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The map label for this gene is leuA [H]

Identifier: 120597205

GI number: 120597205

Start: 402754

End: 404322

Strand: Direct

Name: leuA [H]

Synonym: Sputw3181_0374

Alternate gene names: 120597205

Gene position: 402754-404322 (Clockwise)

Preceding gene: 120597203

Following gene: 120597206

Centisome position: 8.55

GC content: 48.25

Gene sequence:

>1569_bases
ATGTCTAACAGAGTCATAATATTTGATACTACCTTGCGTGATGGTGAGCAGGCGTTAGCCGCAAGTTTGTCGGTCAAAGA
AAAACTACAGATCGCTATGGCGCTGGAACGTCTCGGTGTGGATGTGATGGAAGTGGGTTTTCCGGTTTCTTCACCCGGTG
ATTTTGAGTCAGTGCAGACCATAGCGCGTACCATTAAAAATAGCCGCGTTTGTGCCTTATCCCGTGCATTGGAAAAGGAC
ATCGATGCCGCGGCGCAGGCCTTGTCTGTCGCAGATCAATTCCGTATCCATACGTTTATCTCGACCTCAACGATTCACGT
TGAGAGCAAGTTGAAGCGTTCTTTCGATCAAGTGTTAGAGATGGCGGTGGGCGCAGTGAAGTATGCCCGCCGTTTTACTG
ATGACGTGGAGTTTTCCTGTGAGGATGCGGGGCGTACGCCCATCGATAATCTATGTCGCATGGTAGAGGCCGCCATTCTT
GCTGGCGCGCGCACCATTAATATTCCCGATACGGTCGGTTACACAGTACCAAGCGAGTTTGGCAATATCATTCAGACTTT
GTTTAATCGGGTGCCGAATATCGACCAAGCGGTGATCTCAGTGCATTGCCACGATGACTTAGGTTTATCTGTGGCTAACT
CGATTACCGCTGTACAGCACGGTGCGCGTCAAATTGAATGTACCATCAATGGCATAGGTGAGCGTGCGGGTAACTGTTCA
CTGGAAGAGATCGCGATGATCTTAGCGACGCGTAAGGGAATGTTAGGGCTAGAAACAGGCATTAATGCTAAAGAAATTCA
CCGTACTTCTAATTTGGTCAGCCAGTTATGCAATATGCCAGTGCAGGCGAACAAAGCAATTGTCGGTGCTAATGCTTTTA
CTCATTCGTCAGGTATCCATCAGGATGGCATGTTAAAAGCACAAAATACCTATGAAATCATGACGCCAGAAAGCATAGGT
TTGAATCGCAATAACTTGAATATGACCTCTCGCTCGGGTCGCCATGTGATCAAGCATCGCATGGAAGAAATGGGCTATAG
CGAGCACGATTACAATATGGATACTCTGTATGAAGAGTTTCTAAAATTGGCAGATAAAAAAGGCCAAGTATTTGATTATG
ATTTAGAAGCCTTAGCCTTTATGGAAGCGCAGGCCGAAGAAGACAATCACTATCAATTGCAGCAATTAGTGGTGCAGTCT
GACTCAACTGAAGGCGTTGCTACGGCGACTGTGCGTATCGAAGTGGGCGGCGAAATTAAAACCGAAGCCGCAACGGGTAA
TGGTCCTGTTGATGCTGCCTATAACGCGATTGCCCGCGCAACCGATCGTCGAATCGATATCATCAGCTACAAGCTAGGCG
CTAAGGGCGTAGGTCAAAATGCATTAGGCCAAGTGGACATTACCGCGGTTTACCACGAGCAGAACTTTCACGGCGTAGGT
TTAGCGACCGATGTGGTCGAAGCCTCGGCGCGCGCTTTAGTGCATGTGATGAACTTAACGTGCCGCGCAGACAAGGTCGC
CGATTACAAACAAAGCATGCAAAAAAATCGAGAGCTAGGCGGCGTCTAG

Upstream 100 bases:

>100_bases
GCAAAGCATTCACAAACCCCGCACAAATGTTGCGGGGTTTTTTGTATCTGAATACGGCAAAAATTAACTAACGAACGGTG
ACGTTCCATGGAGAAGCGAG

Downstream 100 bases:

