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The map label for this gene is miaA

Identifier: 120597491

GI number: 120597491

Start: 728154

End: 729068

Strand: Direct

Name: miaA

Synonym: Sputw3181_0660

Alternate gene names: 120597491

Gene position: 728154-729068 (Clockwise)

Preceding gene: 120597490

Following gene: 120597492

Centisome position: 15.47

GC content: 44.37

Gene sequence:

>915_bases
TTGCTGCCTAAAGTGTTATTTCTTATGGGGCCTACTGCCTCTGGAAAAACGGCTTTAGCACTAGAATTAGCCGAAAACCA
TAACTGCGAGATTATCTCAGTTGATTCTGCACTCATTTATCGCGGTATGGATATTGGTAGTGCAAAACCTTCTATAGAGG
AGTTAGCGCGCGGGCCACATAGATTGATTGATATTCGCGATCCTAGCGAGAGCTATTCTGCCGCCGATTTTAGAGCAGAT
GCTTTAGCTGAAATTGAGCAAATTATTCGCATGGGGAAAACACCATTACTCGTTGGTGGAACCATGATGTATTTTAAGGC
TCTATTAGAAGGGTTATCACCGCTTCCGAGTGCCGATGACGCCATTCGTGCCGACATTCAGGCAGAGGCTGATGCGAAAG
GATGGGAAACCTTGCATGACCAATTACGGGATATTGATCCCGTGTCGGCGGAAAGAATTCATCCTAATGATCCACAAAGG
CTTTCAAGAGCGCTGGAAGTCTATAGAATAAGCGGTAAGAGTTTAACTGAGCTTACACAGACTAAGTCTGCACCTTTACC
TTACGATGTGGTGCAATTTGCCATTGCACCGCGAGAGCGTAAAGTATTACATGACTTAATTGCGCAGCGTTTTCGCATAA
TGTTACAGCAAGGTTTTATTGGCGAAGTGACTCAACTTAAGGCAAGGGGAGACTTACATTTAGATTTGCCATCAATGCGT
TGTGTTGGATATCGGCAATGTTGGCAACATCTCGATGGAGAATTCGACTATGATACTATGGTCGAAAAAGCAGTTGCAGC
TACTAGGCAATTAGCGAAGCGTCAATTAACATGGTTACGTAGTTGGCCAGAGTTAAATTGGCTTGAAAGTGGTGCTGAGG
GGAATTTAGTTACACTTATGCGTCATTGCCGCTAG

Upstream 100 bases:

>100_bases
TTTTTCCTTGCTAACGGATGAAACAAGACAGCATATTGCCAATCAAGCGATATCTTTACCTTGGCAATCATGGCTAGAAG
AGTAATAAGTGAATACAGAA

Downstream 100 bases:

>100_bases
CTTATTAGCCCTTGCTGTATAATACTAAAACTAAACAAAAAACTTCACTGATATAGATTTATTTTTATAAATTAAAAAGG
AAATAATAAAATGGCTAAGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLPKVLFLMGPTASGKTALALELAENHNCEIISVDSALIYRGMDIGSAKPSIEELARGPHRLIDIRDPSESYSAADFRAD
ALAEIEQIIRMGKTPLLVGGTMMYFKALLEGLSPLPSADDAIRADIQAEADAKGWETLHDQLRDIDPVSAERIHPNDPQR
LSRALEVYRISGKSLTELTQTKSAPLPYDVVQFAIAPRERKVLHDLIAQRFRIMLQQGFIGEVTQLKARGDLHLDLPSMR
CVGYRQCWQHLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRHCR

Sequences:

>Translated_304_residues
MLPKVLFLMGPTASGKTALALELAENHNCEIISVDSALIYRGMDIGSAKPSIEELARGPHRLIDIRDPSESYSAADFRAD
ALAEIEQIIRMGKTPLLVGGTMMYFKALLEGLSPLPSADDAIRADIQAEADAKGWETLHDQLRDIDPVSAERIHPNDPQR
LSRALEVYRISGKSLTELTQTKSAPLPYDVVQFAIAPRERKVLHDLIAQRFRIMLQQGFIGEVTQLKARGDLHLDLPSMR
CVGYRQCWQHLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRHCR
>Mature_304_residues
MLPKVLFLMGPTASGKTALALELAENHNCEIISVDSALIYRGMDIGSAKPSIEELARGPHRLIDIRDPSESYSAADFRAD
ALAEIEQIIRMGKTPLLVGGTMMYFKALLEGLSPLPSADDAIRADIQAEADAKGWETLHDQLRDIDPVSAERIHPNDPQR
LSRALEVYRISGKSLTELTQTKSAPLPYDVVQFAIAPRERKVLHDLIAQRFRIMLQQGFIGEVTQLKARGDLHLDLPSMR
CVGYRQCWQHLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRHCR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=182, Percent_Identity=35.1648351648352, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=68.1660899653979, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=28.8321167883212, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=28.4745762711864, Blast_Score=128, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24649830, Length=246, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 34128; Mature: 34128

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKVLFLMGPTASGKTALALELAENHNCEIISVDSALIYRGMDIGSAKPSIEELARGPH
CCCCEEEEECCCCCCCEEEEEEECCCCCCEEEEECHHHHHCCCCCCCCCCCHHHHHCCCC
RLIDIRDPSESYSAADFRADALAEIEQIIRMGKTPLLVGGTMMYFKALLEGLSPLPSADD
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCCH
AIRADIQAEADAKGWETLHDQLRDIDPVSAERIHPNDPQRLSRALEVYRISGKSLTELTQ
HHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
TKSAPLPYDVVQFAIAPRERKVLHDLIAQRFRIMLQQGFIGEVTQLKARGDLHLDLPSMR
HCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCHH
CVGYRQCWQHLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHH
RHCR
HHCC
>Mature Secondary Structure
MLPKVLFLMGPTASGKTALALELAENHNCEIISVDSALIYRGMDIGSAKPSIEELARGPH
CCCCEEEEECCCCCCCEEEEEEECCCCCCEEEEECHHHHHCCCCCCCCCCCHHHHHCCCC
RLIDIRDPSESYSAADFRADALAEIEQIIRMGKTPLLVGGTMMYFKALLEGLSPLPSADD
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCCH
AIRADIQAEADAKGWETLHDQLRDIDPVSAERIHPNDPQRLSRALEVYRISGKSLTELTQ
HHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
TKSAPLPYDVVQFAIAPRERKVLHDLIAQRFRIMLQQGFIGEVTQLKARGDLHLDLPSMR
HCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCHH
CVGYRQCWQHLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHH
RHCR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA