Click here to switch to the map view.

The map label for this gene is leuB [H]

Identifier: 120597206

GI number: 120597206

Start: 404393

End: 405487

Strand: Direct

Name: leuB [H]

Synonym: Sputw3181_0375

Alternate gene names: 120597206

Gene position: 404393-405487 (Clockwise)

Preceding gene: 120597205

Following gene: 120597207

Centisome position: 8.59

GC content: 50.14

Gene sequence:

>1095_bases
ATGAGTTATCAAATAGCAGTATTAGCAGGGGATGGGATCGGTCCTGAAGTGATGGCAGAGGCGCGTAAAGTGCTGCGTGA
AGTGGAAGCGCGATTCGATTTAAATATCGAATACACTGAATATGATGTTGGCGGTATTGCCATTGATAACCACGGTTGTC
CCTTACCCGATGCCACGCTAAAGGGCTGTGAAGCGGCCGATGCGATCCTATTTGGCTCGGTAGGCGGCCCTAAGTGGGAA
AAGTTACCGCCAAATGAGCAACCTGAGCGCGGTGCACTACTGCCCTTGCGTGGTCACTTCGAACTGTTTTGTAATCTGCG
CCCAGCTAAATTGCATGATGGCTTAGAACATATGTCACCGCTGCGTAGCGATATCTCTGCCCGTGGATTTGATGTCTTAT
GTGTACGCGAGTTAACTGGCGGCATTTATTTTGGTAAGCCAAAGGGTCGTCAAGGTGAAGGCGAAAATGAAGAAGCCTTC
GATACCATGCGTTATAGCCGTCGCGAAATTAGCCGTATCGCCCGCATAGCCTTTGAAGCCGCCCGTGGTCGCCGTAAAAA
AGTGACCTCAGTGGATAAAGCCAACGTGCTCGCCTGTTCAGTGTTATGGCGCCAAGTGGTTGAAGAAGTCGCGGTAGATT
TCCCCGATGTCGAGCTTGAACACATCTATATCGATAATGCGACCATGCAGTTGCTACGTCGTCCCGATGAGTTCGATGTG
ATGCTATGTTCTAACCTGTTCGGCGATATTTTATCCGACGAAATCGCCATGTTAACGGGCTCTATGGGCTTGTTGTCATC
GGCGAGCATGAACAGCACTGGCTTTGGTTTATTCGAACCTGCGGGTGGCAGTGCGCCGGATATCGCAGGTAAAGGCATTG
CAAACCCTATCGCCCAAATTCTGTCGGCGGCATTAATGCTGCGCCACAGCTTAAAGCAAGAAGAAGCCGCCTGTGCGATT
GAACGCGCCGTAAGCAAAGCATTAAATTCTGGCTACTTAACTGGTGAGCTATTGAGCAGCGACAAGCGTAGCCAAGCTAA
ATCAACCGCCGAGATGGGTGACTTTATCGCCAATGCTATAAAGGAAGGTGTGTAA

Upstream 100 bases:

>100_bases
CAAAAAAATCGAGAGCTAGGCGGCGTCTAGTCGCTAAAAGCATCCAAAGAATAGCAAGGTCGAGCATAAAGCATTTACAT
TTAAAGGAGTTGGCTGGCGT

Downstream 100 bases:

>100_bases
TGACGACTGTGCCAAAAAACGCGGTGGCAAAAACGCTGTATCAAAAAGTATGGGATGCCCACGTTGTAGCGACTCCTGAA
GGTGAAGCGCCGATCATTTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSYQIAVLAGDGIGPEVMAEARKVLREVEARFDLNIEYTEYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
KLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSPLRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAF
DTMRYSRREISRIARIAFEAARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSTGFGLFEPAGGSAPDIAGKGIANPIAQILSAALMLRHSLKQEEAACAI
ERAVSKALNSGYLTGELLSSDKRSQAKSTAEMGDFIANAIKEGV

Sequences:

>Translated_364_residues
MSYQIAVLAGDGIGPEVMAEARKVLREVEARFDLNIEYTEYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
KLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSPLRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAF
DTMRYSRREISRIARIAFEAARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSTGFGLFEPAGGSAPDIAGKGIANPIAQILSAALMLRHSLKQEEAACAI
ERAVSKALNSGYLTGELLSSDKRSQAKSTAEMGDFIANAIKEGV
>Mature_363_residues
SYQIAVLAGDGIGPEVMAEARKVLREVEARFDLNIEYTEYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWEK
LPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSPLRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAFD
TMRYSRREISRIARIAFEAARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDVM
LCSNLFGDILSDEIAMLTGSMGLLSSASMNSTGFGLFEPAGGSAPDIAGKGIANPIAQILSAALMLRHSLKQEEAACAIE
RAVSKALNSGYLTGELLSSDKRSQAKSTAEMGDFIANAIKEGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=326, Percent_Identity=32.5153374233129, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI4758582, Length=335, Percent_Identity=29.8507462686567, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=29.8507462686567, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI28178816, Length=316, Percent_Identity=30.0632911392405, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI28178821, Length=316, Percent_Identity=30.0632911392405, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178819, Length=148, Percent_Identity=40.5405405405405, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=69.4214876033058, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1788101, Length=312, Percent_Identity=38.7820512820513, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=25.6218905472637, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=31.9648093841642, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=330, Percent_Identity=30.9090909090909, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.3977272727273, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=319, Percent_Identity=32.6018808777429, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=44.3243243243243, Blast_Score=272, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=344, Percent_Identity=35.4651162790698, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324709, Length=340, Percent_Identity=31.7647058823529, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=347, Percent_Identity=29.3948126801153, Blast_Score=122, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=35.0877192982456, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=35.0877192982456, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI161078637, Length=333, Percent_Identity=30.9309309309309, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078635, Length=333, Percent_Identity=30.9309309309309, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078633, Length=333, Percent_Identity=30.9309309309309, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24650122, Length=333, Percent_Identity=30.9309309309309, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078639, Length=329, Percent_Identity=31.306990881459, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24661184, Length=332, Percent_Identity=28.9156626506024, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=26.1363636363636, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=26.1363636363636, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20130355, Length=354, Percent_Identity=24.8587570621469, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39508; Mature: 39376

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQIAVLAGDGIGPEVMAEARKVLREVEARFDLNIEYTEYDVGGIAIDNHGCPLPDATL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEKLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSP
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHH
LRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAFDTMRYSRREISRIARIAFEA
HHHHHHCCCCEEEEEEHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSTGFGLFEPAGGSAPDIAGKGIANPIAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
LSAALMLRHSLKQEEAACAIERAVSKALNSGYLTGELLSSDKRSQAKSTAEMGDFIANAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHH
KEGV
HHCC
>Mature Secondary Structure 
SYQIAVLAGDGIGPEVMAEARKVLREVEARFDLNIEYTEYDVGGIAIDNHGCPLPDATL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEKLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSP
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHH
LRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAFDTMRYSRREISRIARIAFEA
HHHHHHCCCCEEEEEEHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSTGFGLFEPAGGSAPDIAGKGIANPIAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
LSAALMLRHSLKQEEAACAIERAVSKALNSGYLTGELLSSDKRSQAKSTAEMGDFIANAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHH
KEGV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]