>100_bases
TCGCTAAAAGCATCCAAAGAATAGCAAGGTCGAGCATAAAGCATTTACATTTAAAGGAGTTGGCTGGCGTATGAGTTATC
AAATAGCAGTATTAGCAGGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSNRVIIFDTTLRDGEQALAASLSVKEKLQIAMALERLGVDVMEVGFPVSSPGDFESVQTIARTIKNSRVCALSRALEKD
IDAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLEMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAIL
AGARTINIPDTVGYTVPSEFGNIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTINGIGERAGNCS
LEEIAMILATRKGMLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMEEMGYSEHDYNMDTLYEEFLKLADKKGQVFDYDLEALAFMEAQAEEDNHYQLQQLVVQS
DSTEGVATATVRIEVGGEIKTEAATGNGPVDAAYNAIARATDRRIDIISYKLGAKGVGQNALGQVDITAVYHEQNFHGVG
LATDVVEASARALVHVMNLTCRADKVADYKQSMQKNRELGGV

Sequences:

>Translated_522_residues
MSNRVIIFDTTLRDGEQALAASLSVKEKLQIAMALERLGVDVMEVGFPVSSPGDFESVQTIARTIKNSRVCALSRALEKD
IDAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLEMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAIL
AGARTINIPDTVGYTVPSEFGNIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTINGIGERAGNCS
LEEIAMILATRKGMLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMEEMGYSEHDYNMDTLYEEFLKLADKKGQVFDYDLEALAFMEAQAEEDNHYQLQQLVVQS
DSTEGVATATVRIEVGGEIKTEAATGNGPVDAAYNAIARATDRRIDIISYKLGAKGVGQNALGQVDITAVYHEQNFHGVG
LATDVVEASARALVHVMNLTCRADKVADYKQSMQKNRELGGV
>Mature_521_residues
SNRVIIFDTTLRDGEQALAASLSVKEKLQIAMALERLGVDVMEVGFPVSSPGDFESVQTIARTIKNSRVCALSRALEKDI
DAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLEMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAILA
GARTINIPDTVGYTVPSEFGNIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTINGIGERAGNCSL
EEIAMILATRKGMLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIGL
NRNNLNMTSRSGRHVIKHRMEEMGYSEHDYNMDTLYEEFLKLADKKGQVFDYDLEALAFMEAQAEEDNHYQLQQLVVQSD
STEGVATATVRIEVGGEIKTEAATGNGPVDAAYNAIARATDRRIDIISYKLGAKGVGQNALGQVDITAVYHEQNFHGVGL
ATDVVEASARALVHVMNLTCRADKVADYKQSMQKNRELGGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=295, Percent_Identity=23.3898305084746, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786261, Length=518, Percent_Identity=66.6023166023166, Blast_Score=714, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324225, Length=536, Percent_Identity=26.4925373134328, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320019, Length=365, Percent_Identity=27.6712328767123, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=361, Percent_Identity=27.4238227146814, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=543, Percent_Identity=24.3093922651934, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24582381, Length=279, Percent_Identity=24.7311827956989, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56940; Mature: 56809

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRVIIFDTTLRDGEQALAASLSVKEKLQIAMALERLGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARTIKNSRVCALSRALEKDIDAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAILAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GNIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILATRKGMLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIH
HHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEECCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMEEMGYSEHDYNMDTLYEEF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFMEAQAEEDNHYQLQQLVVQSDSTEGVATATVRIEVGGEIK
HHHHCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCEE
TEAATGNGPVDAAYNAIARATDRRIDIISYKLGAKGVGQNALGQVDITAVYHEQNFHGVG
ECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCC
LATDVVEASARALVHVMNLTCRADKVADYKQSMQKNRELGGV
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNRVIIFDTTLRDGEQALAASLSVKEKLQIAMALERLGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARTIKNSRVCALSRALEKDIDAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAILAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GNIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILATRKGMLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIH
HHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEECCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMEEMGYSEHDYNMDTLYEEF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFMEAQAEEDNHYQLQQLVVQSDSTEGVATATVRIEVGGEIK
HHHHCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCEE
TEAATGNGPVDAAYNAIARATDRRIDIISYKLGAKGVGQNALGQVDITAVYHEQNFHGVG
ECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCC
LATDVVEASARALVHVMNLTCRADKVADYKQSMQKNRELGGV
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA