI221379063, Length=292, Percent_Identity=36.986301369863, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI221379062, Length=292, Percent_Identity=36.986301369863, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21357381, Length=299, Percent_Identity=36.7892976588629, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355725, Length=295, Percent_Identity=32.2033898305085, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24661038, Length=295, Percent_Identity=32.2033898305085, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24662227, Length=294, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161077148, Length=294, Percent_Identity=30.6122448979592, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20130057, Length=294, Percent_Identity=30.6122448979592, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20129969, Length=292, Percent_Identity=28.7671232876712, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21355645, Length=292, Percent_Identity=27.3972602739726, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24662223, Length=292, Percent_Identity=27.3972602739726, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20129963, Length=294, Percent_Identity=29.5918367346939, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922386, Length=427, Percent_Identity=24.3559718969555, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24646701, Length=207, Percent_Identity=29.4685990338164, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24646703, Length=207, Percent_Identity=29.4685990338164, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21358201, Length=207, Percent_Identity=29.4685990338164, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 45542; Mature: 45411

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELKVTELMKVQLTKEAPAAYWGKADVTYSADGAQIHLAGGDELRQIQMAARKLRSQGI
CCCCCHHHHHEEEECCCCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCC
SSVVLAGQLWDLNSQWVFAQGFATAKTGYQIHWCGDESVQAELQRRFDVATFARQLINDT
CEEEEEEEEECCCCCEEEECCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
PENLSPVKLAQQAARWLADIGGDKVSYRIIEGEALLEEQWIGIHAVGRGSERPPAMLELD
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCEEEEECCCCCCCCEEEEEC
FNPLAADAPVSVALVGKGITFDSGGYSIKASEGMLGMKCDMGGAATVTAALGLAMKSGLN
CCCCCCCCCEEEEEEECCEEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCCC
KRVKLFLCCAENLISGRAYKLGDILTYKNGVTVEVVNTDAEGRLVLADGLQAASATGAPF
CEEEEEEEEHHHHHCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCE
IIDAATLTGAAVMAVGSNYNAIFSPQTDVLQLAQQKATSVAERVWPLPLDPWHKEMCPSA
EEEEHHHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCH
YADTANSRPVKGGGAGGASNAAGFLWRFVSPEAKWLHVDLAAAFEDSASALWGAGATTHG
HHCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCEEEEEEEEEHHCCCCHHHCCCCCCCCH
VLTISELIKG
HEEHHHHHCC
>Mature Secondary Structure 
SELKVTELMKVQLTKEAPAAYWGKADVTYSADGAQIHLAGGDELRQIQMAARKLRSQGI
CCCCHHHHHEEEECCCCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCC
SSVVLAGQLWDLNSQWVFAQGFATAKTGYQIHWCGDESVQAELQRRFDVATFARQLINDT
CEEEEEEEEECCCCCEEEECCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
PENLSPVKLAQQAARWLADIGGDKVSYRIIEGEALLEEQWIGIHAVGRGSERPPAMLELD
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCEEEEECCCCCCCCEEEEEC
FNPLAADAPVSVALVGKGITFDSGGYSIKASEGMLGMKCDMGGAATVTAALGLAMKSGLN
CCCCCCCCCEEEEEEECCEEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCCC
KRVKLFLCCAENLISGRAYKLGDILTYKNGVTVEVVNTDAEGRLVLADGLQAASATGAPF
CEEEEEEEEHHHHHCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCE
IIDAATLTGAAVMAVGSNYNAIFSPQTDVLQLAQQKATSVAERVWPLPLDPWHKEMCPSA
EEEEHHHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCH
YADTANSRPVKGGGAGGASNAAGFLWRFVSPEAKWLHVDLAAAFEDSASALWGAGATTHG
HHCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCEEEEEEEEEHHCCCCHHHCCCCCCCCH
VLTISELIKG
HEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA