| Definition | Shewanella sp. W3-18-1 chromosome, complete genome. |
|---|---|
| Accession | NC_008750 |
| Length | 4,708,380 |
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The map label for this gene is metE [H]
Identifier: 120597602
GI number: 120597602
Start: 858444
End: 860714
Strand: Direct
Name: metE [H]
Synonym: Sputw3181_0771
Alternate gene names: 120597602
Gene position: 858444-860714 (Clockwise)
Preceding gene: 120597600
Following gene: 120597603
Centisome position: 18.23
GC content: 51.61
Gene sequence:
>2271_bases ATGCAATTAAATAGTCTTGGGTTTCCCCGTATCGGGCGTCGTCGTGAATTGAAATTTGCCTTAGAAAAATACTGGCGTGG TGAGAGCACTCAGGCACAATTGCGTGAAGTGGCGAGTGAACTTCGCCGCAACCATTGGCAGTGGCAAGCTGCTGCTGGCA TTGAGCAAGTTCCCGTAGGTGATTTTGCCTTTTATGATCAAGTGCTGACCTTAAGCGCGACTTTAAATGCCATTCCTGAG CGTCATCGTGGTGAAGGAGCAATCGATCTCGATACTTTATTCCGTGTTGCCCGCGGCAGAGCGCCAACGGGCCAAGATGC GCCAGCCTCTGAAATGACCAAGTATTTCAATACTAACTATCACTATCTAGTACCTGAGCTCAGCCAAAACCAAGTGTTTT CGATTGCCTATGAGCAGTTTTTTGAGGAAGTCAGCGAAGCGCAAGCGCTAGGCTATAAAGCCAAACCTGTGCTACTCGGC CCTGTTAGCTACTTGTTCCTTGCTAAAACTGTGGGCCAAGAGTTTGATAAGTTAAGCTTATTACCCAATTTATTAGCTGC TTATGCTGACATTTTGGCGCGTTTTGCTGCTCAGGGTGTGCAATGGGTACAGCTCGATGAGCCTATTCTCGCATTGGAGT TAGATGGTGAATGGCAAGCGGCGATCACTGAGGCGTATCAAGCCCTCAGCACTGCGAAGGTGAAGATCCTTCTTACCAGT TATTACGGCGGTATCGCACACCATCAAGCGCTGGTATCGGCCTTGCCGGTCGCGGGGTTGCATATCGATTTAGTCACAGC GCCGGATCAATTGGCAGTCTTTGCTAATGCGCTTGGTCAAGATCAAATTTTGTCGGCGGGTGTGGTCAATGGCCGCAACG TGTGGGCGGCAGAGGCGGATTTGATTGCCGAACGCATAGGCGCGGTTGCCCGTGATCTCGGAGATCGCTTGTGGATCGGT ACTTCTTGCTCACTCCTGCACAGCCCTGTGGATTTAGAGGTGGAAGCCACGCTTGAGCCCGCGCTGCGTCAACAACTCGC GTTTGCGAAGCAGAAATTATTCGAGCTGGCCAATGTGCGCCAATTGCTACTGGCACCTGAATCGGTCGCGGCAAAAGAGA TTGTGCAAACCTGTTTGGCACGCCGTGAGGCGAAAGCGCAGGCCGCCGACAGTCAAGTGATTGCCCGCGTTGCCGCGTTG ACATCTGCCGATTATGAGCGCGTCAGTGATTTCAGCGTACGCCAAGCAGTGCAGCAGCAGAAATACCGTTTACCTCTGTT GCCGACCACCACCATTGGTTCCTTCCCACAAACACCGGCAATCCGTGGACTACGCAGTCGCTGGCGCAAGGGCGAGCTGA GTGATGCACAATATACTGAGCAATTACAACAAGTCACCCGCGATACTATCGACCGCCAGCTTAAACTCGGTATCGATGTG CTGGTTCATGGTGAGGCCGAGCGCAACGATATGGTGGAATATTTTGGTGAGCAGCTTGCGGGCGTTGGCTTTACCAAAAA TGGTTGGGTGCAAAGCTATGGTTCACGCTGTGTTAAGCCGCCGTTGATCTATGGTGATGTTAGTCGTCCAAAGGCGATGA CGGTTGATTGGGCCGAGTTTGCCCAAAGTCTCACCGACAAGCCTGTAAAAGGCATGCTGACTGGCCCTGTGACGATTCTG CATTGGTCATTCGCCCGTGAAGATATCGGCCGCGATGTGATTGCGACTCAGCTGGCGCTGGCGATCCGTGATGAAGTGGT GGACTTACAAAATGCCGGTATTGGCATTATACAAATCGATGAGCCCGCCTTCCGTGAAGGTCTGCCATTGAAACAAAGTG AGTGGCAAGCCTATTTAGATTGGGCGGTGAATGCCTTTAAACTCAGCGCTGCTGGGGTGATTGATGAGACTCAAATCCAC ACCCACATGTGTTACAGCGAGTTTAACGATACAATTGCCGCGATTGCTGCCATGGATGCCGATGTGATCACTATCGAGAC TTCGCGTTCACGGATGGAGTTACTCAACGCCTTCGAAGACTTTGAATATCCCAATGAAATTGGCCCCGGTGTGTATGATA TTCATTCGCCAAACACCCCAAGTGTGGAGGCCATGGTGCACTTAATCGAGAAAGCGGCACAAAAAGTGCCCGTACGTCAG CTGTGGGTCAACCCAGATTGTGGCCTAAAAACCCGCACTTGGGATGAAGTCGAACCCGCCTTAAAAAACATGGTCGATGC CACACGGGAACTACGTCGCCGACTAGGTTAA
Upstream 100 bases:
>100_bases GTTATTTATCAAGGCTGAAATGCTGAAAAGTGCCAAGAGAAAACAATCTGGTGGGTGCAAGTGCCGAATATGGCTTATCT CAAATAAAGGAAGATATGGT
Downstream 100 bases:
>100_bases TTGTTCACTTATTGATTATCAATAATATCAATAAGATAGAAGGCGGATGTTTATGCATCCGCTTTAATCCTTGAGTTATC GACTTACCATGTTTCGCCTG
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 756; Mature: 756
