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The map label for this gene is amiB [H]

Identifier: 120597489

GI number: 120597489

Start: 724781

End: 726211

Strand: Direct

Name: amiB [H]

Synonym: Sputw3181_0658

Alternate gene names: 120597489

Gene position: 724781-726211 (Clockwise)

Preceding gene: 120597488

Following gene: 120597490

Centisome position: 15.39

GC content: 44.51

Gene sequence:

>1431_bases
ATGACTAAAATTAATCATTATTTTCATCTTATTACTCTTTTTTTATGCACTTTTTTTACTGTATCTGCCCATGGGGCAAA
TCGATTAGAAGGTGTGCGTATTTGGGCTGCCCCTGAATCGACCCGTGTAGTGTTTGATCTCAGTGAAGCGCCGAACTATA
CCTATTTTTCAATAGAAGGGCCTAACCGTTTAGTGGTCGATCTTAAAAAGACTTCAACTAAACTCACCCTTAAAAATATC
GACAATAACAGCAAGCTCGTTAAAGGGGTGCGCATTAGTCAATCTCCAACGAAGGGTGATTTGCGTTTGGTGATTGACCT
TGTAAAGCCTCTTAATGCTAACTTGTTTTCACTTCCCGTTACGGCTCCCTATGGTAATCGTCTTGTAGTTGACCTTGAAG
ATAAAACGGTCACACTGGCACCACCTGCCGCCAGCTCAACCCCAGTTAAAAAGTCCGTTACTCAAAGTATGCAATCCTCC
CGCGATATTGTAATTGCTATCGATGCTGGACATGGTGGTGACGACCCCGGCTCAATTGGTCCATCGGGCCTTTATGAGAA
AAAAGTGGTATTCGAAATTTCTCGGCGCCTTGCGAGTAAAATTAACGACACCCCTGGAATGCGTGCCGTTATGATCCGCA
GTGGCGACTACTTTGTTAATTTAAATAAGCGCTCTGAACTTGCCCGCAACAGTAAAGCTGATTTGCTGATTTCTATCCAT
GCGGATGCGTTTACTTCGCCCAATCCTCGCGGTGCGTCTGTGTGGGTATTGTCTATGCGTCGTGCAAACAGTGAAATTGG
TCGCTGGTTGGAGCAAAAAGAAAAGCATTCTGAACTACTTGGCGGTGCGGGGGAAATTATCCAAAATACCGATAATGAAC
AATATCTTGCTATGACATTACTCGATATGTCGATGAACAGTTCTATGGCTATTGGTCATGCGGTTGCGGGGGATATTCTC
AAGGATTTAGGCGCTGTGACGACGTTGCATAAAAGTCGGCCCGAATCAGCCAGTTTTGCAGTGCTAAAGTCACCGGATAT
CCCCTCAATTTTGGTTGAGACTGGCTTTATTTCTAATCCTAAGGAAGAGCGACTCTTATCCAGTCACCAACACCAAGAAA
GTATTGCAACAGCTATTTATAAAGGGGTTAGCCGATATTTCCATAGCAATCCGCCGGCAGATACTCTGATAGCCAAACAA
AATGGGGCAAGGCCGAGTCCTATATCTAGTTCTAAATCAGCATCTACAGGAATAAGTTCAAGGCCAAGTGTTAGATCTTC
TGGTACGGTCAAACATAAAGTCAGCCGCGGTGAATCGCTTTCAGCCATTGCGCAGCGTTATCAAGTCCCAATGTCGAGTA
TTAAACGTGCGAATGGGATGAAAACCGATGTAGTGCAACTAGGGCAAACGCTCGTTATTCCTGAGAGTTAG

Upstream 100 bases:

>100_bases
ATTTGCATTTGAGTTATGTAAATTCGGGCCGTGAAATCCATATTCAAGCCCTGTCTAAATCAGGTGAAAAGTTATTAGCT
GCGATAAAATAAAAACAACA

Downstream 100 bases:

>100_bases
AATGGAAAAATCTATGGGTATTCAGATCTTACCGCCGCAATTAGCCAACCAGATCGCCGCGGGCGAAGTGGTTGAAAGGC
CCGCCTCAGTGGTTAAAGAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTKINHYFHLITLFLCTFFTVSAHGANRLEGVRIWAAPESTRVVFDLSEAPNYTYFSIEGPNRLVVDLKKTSTKLTLKNI
DNNSKLVKGVRISQSPTKGDLRLVIDLVKPLNANLFSLPVTAPYGNRLVVDLEDKTVTLAPPAASSTPVKKSVTQSMQSS
RDIVIAIDAGHGGDDPGSIGPSGLYEKKVVFEISRRLASKINDTPGMRAVMIRSGDYFVNLNKRSELARNSKADLLISIH
ADAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHAVAGDIL
KDLGAVTTLHKSRPESASFAVLKSPDIPSILVETGFISNPKEERLLSSHQHQESIATAIYKGVSRYFHSNPPADTLIAKQ
NGARPSPISSSKSASTGISSRPSVRSSGTVKHKVSRGESLSAIAQRYQVPMSSIKRANGMKTDVVQLGQTLVIPES

Sequences:

>Translated_476_residues
MTKINHYFHLITLFLCTFFTVSAHGANRLEGVRIWAAPESTRVVFDLSEAPNYTYFSIEGPNRLVVDLKKTSTKLTLKNI
DNNSKLVKGVRISQSPTKGDLRLVIDLVKPLNANLFSLPVTAPYGNRLVVDLEDKTVTLAPPAASSTPVKKSVTQSMQSS
RDIVIAIDAGHGGDDPGSIGPSGLYEKKVVFEISRRLASKINDTPGMRAVMIRSGDYFVNLNKRSELARNSKADLLISIH
ADAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHAVAGDIL
KDLGAVTTLHKSRPESASFAVLKSPDIPSILVETGFISNPKEERLLSSHQHQESIATAIYKGVSRYFHSNPPADTLIAKQ
NGARPSPISSSKSASTGISSRPSVRSSGTVKHKVSRGESLSAIAQRYQVPMSSIKRANGMKTDVVQLGQTLVIPES
>Mature_475_residues
TKINHYFHLITLFLCTFFTVSAHGANRLEGVRIWAAPESTRVVFDLSEAPNYTYFSIEGPNRLVVDLKKTSTKLTLKNID
NNSKLVKGVRISQSPTKGDLRLVIDLVKPLNANLFSLPVTAPYGNRLVVDLEDKTVTLAPPAASSTPVKKSVTQSMQSSR
DIVIAIDAGHGGDDPGSIGPSGLYEKKVVFEISRRLASKINDTPGMRAVMIRSGDYFVNLNKRSELARNSKADLLISIHA
DAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHAVAGDILK
DLGAVTTLHKSRPESASFAVLKSPDIPSILVETGFISNPKEERLLSSHQHQESIATAIYKGVSRYFHSNPPADTLIAKQN
GARPSPISSSKSASTGISSRPSVRSSGTVKHKVSRGESLSAIAQRYQVPMSSIKRANGMKTDVVQLGQTLVIPES

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=436, Percent_Identity=37.6146788990826, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI87082163, Length=394, Percent_Identity=35.2791878172589, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=42.2222222222222, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 51702; Mature: 51570

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKINHYFHLITLFLCTFFTVSAHGANRLEGVRIWAAPESTRVVFDLSEAPNYTYFSIEG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEECC
PNRLVVDLKKTSTKLTLKNIDNNSKLVKGVRISQSPTKGDLRLVIDLVKPLNANLFSLPV
CCEEEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHEEHHCCCCCCEEEEEE
TAPYGNRLVVDLEDKTVTLAPPAASSTPVKKSVTQSMQSSRDIVIAIDAGHGGDDPGSIG
ECCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
PSGLYEKKVVFEISRRLASKINDTPGMRAVMIRSGDYFVNLNKRSELARNSKADLLISIH
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCHHHHHCCCCCCEEEEEE
ADAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTL
CCCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEE
LDMSMNSSMAIGHAVAGDILKDLGAVTTLHKSRPESASFAVLKSPDIPSILVETGFISNP
EHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCC
KEERLLSSHQHQESIATAIYKGVSRYFHSNPPADTLIAKQNGARPSPISSSKSASTGISS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RPSVRSSGTVKHKVSRGESLSAIAQRYQVPMSSIKRANGMKTDVVQLGQTLVIPES
CCCCCCCCCEEEHHCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCEEEECCC
>Mature Secondary Structure 
TKINHYFHLITLFLCTFFTVSAHGANRLEGVRIWAAPESTRVVFDLSEAPNYTYFSIEG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEECC
PNRLVVDLKKTSTKLTLKNIDNNSKLVKGVRISQSPTKGDLRLVIDLVKPLNANLFSLPV
CCEEEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHEEHHCCCCCCEEEEEE
TAPYGNRLVVDLEDKTVTLAPPAASSTPVKKSVTQSMQSSRDIVIAIDAGHGGDDPGSIG
ECCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
PSGLYEKKVVFEISRRLASKINDTPGMRAVMIRSGDYFVNLNKRSELARNSKADLLISIH
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCHHHHHCCCCCCEEEEEE
ADAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTL
CCCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEE
LDMSMNSSMAIGHAVAGDILKDLGAVTTLHKSRPESASFAVLKSPDIPSILVETGFISNP
EHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCC
KEERLLSSHQHQESIATAIYKGVSRYFHSNPPADTLIAKQNGARPSPISSSKSASTGISS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RPSVRSSGTVKHKVSRGESLSAIAQRYQVPMSSIKRANGMKTDVVQLGQTLVIPES
CCCCCCCCCEEEHHCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]