Click here to switch to the map view.

The map label for this gene is aceE

Identifier: 120597357

GI number: 120597357

Start: 564822

End: 567488

Strand: Direct

Name: aceE

Synonym: Sputw3181_0526

Alternate gene names: 120597357

Gene position: 564822-567488 (Clockwise)

Preceding gene: 120597356

Following gene: 120597358

Centisome position: 12.0

GC content: 47.02

Gene sequence:

>2667_bases
ATGTCTGAAGATATGCTACAAGACGTAGATCCGTTAGAGACTCAGGAATGGGTTGATGCCCTGCAAGCCGTATTGGAACA
AGAAGGCCCAGAACGCGCACATTTTTTATTAGAAAAGTTGATCGATAAAGCCCGTCGTAACGGCACTCACTTGCCTTACA
ACGCGACAACCGCTTATCTAAACACTATCCCTGCGGGTCAAGAGCCGCATATGCCGGGCAACCAAGAGATGGAACGTCGC
ATTCGCGCCATCATCCGTTGGAACGCCTTAGCTATGGTGCTGCGTGGTTCTAAGAAAGATTTAGAATTAGGCGGTCATAT
CTCCAGTTTCGCCTCAAGCGCGACTATCTATGACGTGTGTTTTAACCACTTCTTCCGCGCACCGAATGAAAAAGACGGCG
GCGATCTCGTGTATTTCCAAGGCCATATTGCCCCTGGTATTTATGCGCGTTCATTCTTAGAAGGTCGTTTAACGGAAGAC
CAACTGGCTAACTTCCGTCAAGAAGTGGATGGCAAGGGCTTGTCTTCATACCCACATCCAAAACTGATGCCGGATTACTG
GCAATTCCCAACGGTTTCTATGGGCCTAGGTCCTATCCAAGCGATTTATCAGGCGCGTTTCCTGAAATATTTGACTGACC
GTGGCCTGAAAGATTGTTCAGCACAAACCGTTTACTGTTTCTTAGGTGACGGCGAGTGTGATGAGCCAGAAGCCTTAGGT
GCGATTGGTTTAGCGGCCCGTGAAGAATTAGATAACTTAGTCTTTATCGTTAACTGTAACTTACAGCGTCTTGATGGTCC
TGTACGTGGTAACGGTAAGATCATCCAAGAACTTGAAGGCGAATTCCGTGGCGCAGGCTGGGAAGTGGTTAAAGTTATTT
GGGGTCGTTACTGGGATCCATTATTGGCCCGTGACACTAGCGGTAAGTTATTACAGTTAATGGAAGAAACCGTAGACGGT
GAATACCAAAACTGTAAAGCCAAAGGTGGCGCTTATACTCGCGAGCACTTCTTCGGTAAGTACCCAGAGACAGCCGAAAT
GGTAGCGAACATGTCAGACGATGACATTTGGCGCTTAAACCGTGGTGGTCATGATCCAGTGAAAGTGTACGCAGCCTTAG
ATCACGCGCAAAAAACCAAAGGTCGCCCAACGGTGATTTTAGCTAAGACTGTAAAAGGTTACGGCTTAGGTGATGCGGGT
GAAGGTAAAAACATCGCTCACAACGTGAAGAAAATGGGTATCGAGTCTATCCGTTACTTCCGCGATCGTTTCAATATCCC
CATTCCAGATGACCAATTAGAAGACTTACCTTTCTATCATCCAGGTCCTGATTCGGAAGAGGTGAAGTACATGATGAGCC
GTCGTGAAGCGCTGCATGGTAGCGTGCCACAACGTCGTCAAAAGTTCACCGAAGAGCTTGAAATCCCATCACTGAAGATT
TTCGATTCGATTTTACAGGGCTCAAATGGCCGTGAAATTTCATCGACTATGGCATTCGTTCGTGTACTGACAGCGCTGCT
GAAAGACAAGAAAATTGGCAAAAACATTGTGCCAATCATTCCTGACGAAGCGCGTACCTTCGGTATGGAAGGTTTGTTCC
GTCAAGTGGGCATTTATGCTCACGAAGGCCAAAAATATATTCCGCAGGATTCTGACCAAGTTGCTTACTACCGCGAAGAT
AAAACTGGTCAAGTTCTGCAAGAAGGGATTAACGAACTCGGCGCTATGTCTTCATGGGTATCGGCAGCGACTAGCTACTC
AGTCAACAACGTGCCTATGATCCCGTTCTATATCTACTACTCAATGTTCGGTTTCCAACGTATTGGCGACATGGCATGGG
CTGCGGGTGATATGCGTGCTCGTGGTTTCTTAGTGGGCGGTACTTCAGGCCGTACAACCCTGAACGGTGAAGGTTTACAG
CATCAAGATGGTCACAGCCATGTATTGGCAAACACTGTCCCTAACTGTATTTCTTACGACCCAACTTATGGTTATGAAAT
TGCCGTTGTGGTGCAAGATGGTATTCGCCGTATGTACGGTGAAAATCAAGAAGATATCTTCTATTACTTGACCACAATGA
ACGAAAACTATGTTCAGCCTGAAATGCCTGAAGGTGCCGAAGAAGGTATCGTTAAAGGGATTTACAAGCTGGAAACTGTT
TCAGGATCAGGCAAAGGTAAAGTTCAGTTAATGAGCTGTGGCACTATCCTTGAGCAAACTCGTAAAGCGGCGCAAGCATT
AGCGAAAGACTTTGGTATTACTGCCGACGTATTTAGCGTGACCAGCTTCAACGAGTTGACCCGCGATGGCCAAGCTGTTG
AGCGCTGGAACATGCTGCACCCAACGCAAACGCCAAAACAAGCCTATATCAGCCAAGTGATTTCAAGTGATGCACCTGCG
ATTGCGGCGACTGACTATATGAAGATTTATGGCGAGCAGTTACGTGCTTACATGCCAACCGATTACAAAGTGTTAGGTAC
TGACGGTTTTGGTCGCTCAGACAGCCGCGAGAATCTGCGTCACCACTTTGAAGTGGACGCTAAGTTCATCGTGATTGCAG
CGCTGAAATCACTGGTTGATCGTAAAGAGCTACCTGTCGATGTGTTAGCAAACGCCATTAAAGAATATGGTATCGACGCT
GACAAGATCAATCCACAGTACGCGTAA

Upstream 100 bases:

>100_bases
TTAAAGAGTGGCGCTTAATTTCCTGAGATTTAGCACTGAGCTAAATCGGAATATGCCGCAAACACCATTCATTATCATGT
ATATAGAGAAGGACAGTGTC

Downstream 100 bases:

>100_bases
GAGGCAATGAAAATGGCTGAATTAAAAGAAGTTTTTGTTCCTGATATCGGCGGCGACGAAGTGCAAGTGATCGAAATTTG
TGCCGCTGTGGGTGATACCC

Product: pyruvate dehydrogenase subunit E1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 888; Mature: 887

Protein sequence:

>888_residues
MSEDMLQDVDPLETQEWVDALQAVLEQEGPERAHFLLEKLIDKARRNGTHLPYNATTAYLNTIPAGQEPHMPGNQEMERR
IRAIIRWNALAMVLRGSKKDLELGGHISSFASSATIYDVCFNHFFRAPNEKDGGDLVYFQGHIAPGIYARSFLEGRLTED
QLANFRQEVDGKGLSSYPHPKLMPDYWQFPTVSMGLGPIQAIYQARFLKYLTDRGLKDCSAQTVYCFLGDGECDEPEALG
AIGLAAREELDNLVFIVNCNLQRLDGPVRGNGKIIQELEGEFRGAGWEVVKVIWGRYWDPLLARDTSGKLLQLMEETVDG
EYQNCKAKGGAYTREHFFGKYPETAEMVANMSDDDIWRLNRGGHDPVKVYAALDHAQKTKGRPTVILAKTVKGYGLGDAG
EGKNIAHNVKKMGIESIRYFRDRFNIPIPDDQLEDLPFYHPGPDSEEVKYMMSRREALHGSVPQRRQKFTEELEIPSLKI
FDSILQGSNGREISSTMAFVRVLTALLKDKKIGKNIVPIIPDEARTFGMEGLFRQVGIYAHEGQKYIPQDSDQVAYYRED
KTGQVLQEGINELGAMSSWVSAATSYSVNNVPMIPFYIYYSMFGFQRIGDMAWAAGDMRARGFLVGGTSGRTTLNGEGLQ
HQDGHSHVLANTVPNCISYDPTYGYEIAVVVQDGIRRMYGENQEDIFYYLTTMNENYVQPEMPEGAEEGIVKGIYKLETV
SGSGKGKVQLMSCGTILEQTRKAAQALAKDFGITADVFSVTSFNELTRDGQAVERWNMLHPTQTPKQAYISQVISSDAPA
IAATDYMKIYGEQLRAYMPTDYKVLGTDGFGRSDSRENLRHHFEVDAKFIVIAALKSLVDRKELPVDVLANAIKEYGIDA
DKINPQYA

Sequences:

>Translated_888_residues
MSEDMLQDVDPLETQEWVDALQAVLEQEGPERAHFLLEKLIDKARRNGTHLPYNATTAYLNTIPAGQEPHMPGNQEMERR
IRAIIRWNALAMVLRGSKKDLELGGHISSFASSATIYDVCFNHFFRAPNEKDGGDLVYFQGHIAPGIYARSFLEGRLTED
QLANFRQEVDGKGLSSYPHPKLMPDYWQFPTVSMGLGPIQAIYQARFLKYLTDRGLKDCSAQTVYCFLGDGECDEPEALG
AIGLAAREELDNLVFIVNCNLQRLDGPVRGNGKIIQELEGEFRGAGWEVVKVIWGRYWDPLLARDTSGKLLQLMEETVDG
EYQNCKAKGGAYTREHFFGKYPETAEMVANMSDDDIWRLNRGGHDPVKVYAALDHAQKTKGRPTVILAKTVKGYGLGDAG
EGKNIAHNVKKMGIESIRYFRDRFNIPIPDDQLEDLPFYHPGPDSEEVKYMMSRREALHGSVPQRRQKFTEELEIPSLKI
FDSILQGSNGREISSTMAFVRVLTALLKDKKIGKNIVPIIPDEARTFGMEGLFRQVGIYAHEGQKYIPQDSDQVAYYRED
KTGQVLQEGINELGAMSSWVSAATSYSVNNVPMIPFYIYYSMFGFQRIGDMAWAAGDMRARGFLVGGTSGRTTLNGEGLQ
HQDGHSHVLANTVPNCISYDPTYGYEIAVVVQDGIRRMYGENQEDIFYYLTTMNENYVQPEMPEGAEEGIVKGIYKLETV
SGSGKGKVQLMSCGTILEQTRKAAQALAKDFGITADVFSVTSFNELTRDGQAVERWNMLHPTQTPKQAYISQVISSDAPA
IAATDYMKIYGEQLRAYMPTDYKVLGTDGFGRSDSRENLRHHFEVDAKFIVIAALKSLVDRKELPVDVLANAIKEYGIDA
DKINPQYA
>Mature_887_residues
SEDMLQDVDPLETQEWVDALQAVLEQEGPERAHFLLEKLIDKARRNGTHLPYNATTAYLNTIPAGQEPHMPGNQEMERRI
RAIIRWNALAMVLRGSKKDLELGGHISSFASSATIYDVCFNHFFRAPNEKDGGDLVYFQGHIAPGIYARSFLEGRLTEDQ
LANFRQEVDGKGLSSYPHPKLMPDYWQFPTVSMGLGPIQAIYQARFLKYLTDRGLKDCSAQTVYCFLGDGECDEPEALGA
IGLAAREELDNLVFIVNCNLQRLDGPVRGNGKIIQELEGEFRGAGWEVVKVIWGRYWDPLLARDTSGKLLQLMEETVDGE
YQNCKAKGGAYTREHFFGKYPETAEMVANMSDDDIWRLNRGGHDPVKVYAALDHAQKTKGRPTVILAKTVKGYGLGDAGE
GKNIAHNVKKMGIESIRYFRDRFNIPIPDDQLEDLPFYHPGPDSEEVKYMMSRREALHGSVPQRRQKFTEELEIPSLKIF
DSILQGSNGREISSTMAFVRVLTALLKDKKIGKNIVPIIPDEARTFGMEGLFRQVGIYAHEGQKYIPQDSDQVAYYREDK
TGQVLQEGINELGAMSSWVSAATSYSVNNVPMIPFYIYYSMFGFQRIGDMAWAAGDMRARGFLVGGTSGRTTLNGEGLQH
QDGHSHVLANTVPNCISYDPTYGYEIAVVVQDGIRRMYGENQEDIFYYLTTMNENYVQPEMPEGAEEGIVKGIYKLETVS
GSGKGKVQLMSCGTILEQTRKAAQALAKDFGITADVFSVTSFNELTRDGQAVERWNMLHPTQTPKQAYISQVISSDAPAI
AATDYMKIYGEQLRAYMPTDYKVLGTDGFGRSDSRENLRHHFEVDAKFIVIAALKSLVDRKELPVDVLANAIKEYGIDAD
KINPQYA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=890, Percent_Identity=66.7415730337079, Blast_Score=1283, Evalue=0.0,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 99521; Mature: 99390

