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The map label for this gene is insF1 [H]

Identifier: 120597191

GI number: 120597191

Start: 386616

End: 386975

Strand: Direct

Name: insF1 [H]

Synonym: Sputw3181_0360

Alternate gene names: 120597191

Gene position: 386616-386975 (Clockwise)

Preceding gene: 120597190

Following gene: 120597192

Centisome position: 8.21

GC content: 41.67

Gene sequence:

>360_bases
ATGGCATTTGAATCACGGGGTAAGCCTAAAAACCTGATATTTCACTCAGACCAAGGCAGTCATTACACTAGCCTTAAATA
CCGTCAACGGCTGTGGAAATATCAAATAACCCAGAGCATGAGTCGCCGAGGTAACTGCTGGGATAATGCGCCAATGGAGC
GATTTTTCAGGAGTTTAAAAACGGAATGGGTGCCAACGCAAGGCTATAAGAGTTTTATTGAAGCTAAACAAGATGTGACT
GATTATATTATTGGCTATTACAGCCAAGTCAGGCCACATCATTATAATGCAGGATTAAGCCCAAATGAATCAGAGCGAAG
ATTTTGGGAAGATTCTAAAGCCGTGGCCAAATTTAGTTGA

Upstream 100 bases:

>100_bases
GGTGTTATTTAGCGGTTGTGCTGGACTTATTTTCCAGAAAAGCGGTGGGTTGGGCAATGTCATTTTCACCAGATACTGAG
TTAACAGCAAAGCACTGGAA

Downstream 100 bases:

>100_bases
CCACTTCAAACATCAATACAGGTGTATTGAATGGTAACTTCAATCTCAATCAATGAGATAGGTAAAGTAAAGGTATGACA
GGAACGTTAGTTCAAATCCA

Product: transposase OrfAB subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 118

Protein sequence:

>119_residues
MAFESRGKPKNLIFHSDQGSHYTSLKYRQRLWKYQITQSMSRRGNCWDNAPMERFFRSLKTEWVPTQGYKSFIEAKQDVT
DYIIGYYSQVRPHHYNAGLSPNESERRFWEDSKAVAKFS

Sequences:

>Translated_119_residues
MAFESRGKPKNLIFHSDQGSHYTSLKYRQRLWKYQITQSMSRRGNCWDNAPMERFFRSLKTEWVPTQGYKSFIEAKQDVT
DYIIGYYSQVRPHHYNAGLSPNESERRFWEDSKAVAKFS
>Mature_118_residues
AFESRGKPKNLIFHSDQGSHYTSLKYRQRLWKYQITQSMSRRGNCWDNAPMERFFRSLKTEWVPTQGYKSFIEAKQDVTD
YIIGYYSQVRPHHYNAGLSPNESERRFWEDSKAVAKFS

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1787263, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1786753, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1786570, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1786490, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1789981, Length=102, Percent_Identity=40.1960784313725, Blast_Score=69, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 14226; Mature: 14095

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFESRGKPKNLIFHSDQGSHYTSLKYRQRLWKYQITQSMSRRGNCWDNAPMERFFRSLK
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TEWVPTQGYKSFIEAKQDVTDYIIGYYSQVRPHHYNAGLSPNESERRFWEDSKAVAKFS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
AFESRGKPKNLIFHSDQGSHYTSLKYRQRLWKYQITQSMSRRGNCWDNAPMERFFRSLK
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TEWVPTQGYKSFIEAKQDVTDYIIGYYSQVRPHHYNAGLSPNESERRFWEDSKAVAKFS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]