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The map label for this gene is murD

Identifier: 120597219

GI number: 120597219

Start: 419467

End: 420786

Strand: Direct

Name: murD

Synonym: Sputw3181_0388

Alternate gene names: 120597219

Gene position: 419467-420786 (Clockwise)

Preceding gene: 120597218

Following gene: 120597220

Centisome position: 8.91

GC content: 49.77

Gene sequence:

>1320_bases
ATGCAACGTCAGTATTCACACATAGTGTTAGGTTTGGGCGCAACGGGGTTGTCCGTTGTACGCTATTTGTGTGGAAAGGG
CATTACGCCACTGGTGATGGATAGCCGTAGACAACCTCCGGGGGCGGATACTCTTGCGGCCTCTTTCCCTGAAGTACAGT
TAATTACGGGTGGTTTTGATTGTCGCTATTTAGTACAAGCCAGCCAAATTATTATTAGCCCAGGCATTGCTCTCGATACC
GCTGAAGTGCGTGCAGCGTTAGATATGGGAATAGAGGTCATTGGTGACGTTGAACTTTTTGCCAGAGAAATCGCAGACCG
AAAACCCTGCGTGATTGGTATTACTGGCTCTAATGGCAAGTCGACGGTCACAACCTTAGTCGGTGAAATGCTGCGTGAAG
CAGGCATGGCTGTCGCTGTGGGGGGCAATATTGGTGTTCCTGCACTAGATCTGCTGAGCGAGAATGCAGAGATTTTTGTG
CTTGAGCTGTCGAGCTTCCAACTGGAAACCACTCACAGCTTGAATTGCGTGGCTTCAACTTGCTTGAACGTGACGGAAGA
TCACATGGACAGATACAGCGATATGGATGCCTATCGCAAAGCAAAGCTGCGTCTGTACCATCAAAGTCGCTCCATCATTT
TTAACCGTGATGATGCGCTGACTATACCTACCGAACCTATGAACCAAAACAGCTTTGGTTTAGCGCCGCCAGAGGGCGAC
GAGTGGGGTATTTGTGATAGTAAGATTTATCATGGTCGCAGCGAAATTATGCCAATTACCGAAGTGTCGCTAATTGGCAG
TCATAACCATGCCAACTTACTTGCAGCCATGGCTCTAGTCTATGCCGTGGGTGTAGATAAGCAAGTGATGGCGAAGGTAG
CTCGCACCTTTACTGGGCTCTCACACCGCTGTGAAGTGGTTGGGATAAAAGGCGGTGTGACTTATGTGAACGATTCCAAG
GCCACCAATGTGGGCGCAACAGTTGCCGCACTTGATGGTTTAAGCGATCACTTAGGCGACATTATTTTAATTGCTGGCGG
TGATGGCAAGGGGGCGGATTTCAGCCCGCTGGTTGAGCCACTGAGCAAGGTGACTCATCTGATTACCTTAGGTCGTGATG
GCAATAAGATTGCTGCGCTGAAGGAAGGGGCTATTAAAGTCGACTCTATGGCTGCAGCCGTTGCCAAGGCGGCTGCATTA
GCCACCTCAGGCGATATCGTTTTATTATCTCCCGCCTGTGCGAGTCTAGATATGTACAGTAACTTTATGGCGCGTGGCGA
TGATTTCCGCAATCTGGTGGAGCTGCTCGATGGCGAGTGA

Upstream 100 bases:

>100_bases
GAAAGGTTGGCCTGAACCTCGAGTGATAGTGCGTTTTTGGATTATCTCGATATTCCTCGTTTTGCTCGGTTTAGCCACAT
TAAAGTTAAGGTAATAGGGT

Downstream 100 bases:

>100_bases
TACAAGGCAATTCAGCCTGTTTGGTCGCTTGCTATCCGCAATGCCAAACTGGCAACGTGATACTGAAGTGCCGGGCGTGC
AATTGTACGACCGTGCTTTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MQRQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGADTLAASFPEVQLITGGFDCRYLVQASQIIISPGIALDT
AEVRAALDMGIEVIGDVELFAREIADRKPCVIGITGSNGKSTVTTLVGEMLREAGMAVAVGGNIGVPALDLLSENAEIFV
LELSSFQLETTHSLNCVASTCLNVTEDHMDRYSDMDAYRKAKLRLYHQSRSIIFNRDDALTIPTEPMNQNSFGLAPPEGD
EWGICDSKIYHGRSEIMPITEVSLIGSHNHANLLAAMALVYAVGVDKQVMAKVARTFTGLSHRCEVVGIKGGVTYVNDSK
ATNVGATVAALDGLSDHLGDIILIAGGDGKGADFSPLVEPLSKVTHLITLGRDGNKIAALKEGAIKVDSMAAAVAKAAAL
ATSGDIVLLSPACASLDMYSNFMARGDDFRNLVELLDGE

Sequences:

>Translated_439_residues
MQRQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGADTLAASFPEVQLITGGFDCRYLVQASQIIISPGIALDT
AEVRAALDMGIEVIGDVELFAREIADRKPCVIGITGSNGKSTVTTLVGEMLREAGMAVAVGGNIGVPALDLLSENAEIFV
LELSSFQLETTHSLNCVASTCLNVTEDHMDRYSDMDAYRKAKLRLYHQSRSIIFNRDDALTIPTEPMNQNSFGLAPPEGD
EWGICDSKIYHGRSEIMPITEVSLIGSHNHANLLAAMALVYAVGVDKQVMAKVARTFTGLSHRCEVVGIKGGVTYVNDSK
ATNVGATVAALDGLSDHLGDIILIAGGDGKGADFSPLVEPLSKVTHLITLGRDGNKIAALKEGAIKVDSMAAAVAKAAAL
ATSGDIVLLSPACASLDMYSNFMARGDDFRNLVELLDGE
>Mature_439_residues
MQRQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGADTLAASFPEVQLITGGFDCRYLVQASQIIISPGIALDT
AEVRAALDMGIEVIGDVELFAREIADRKPCVIGITGSNGKSTVTTLVGEMLREAGMAVAVGGNIGVPALDLLSENAEIFV
LELSSFQLETTHSLNCVASTCLNVTEDHMDRYSDMDAYRKAKLRLYHQSRSIIFNRDDALTIPTEPMNQNSFGLAPPEGD
EWGICDSKIYHGRSEIMPITEVSLIGSHNHANLLAAMALVYAVGVDKQVMAKVARTFTGLSHRCEVVGIKGGVTYVNDSK
ATNVGATVAALDGLSDHLGDIILIAGGDGKGADFSPLVEPLSKVTHLITLGRDGNKIAALKEGAIKVDSMAAAVAKAAAL
ATSGDIVLLSPACASLDMYSNFMARGDDFRNLVELLDGE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=443, Percent_Identity=48.7584650112867, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46509; Mature: 46509

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGADTLAASFPEVQLITGGFD
CCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHCCCCCEEEEECCCC
CRYLVQASQIIISPGIALDTAEVRAALDMGIEVIGDVELFAREIADRKPCVIGITGSNGK
EEEEEEECEEEEECCCEECHHHHHHHHHCCHHEECHHHHHHHHHCCCCCEEEEEECCCCC
STVTTLVGEMLREAGMAVAVGGNIGVPALDLLSENAEIFVLELSSFQLETTHSLNCVAST
HHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEEECCEEEECCCCHHHHHHH
CLNVTEDHMDRYSDMDAYRKAKLRLYHQSRSIIFNRDDALTIPTEPMNQNSFGLAPPEGD
HHCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCEEECCCCCCCCCCCCCCCCCCC
EWGICDSKIYHGRSEIMPITEVSLIGSHNHANLLAAMALVYAVGVDKQVMAKVARTFTGL
CCCCCCCHHHCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SHRCEVVGIKGGVTYVNDSKATNVGATVAALDGLSDHLGDIILIAGGDGKGADFSPLVEP
CCCEEEEEECCCEEEECCCCCCCCCHHHHHHCCCHHCCCCEEEEECCCCCCCCHHHHHHH
LSKVTHLITLGRDGNKIAALKEGAIKVDSMAAAVAKAAALATSGDIVLLSPACASLDMYS
HHHHHHEEEECCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
NFMARGDDFRNLVELLDGE
HHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQRQYSHIVLGLGATGLSVVRYLCGKGITPLVMDSRRQPPGADTLAASFPEVQLITGGFD
CCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHCCCCCEEEEECCCC
CRYLVQASQIIISPGIALDTAEVRAALDMGIEVIGDVELFAREIADRKPCVIGITGSNGK
EEEEEEECEEEEECCCEECHHHHHHHHHCCHHEECHHHHHHHHHCCCCCEEEEEECCCCC
STVTTLVGEMLREAGMAVAVGGNIGVPALDLLSENAEIFVLELSSFQLETTHSLNCVAST
HHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEEECCEEEECCCCHHHHHHH
CLNVTEDHMDRYSDMDAYRKAKLRLYHQSRSIIFNRDDALTIPTEPMNQNSFGLAPPEGD
HHCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCEEECCCCCCCCCCCCCCCCCCC
EWGICDSKIYHGRSEIMPITEVSLIGSHNHANLLAAMALVYAVGVDKQVMAKVARTFTGL
CCCCCCCHHHCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SHRCEVVGIKGGVTYVNDSKATNVGATVAALDGLSDHLGDIILIAGGDGKGADFSPLVEP
CCCEEEEEECCCEEEECCCCCCCCCHHHHHHCCCHHCCCCEEEEECCCCCCCCHHHHHHH
LSKVTHLITLGRDGNKIAALKEGAIKVDSMAAAVAKAAALATSGDIVLLSPACASLDMYS
HHHHHHEEEECCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
NFMARGDDFRNLVELLDGE
HHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]