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The map label for this gene is amtB [C]

Identifier: 120597532

GI number: 120597532

Start: 780594

End: 781844

Strand: Direct

Name: amtB [C]

Synonym: Sputw3181_0701

Alternate gene names: 120597532

Gene position: 780594-781844 (Clockwise)

Preceding gene: 120597531

Following gene: 120597533

Centisome position: 16.58

GC content: 44.36

Gene sequence:

>1251_bases
ATGGAAGAATTAACAAAATTAGGCGTTACAGTCACAGAACTACGCTTTGCACTTGATACCTTTTATTTTTTAATCTCAGG
TGCTTTGGTCATGTGGATGGCTGCGGGGTTTGCCATGCTAGAAGCGGGCTTAGTACGCTCAAAAAATACTACTGAAATTT
TAACTAAAAACGTGGTTCTTTATGCAATTGCCTGTGTGATGTACCTCTTAGTCGGCTACAACATTATGTATGTCGATAAT
ACCGAGGGCGGTTGGATACCCTCCATCGGCACTTTAATCGGTTCACAATCTGCTGATGCAAGCCATGCGTTAGAGTCTGA
CTTCTTCTTCCAAGTGGTGTTTGTGGCAACGGCGATGTCAATCGTTTCAGGTGCAGTGGCTGAACGCATGAAGTTATGGG
CGTTTCTTTGTTTCGCCATCGTCATGACTGGGGTGATTTATCCTATTGAAGGGTATTGGACATGGGGCCAAGGATTTATT
GCCAAACTCGGTTTTGTTGATTTTGCAGGTAGTGGCATAGTGCATATGACTGGCGCTGCAGCTGCAATTGCTGGGGTGCT
CTTACTCGGCGCTCGTAAAGGTAAATACGGCCCTAATGGTCAAGTCAATCCCATTCCTGGTTCTAACTTACCCATGGCAA
CCTTAGGTATGTTTATTCTGTGGATGGGGTGGTTTGGGTTTAATGGTGGCTCTCAACTTATGGTGTCTGATGCTGAAAAC
GCAAGTTCAGTGGCAAAAGTGTTTATGAATACCAATACTGCCGCGGCATTTGGCGCTATTTCTGCCCTTATTGTATGTAA
AATGATTTGGGGGAAAGCAGACTTAACAATGATTTTAAACGGTATTCTAGCGGGTTTAGTCGCAATCACTGCCGATCCTT
TATCTCCTTCATTTTTAATGGCGGGCGTCATTGGCTTGGTTGCTGGTGGTGTAGTGGTGTTCTCCATTGTCGGATTAGAT
CGCGTTAAGATTGATGATCCTGTCGGGGCTATTTCAGTTCATGGTGTGGCAGGTTTTCTTGGTCTGATGTGCGTACCTTT
ATCGAATGCTGATGCAACCATTACGGCACAGCTAACTGGTGCTGCGATTATCTTTGCTTGGGTATTTTGTTCCTCATTTG
TCGTATGGTCTATCATCAAAATGACTATGGGTATTCGAGTTTCTGAGGAGGAAGAATATAACGGTATGGATGCTTCCGAT
TGCGGTATCGACGCTTATCCTGAGTTTGTGACAGTAAAAAATGCCAGCTAA

Upstream 100 bases:

>100_bases
GGAAAAATTGGCGACGGTAAAATTTTCGTCGTTGACTTAGAACATGCTATCCGTATCCGTACGGGTGAACTCGACTCTGA
AGCGCTATAAGGGGCTGATA

Downstream 100 bases:

>100_bases
AAACTCACTGATATGACTTGATGCCATAACAGGATCAGGGGATAGTAAAGCAGAGCTTAGTGCTCTGCTTTTTTATGGGT
AATCCAAGCCAAGTGTGAGG

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MEELTKLGVTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVLYAIACVMYLLVGYNIMYVDN
TEGGWIPSIGTLIGSQSADASHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLCFAIVMTGVIYPIEGYWTWGQGFI
AKLGFVDFAGSGIVHMTGAAAAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAEN
ASSVAKVFMNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSFLMAGVIGLVAGGVVVFSIVGLD
RVKIDDPVGAISVHGVAGFLGLMCVPLSNADATITAQLTGAAIIFAWVFCSSFVVWSIIKMTMGIRVSEEEEYNGMDASD
CGIDAYPEFVTVKNAS

Sequences:

>Translated_416_residues
MEELTKLGVTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVLYAIACVMYLLVGYNIMYVDN
TEGGWIPSIGTLIGSQSADASHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLCFAIVMTGVIYPIEGYWTWGQGFI
AKLGFVDFAGSGIVHMTGAAAAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAEN
ASSVAKVFMNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSFLMAGVIGLVAGGVVVFSIVGLD
RVKIDDPVGAISVHGVAGFLGLMCVPLSNADATITAQLTGAAIIFAWVFCSSFVVWSIIKMTMGIRVSEEEEYNGMDASD
CGIDAYPEFVTVKNAS
>Mature_416_residues
MEELTKLGVTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVLYAIACVMYLLVGYNIMYVDN
TEGGWIPSIGTLIGSQSADASHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLCFAIVMTGVIYPIEGYWTWGQGFI
AKLGFVDFAGSGIVHMTGAAAAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAEN
ASSVAKVFMNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSFLMAGVIGLVAGGVVVFSIVGLD
RVKIDDPVGAISVHGVAGFLGLMCVPLSNADATITAQLTGAAIIFAWVFCSSFVVWSIIKMTMGIRVSEEEEYNGMDASD
CGIDAYPEFVTVKNAS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=410, Percent_Identity=30.2439024390244, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=34.8993288590604, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17550182, Length=430, Percent_Identity=34.6511627906977, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=27.5534441805226, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=334, Percent_Identity=27.8443113772455, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=29.0094339622642, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=28.7769784172662, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321559, Length=443, Percent_Identity=27.765237020316, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161078314, Length=407, Percent_Identity=33.9066339066339, Blast_Score=174, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43934; Mature: 43934

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELTKLGVTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YAIACVMYLLVGYNIMYVDNTEGGWIPSIGTLIGSQSADASHALESDFFFQVVFVATAMS
HHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLCFAIVMTGVIYPIEGYWTWGQGFIAKLGFVDFAGSGIVHMTGAA
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCHHHHHHHCCHHCCCCCEEEECCHH
AAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAEN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ASSVAKVFMNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSFLM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGVIGLVAGGVVVFSIVGLDRVKIDDPVGAISVHGVAGFLGLMCVPLSNADATITAQLTG
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHHEECCCCCCEEEEEHHH
AAIIFAWVFCSSFVVWSIIKMTMGIRVSEEEEYNGMDASDCGIDAYPEFVTVKNAS
HHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MEELTKLGVTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YAIACVMYLLVGYNIMYVDNTEGGWIPSIGTLIGSQSADASHALESDFFFQVVFVATAMS
HHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLCFAIVMTGVIYPIEGYWTWGQGFIAKLGFVDFAGSGIVHMTGAA
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCHHHHHHHCCHHCCCCCEEEECCHH
AAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAEN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ASSVAKVFMNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSFLM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGVIGLVAGGVVVFSIVGLDRVKIDDPVGAISVHGVAGFLGLMCVPLSNADATITAQLTG
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHHEECCCCCCEEEEEHHH
AAIIFAWVFCSSFVVWSIIKMTMGIRVSEEEEYNGMDASDCGIDAYPEFVTVKNAS
HHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]