Protein sequence:
>756_residues MQLNSLGFPRIGRRRELKFALEKYWRGESTQAQLREVASELRRNHWQWQAAAGIEQVPVGDFAFYDQVLTLSATLNAIPE RHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNYHYLVPELSQNQVFSIAYEQFFEEVSEAQALGYKAKPVLLG PVSYLFLAKTVGQEFDKLSLLPNLLAAYADILARFAAQGVQWVQLDEPILALELDGEWQAAITEAYQALSTAKVKILLTS YYGGIAHHQALVSALPVAGLHIDLVTAPDQLAVFANALGQDQILSAGVVNGRNVWAAEADLIAERIGAVARDLGDRLWIG TSCSLLHSPVDLEVEATLEPALRQQLAFAKQKLFELANVRQLLLAPESVAAKEIVQTCLARREAKAQAADSQVIARVAAL TSADYERVSDFSVRQAVQQQKYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGELSDAQYTEQLQQVTRDTIDRQLKLGIDV LVHGEAERNDMVEYFGEQLAGVGFTKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAEFAQSLTDKPVKGMLTGPVTIL HWSFAREDIGRDVIATQLALAIRDEVVDLQNAGIGIIQIDEPAFREGLPLKQSEWQAYLDWAVNAFKLSAAGVIDETQIH THMCYSEFNDTIAAIAAMDADVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNTPSVEAMVHLIEKAAQKVPVRQ LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG
Sequences:
>Translated_756_residues MQLNSLGFPRIGRRRELKFALEKYWRGESTQAQLREVASELRRNHWQWQAAAGIEQVPVGDFAFYDQVLTLSATLNAIPE RHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNYHYLVPELSQNQVFSIAYEQFFEEVSEAQALGYKAKPVLLG PVSYLFLAKTVGQEFDKLSLLPNLLAAYADILARFAAQGVQWVQLDEPILALELDGEWQAAITEAYQALSTAKVKILLTS YYGGIAHHQALVSALPVAGLHIDLVTAPDQLAVFANALGQDQILSAGVVNGRNVWAAEADLIAERIGAVARDLGDRLWIG TSCSLLHSPVDLEVEATLEPALRQQLAFAKQKLFELANVRQLLLAPESVAAKEIVQTCLARREAKAQAADSQVIARVAAL TSADYERVSDFSVRQAVQQQKYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGELSDAQYTEQLQQVTRDTIDRQLKLGIDV LVHGEAERNDMVEYFGEQLAGVGFTKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAEFAQSLTDKPVKGMLTGPVTIL HWSFAREDIGRDVIATQLALAIRDEVVDLQNAGIGIIQIDEPAFREGLPLKQSEWQAYLDWAVNAFKLSAAGVIDETQIH THMCYSEFNDTIAAIAAMDADVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNTPSVEAMVHLIEKAAQKVPVRQ LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG >Mature_756_residues MQLNSLGFPRIGRRRELKFALEKYWRGESTQAQLREVASELRRNHWQWQAAAGIEQVPVGDFAFYDQVLTLSATLNAIPE RHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNYHYLVPELSQNQVFSIAYEQFFEEVSEAQALGYKAKPVLLG PVSYLFLAKTVGQEFDKLSLLPNLLAAYADILARFAAQGVQWVQLDEPILALELDGEWQAAITEAYQALSTAKVKILLTS YYGGIAHHQALVSALPVAGLHIDLVTAPDQLAVFANALGQDQILSAGVVNGRNVWAAEADLIAERIGAVARDLGDRLWIG TSCSLLHSPVDLEVEATLEPALRQQLAFAKQKLFELANVRQLLLAPESVAAKEIVQTCLARREAKAQAADSQVIARVAAL TSADYERVSDFSVRQAVQQQKYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGELSDAQYTEQLQQVTRDTIDRQLKLGIDV LVHGEAERNDMVEYFGEQLAGVGFTKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAEFAQSLTDKPVKGMLTGPVTIL HWSFAREDIGRDVIATQLALAIRDEVVDLQNAGIGIIQIDEPAFREGLPLKQSEWQAYLDWAVNAFKLSAAGVIDETQIH THMCYSEFNDTIAAIAAMDADVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNTPSVEAMVHLIEKAAQKVPVRQ LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=56.5159574468085, Blast_Score=868, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=762, Percent_Identity=44.750656167979, Blast_Score=644, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 84022; Mature: 84022
Theoretical pI: Translated: 5.03; Mature: 5.03
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 2.0 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQLNSLGFPRIGRRRELKFALEKYWRGESTQAQLREVASELRRNHWQWQAAAGIEQVPVG CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC DFAFYDQVLTLSATLNAIPERHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNY HHHHHHHHHHHHHHHHHCCHHHCCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC HYLVPELSQNQVFSIAYEQFFEEVSEAQALGYKAKPVLLGPVSYLFLAKTVGQEFDKLSL EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHH LPNLLAAYADILARFAAQGVQWVQLDEPILALELDGEWQAAITEAYQALSTAKVKILLTS HHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEH YYGGIAHHQALVSALPVAGLHIDLVTAPDQLAVFANALGQDQILSAGVVNGRNVWAAEAD HHCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCHHHHHHCCCCCCCCEEHHHHH LIAERIGAVARDLGDRLWIGTSCSLLHSPVDLEVEATLEPALRQQLAFAKQKLFELANVR HHHHHHHHHHHHHCCCEEECCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH QLLLAPESVAAKEIVQTCLARREAKAQAADSQVIARVAALTSADYERVSDFSVRQAVQQQ HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGELSDAQYTEQLQQVTRDTIDRQLKLGIDV HCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE LVHGEAERNDMVEYFGEQLAGVGFTKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAEF EEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEECCCCCCCEEEECHHHH AQSLTDKPVKGMLTGPVTILHWSFAREDIGRDVIATQLALAIRDEVVDLQNAGIGIIQID HHHHCCCCCCCEECCCEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC EPAFREGLPLKQSEWQAYLDWAVNAFKLSAAGVIDETQIHTHMCYSEFNDTIAAIAAMDA CHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC DVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNTPSVEAMVHLIEKAAQKVPVRQ CEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCHH LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MQLNSLGFPRIGRRRELKFALEKYWRGESTQAQLREVASELRRNHWQWQAAAGIEQVPVG CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC DFAFYDQVLTLSATLNAIPERHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNY HHHHHHHHHHHHHHHHHCCHHHCCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC HYLVPELSQNQVFSIAYEQFFEEVSEAQALGYKAKPVLLGPVSYLFLAKTVGQEFDKLSL EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHH LPNLLAAYADILARFAAQGVQWVQLDEPILALELDGEWQAAITEAYQALSTAKVKILLTS HHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEH YYGGIAHHQALVSALPVAGLHIDLVTAPDQLAVFANALGQDQILSAGVVNGRNVWAAEAD HHCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCHHHHHHCCCCCCCCEEHHHHH LIAERIGAVARDLGDRLWIGTSCSLLHSPVDLEVEATLEPALRQQLAFAKQKLFELANVR HHHHHHHHHHHHHCCCEEECCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH QLLLAPESVAAKEIVQTCLARREAKAQAADSQVIARVAALTSADYERVSDFSVRQAVQQQ HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGELSDAQYTEQLQQVTRDTIDRQLKLGIDV HCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE LVHGEAERNDMVEYFGEQLAGVGFTKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAEF EEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEECCCCCCCEEEECHHHH AQSLTDKPVKGMLTGPVTILHWSFAREDIGRDVIATQLALAIRDEVVDLQNAGIGIIQID HHHHCCCCCCCEECCCEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC EPAFREGLPLKQSEWQAYLDWAVNAFKLSAAGVIDETQIHTHMCYSEFNDTIAAIAAMDA CHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC DVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNTPSVEAMVHLIEKAAQKVPVRQ CEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCHH LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12368813 [H]