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The map label for this gene is macA [H]

Identifier: 120597603

GI number: 120597603

Start: 861069

End: 862319

Strand: Direct

Name: macA [H]

Synonym: Sputw3181_0772

Alternate gene names: 120597603

Gene position: 861069-862319 (Clockwise)

Preceding gene: 120597602

Following gene: 120597604

Centisome position: 18.29

GC content: 42.61

Gene sequence:

>1251_bases
ATGAAAAAATCCTCAAAGCGTAAACTGCTGATTGTGATTAGCGGCCTTATTATCATTGGTGGGGGAGCATTTTCCCTGTG
GTATAAACCAGAAACACCGCCAGCCTATGTTACAGAAGCGGTATATATCGGTGATATTGAAAGTACAGTGCTTGCAAATG
GTACGTTACAGGCATTAAAGCAAGTCAGTGTTGGTGCACAGGTGTCGGGGCAGATTGACAGTTTGTTTGTACAGTCAGGT
GATGAGGTTAAGCAAGGCGATTTAATTGCTCAAATCGACAGCTTGGCACAACAGAATACGATCAAAGAAGCTTTAGCCTC
ACTGAAAATTATCAATGCTCAATATCGTGCAAAGCAAGCACAAATACAGCAAGCCAAACTTGAGTTTGTTCGTCAACAAC
AAATGCTAGCAGATAATGCTAGTTCTCGAGCGTCTTATGAAGCGGCAGAAGCAACTTTGACTGTTTATCAAGCTGAAATA
GAACAATTAGAGGCTCAAAAACAACAGGCTGAAATTAAAGTTGATAGTGCCAAGATTGATTTAGGTTATACCAAAATTAC
CGCGCCTATGGATGGCACCGTTGTATATTCTGCTGTCGAAGTTGGGCAAACCGTTAACGCGAATCAAACGACACCAACCA
TAGTTGAAATGGCACAGCTTGATACTATGACCGTCAAGGCTGAAATATCAGAGGCTGATATTATCAACGTTAAACCTGGC
CAAGCTGTTTACTTTACGATTCTTGGACAACCTAATCGTCCCTATCACGCGACCATTAGGGCAATTGAGCCGGGACCGAC
TTCAATGGAGAGCAGTAGTAGTTCAAGTTCAAGCTCAAGCACGACAGAAGCTATTTACTACTACGGTTTATTTGATGTGA
AAAATCCAGATCGTACTTTACGTATTGGAATGACAACCCAGATTTCAATTGTGCTTGCTAAAGCAGAGAACGCATTACTC
GTGCCTTCACAAATCTTACGGATTAAGCCAAGGGATAAGCAAACTGCACCGCAGCTAGATAAAGACAAAGATACGCAGTC
TAGCGAACCTAAGCGTCATCGTGGGCCTCAGTATCAAGTACCAGTATTAGTGAATAATCAAGTGCAATACGTGGATGTCA
CCGTAGGTATTAACAACAAAATTAATGCCGAAATTACCTCAGGTTTAAATGAAGGCGACTTAGTTGTGCTGGGTATGCCT
TCATCGGAAGAGAGCAGCAAACTGCGCCGCCCACCTAGTGTGAGGTTTTAA

Upstream 100 bases:

>100_bases
TACTGTCTTATATTAACTCTATATACTACCTACATAATTTAATCTTTCTACGTGAGCAATATGCTTGCGGCTTTTTAGTT
TATTGGATGTGAATATACCG

Downstream 100 bases:

>100_bases
TTGTGAGTGCCGCATTACTTGAAATTTCAGGTTGTTATCGCACTTTTCAGGCGGGAGAACAGCAGCTTACCGTACTAAAA
GATGTGAACTTAACGATTGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKKSSKRKLLIVISGLIIIGGGAFSLWYKPETPPAYVTEAVYIGDIESTVLANGTLQALKQVSVGAQVSGQIDSLFVQSG
DEVKQGDLIAQIDSLAQQNTIKEALASLKIINAQYRAKQAQIQQAKLEFVRQQQMLADNASSRASYEAAEATLTVYQAEI
EQLEAQKQQAEIKVDSAKIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAEISEADIINVKPG
QAVYFTILGQPNRPYHATIRAIEPGPTSMESSSSSSSSSSTTEAIYYYGLFDVKNPDRTLRIGMTTQISIVLAKAENALL
VPSQILRIKPRDKQTAPQLDKDKDTQSSEPKRHRGPQYQVPVLVNNQVQYVDVTVGINNKINAEITSGLNEGDLVVLGMP
SSEESSKLRRPPSVRF

Sequences:

>Translated_416_residues
MKKSSKRKLLIVISGLIIIGGGAFSLWYKPETPPAYVTEAVYIGDIESTVLANGTLQALKQVSVGAQVSGQIDSLFVQSG
DEVKQGDLIAQIDSLAQQNTIKEALASLKIINAQYRAKQAQIQQAKLEFVRQQQMLADNASSRASYEAAEATLTVYQAEI
EQLEAQKQQAEIKVDSAKIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAEISEADIINVKPG
QAVYFTILGQPNRPYHATIRAIEPGPTSMESSSSSSSSSSTTEAIYYYGLFDVKNPDRTLRIGMTTQISIVLAKAENALL
VPSQILRIKPRDKQTAPQLDKDKDTQSSEPKRHRGPQYQVPVLVNNQVQYVDVTVGINNKINAEITSGLNEGDLVVLGMP
SSEESSKLRRPPSVRF
>Mature_416_residues
MKKSSKRKLLIVISGLIIIGGGAFSLWYKPETPPAYVTEAVYIGDIESTVLANGTLQALKQVSVGAQVSGQIDSLFVQSG
DEVKQGDLIAQIDSLAQQNTIKEALASLKIINAQYRAKQAQIQQAKLEFVRQQQMLADNASSRASYEAAEATLTVYQAEI
EQLEAQKQQAEIKVDSAKIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAEISEADIINVKPG
QAVYFTILGQPNRPYHATIRAIEPGPTSMESSSSSSSSSSTTEAIYYYGLFDVKNPDRTLRIGMTTQISIVLAKAENALL
VPSQILRIKPRDKQTAPQLDKDKDTQSSEPKRHRGPQYQVPVLVNNQVQYVDVTVGINNKINAEITSGLNEGDLVVLGMP
SSEESSKLRRPPSVRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=399, Percent_Identity=36.3408521303258, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082036, Length=184, Percent_Identity=32.0652173913043, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787013, Length=284, Percent_Identity=23.5915492957746, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789929, Length=295, Percent_Identity=27.7966101694915, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45280; Mature: 45280

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSSKRKLLIVISGLIIIGGGAFSLWYKPETPPAYVTEAVYIGDIESTVLANGTLQALK
CCCCCCCEEEEEEEEEEEECCCEEEEEECCCCCHHHEEEEEEECCCCHHEECCHHHHHHH
QVSVGAQVSGQIDSLFVQSGDEVKQGDLIAQIDSLAQQNTIKEALASLKIINAQYRAKQA
HHHCCCEECCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHH
QIQQAKLEFVRQQQMLADNASSRASYEAAEATLTVYQAEIEQLEAQKQQAEIKVDSAKID
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEHHHHHHHHHHHHHHCEEEEECEEEE
LGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAEISEADIINVKPG
ECEEEEECCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCEEEEECCC
QAVYFTILGQPNRPYHATIRAIEPGPTSMESSSSSSSSSSTTEAIYYYGLFDVKNPDRTL
CEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEE
RIGMTTQISIVLAKAENALLVPSQILRIKPRDKQTAPQLDKDKDTQSSEPKRHRGPQYQV
EEECEEEEEEEEEECCCEEECCHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEE
PVLVNNQVQYVDVTVGINNKINAEITSGLNEGDLVVLGMPSSEESSKLRRPPSVRF
EEEECCCEEEEEEEEECCCCCCCHHHCCCCCCCEEEEECCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MKKSSKRKLLIVISGLIIIGGGAFSLWYKPETPPAYVTEAVYIGDIESTVLANGTLQALK
CCCCCCCEEEEEEEEEEEECCCEEEEEECCCCCHHHEEEEEEECCCCHHEECCHHHHHHH
QVSVGAQVSGQIDSLFVQSGDEVKQGDLIAQIDSLAQQNTIKEALASLKIINAQYRAKQA
HHHCCCEECCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHH
QIQQAKLEFVRQQQMLADNASSRASYEAAEATLTVYQAEIEQLEAQKQQAEIKVDSAKID
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEHHHHHHHHHHHHHHCEEEEECEEEE
LGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAEISEADIINVKPG
ECEEEEECCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCEEEEECCC
QAVYFTILGQPNRPYHATIRAIEPGPTSMESSSSSSSSSSTTEAIYYYGLFDVKNPDRTL
CEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEE
RIGMTTQISIVLAKAENALLVPSQILRIKPRDKQTAPQLDKDKDTQSSEPKRHRGPQYQV
EEECEEEEEEEEEECCCEEECCHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEE
PVLVNNQVQYVDVTVGINNKINAEITSGLNEGDLVVLGMPSSEESSKLRRPPSVRF
EEEECCCEEEEEEEEECCCCCCCHHHCCCCCCCEEEEECCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]