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDMLQDVDPLETQEWVDALQAVLEQEGPERAHFLLEKLIDKARRNGTHLPYNATTAYL
CCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NTIPAGQEPHMPGNQEMERRIRAIIRWNALAMVLRGSKKDLELGGHISSFASSATIYDVC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHH
FNHFFRAPNEKDGGDLVYFQGHIAPGIYARSFLEGRLTEDQLANFRQEVDGKGLSSYPHP
HHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
KLMPDYWQFPTVSMGLGPIQAIYQARFLKYLTDRGLKDCSAQTVYCFLGDGECDEPEALG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHH
AIGLAAREELDNLVFIVNCNLQRLDGPVRGNGKIIQELEGEFRGAGWEVVKVIWGRYWDP
HHHHHHHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCH
LLARDTSGKLLQLMEETVDGEYQNCKAKGGAYTREHFFGKYPETAEMVANMSDDDIWRLN
HHHCCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCEEECC
RGGHDPVKVYAALDHAQKTKGRPTVILAKTVKGYGLGDAGEGKNIAHNVKKMGIESIRYF
CCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RDRFNIPIPDDQLEDLPFYHPGPDSEEVKYMMSRREALHGSVPQRRQKFTEELEIPSLKI
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
FDSILQGSNGREISSTMAFVRVLTALLKDKKIGKNIVPIIPDEARTFGMEGLFRQVGIYA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEE
HEGQKYIPQDSDQVAYYREDKTGQVLQEGINELGAMSSWVSAATSYSVNNVPMIPFYIYY
ECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SMFGFQRIGDMAWAAGDMRARGFLVGGTSGRTTLNGEGLQHQDGHSHVLANTVPNCISYD
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEHHHCCCCHHCCC
PTYGYEIAVVVQDGIRRMYGENQEDIFYYLTTMNENYVQPEMPEGAEEGIVKGIYKLETV
CCCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHEEEE
SGSGKGKVQLMSCGTILEQTRKAAQALAKDFGITADVFSVTSFNELTRDGQAVERWNMLH
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCC
PTQTPKQAYISQVISSDAPAIAATDYMKIYGEQLRAYMPTDYKVLGTDGFGRSDSRENLR
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHH
HHFEVDAKFIVIAALKSLVDRKELPVDVLANAIKEYGIDADKINPQYA
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
SEDMLQDVDPLETQEWVDALQAVLEQEGPERAHFLLEKLIDKARRNGTHLPYNATTAYL
CCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NTIPAGQEPHMPGNQEMERRIRAIIRWNALAMVLRGSKKDLELGGHISSFASSATIYDVC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHH
FNHFFRAPNEKDGGDLVYFQGHIAPGIYARSFLEGRLTEDQLANFRQEVDGKGLSSYPHP
HHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
KLMPDYWQFPTVSMGLGPIQAIYQARFLKYLTDRGLKDCSAQTVYCFLGDGECDEPEALG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHH
AIGLAAREELDNLVFIVNCNLQRLDGPVRGNGKIIQELEGEFRGAGWEVVKVIWGRYWDP
HHHHHHHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCH
LLARDTSGKLLQLMEETVDGEYQNCKAKGGAYTREHFFGKYPETAEMVANMSDDDIWRLN
HHHCCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCEEECC
RGGHDPVKVYAALDHAQKTKGRPTVILAKTVKGYGLGDAGEGKNIAHNVKKMGIESIRYF
CCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RDRFNIPIPDDQLEDLPFYHPGPDSEEVKYMMSRREALHGSVPQRRQKFTEELEIPSLKI
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
FDSILQGSNGREISSTMAFVRVLTALLKDKKIGKNIVPIIPDEARTFGMEGLFRQVGIYA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEE
HEGQKYIPQDSDQVAYYREDKTGQVLQEGINELGAMSSWVSAATSYSVNNVPMIPFYIYY
ECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SMFGFQRIGDMAWAAGDMRARGFLVGGTSGRTTLNGEGLQHQDGHSHVLANTVPNCISYD
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEHHHCCCCHHCCC
PTYGYEIAVVVQDGIRRMYGENQEDIFYYLTTMNENYVQPEMPEGAEEGIVKGIYKLETV
CCCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHEEEE
SGSGKGKVQLMSCGTILEQTRKAAQALAKDFGITADVFSVTSFNELTRDGQAVERWNMLH
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCC
PTQTPKQAYISQVISSDAPAIAATDYMKIYGEQLRAYMPTDYKVLGTDGFGRSDSRENLR
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHH
HHFEVDAKFIVIAALKSLVDRKELPVDVLANAIKEYGIDADKINPQYA
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]