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The map label for this gene is rimK1 [H]

Identifier: 120597445

GI number: 120597445

Start: 673355

End: 674260

Strand: Direct

Name: rimK1 [H]

Synonym: Sputw3181_0614

Alternate gene names: 120597445

Gene position: 673355-674260 (Clockwise)

Preceding gene: 120597444

Following gene: 120597450

Centisome position: 14.3

GC content: 43.49

Gene sequence:

>906_bases
ATGAAAATAGGTATCTTATCCCAGTTTCCTCAACTGTATTCAACGCAACGTTTAGTCGCCGCCTGTGAGAGTCGTGGCCA
TGAAGCGGTTGTGATCAATACTCTGAACTGTTATATGAATATCAACTCGATCAAACCCAGTATTCATTATGAGGGACAGG
AGTTGGTTGGATTCGATGCAATCATTCCTCGAATCCATGCAAGTGTGACTTTTTATGGTTGTGCAGTGGTACGTCAGTTT
GAAATGATGGGTGTATTTGTGGCTAATGACTCGATTTCGATAGCCCGATCCCGTGATAAGTTAAGGGCATTGCAGTTGCT
TTCTCGTAAAGGGATAGGTATGCCAATTACGGGGTTTGCGAATAAACCTAATGATATTCCTGATTTAATCAATATGGTGG
GAGGTGCTCCCTTAGTGATTAAACTGCTTGAGGGTACTCAAGGTATTGGTGTTGTGTTAGCAGAGACGAAAACCGCGGCA
GAGAGCGTGATCGAAGCTTTTTTAGGATTGAAAGCCAATATCTTAGTGCAGGAATATATTAAAGAGTCTAACGGAAGCGA
TATTCGCTGTTTTGTTGTAGGTGATAAAGTTGTCGCTTCGATGAAACGCCAAGGACCAGAAGGAGATTTTCGCTCTAATC
TTCATTTGGGCGGTTGTGGTGAAACGGTAAAAATCACCTCCGTCGAGCGTAAGATGGCGATTGCAGCCGTAAAGGCCATG
GGGCTTGTTGTCGCTGGTGTGGATATTTTACGCTCTAACCGTGGCCCTTTAATTCTTGAAGTGAACTCGGCACCTGGCAT
TGAAGGTATTGAACAAACCACAGGGATTTCAGTCACTGAACCGATAGTTGAATATATCGAGAAAATGGTTGCAGCGCGTA
AAACAAATAGGCCAATCATTGCTTAA

Upstream 100 bases:

>100_bases
TTACACTGAGCAATAGACAAATATCAGCCCCCCTTTTCTGATGCCGAATTTTGTTGTGTCAGAACTCTTTATTCACTGTG
CTTAAGTGGGTCATTCCGTA

Downstream 100 bases:

>100_bases
TGGATCATTGCGATAGCACAACGCCACCGCTTAAGGTGGCGTTTTGATAACTTGTACTCGCTCGGGGTAGATAGTAAAGC
TTAGAGGTCAAAGCTGTAGG

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIGILSQFPQLYSTQRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYEGQELVGFDAIIPRIHASVTFYGCAVVRQF
EMMGVFVANDSISIARSRDKLRALQLLSRKGIGMPITGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANILVQEYIKESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGETVKITSVERKMAIAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPIIA

Sequences:

>Translated_301_residues
MKIGILSQFPQLYSTQRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYEGQELVGFDAIIPRIHASVTFYGCAVVRQF
EMMGVFVANDSISIARSRDKLRALQLLSRKGIGMPITGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANILVQEYIKESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGETVKITSVERKMAIAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPIIA
>Mature_301_residues
MKIGILSQFPQLYSTQRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYEGQELVGFDAIIPRIHASVTFYGCAVVRQF
EMMGVFVANDSISIARSRDKLRALQLLSRKGIGMPITGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANILVQEYIKESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGETVKITSVERKMAIAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPIIA

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=226, Percent_Identity=29.646017699115, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI134254462, Length=225, Percent_Identity=28, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1787076, Length=288, Percent_Identity=60.7638888888889, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32423; Mature: 32423

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILSQFPQLYSTQRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYEGQELVGFDA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCEEECCCHHCCHHH
IIPRIHASVTFYGCAVVRQFEMMGVFVANDSISIARSRDKLRALQLLSRKGIGMPITGFA
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
NKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAAESVIEAFLGLKANILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
KESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGETVKITSVERKMAIAAVKAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHH
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPII
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
A
C
>Mature Secondary Structure
MKIGILSQFPQLYSTQRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYEGQELVGFDA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCEEECCCHHCCHHH
IIPRIHASVTFYGCAVVRQFEMMGVFVANDSISIARSRDKLRALQLLSRKGIGMPITGFA
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
NKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAAESVIEAFLGLKANILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
KESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGETVKITSVERKMAIAAVKAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHH
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPII
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA