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The map label for this gene is pyrE

Identifier: 120597164

GI number: 120597164

Start: 360610

End: 361251

Strand: Direct

Name: pyrE

Synonym: Sputw3181_0333

Alternate gene names: 120597164

Gene position: 360610-361251 (Clockwise)

Preceding gene: 120597163

Following gene: 120597169

Centisome position: 7.66

GC content: 49.07

Gene sequence:

>642_bases
GTGAAAGCCTATCAACGTGAGTTTATTGAATTTGCCTTAGAGCGTCAGGTACTGCGATTCGGCGAGTTTACCCTTAAATC
GGGCCGTATCAGTCCTTATTTCTTCAATGCGGGTTTGTTCAATACCGGCCGAGATTTAGCGCGTCTTGGCCGTTTTTATG
CCGCTGCGTTAGTGGATTCTGGCATTGATTATGACTTGCTGTTTGGTCCCGCTTACAAAGGCATTCCGATTGCGACGACT
ACGGCGGTTGCCTTGTGTGAGCATCACGATATCGACATCCCTTACTGCTTTAATCGCAAAGAAAAGAAAGATCACGGCGA
AGGTGGCAGCTTAGTCGGTAGCGAGCTAAAAGGCCGTGTGATGCTGGTGGACGATGTGATTACTGCGGGTACTGCGATTC
GTGAGTCGATGGAAATCATCGAAGCCCATAAAGCGCAATTAGCCGGCGTGTTGATTGCCTTAGACAGGCAAGAGAAAGGT
AAGGGCGAGTTATCGGCTATCCAAGAGGTTGAGCGTGATTTTGGTTGCGGTATTGTGGCGATTATTAAACTCGCCGATCT
GATCAGCTATCTGTCTGAAAAACCAGGGATGGAAGTGCAGCTTGCTGCCGTGAGCCAGTACCGCGAGCAATACGGGATTT
AA

Upstream 100 bases:

>100_bases
TGTAACAAGAGGCTAGCGTGATCCAACCCTGTACCTAATAAAATTTTATAAAATATTTCCTAATAAAAACTAGTTACATA
CAAAGTCGTAAGGAGAGATT

Downstream 100 bases:

>100_bases
GAGGAACCTAGGCTCGAGAGTCTAGGCTCTAGAATCTAGAACCTAGGCCCTCTATGTCTTATTGATGCAAAAACTCGGCG
CGAGTTGCTGGGTTAGATTT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKAYQREFIEFALERQVLRFGEFTLKSGRISPYFFNAGLFNTGRDLARLGRFYAAALVDSGIDYDLLFGPAYKGIPIATT
TAVALCEHHDIDIPYCFNRKEKKDHGEGGSLVGSELKGRVMLVDDVITAGTAIRESMEIIEAHKAQLAGVLIALDRQEKG
KGELSAIQEVERDFGCGIVAIIKLADLISYLSEKPGMEVQLAAVSQYREQYGI

Sequences:

>Translated_213_residues
MKAYQREFIEFALERQVLRFGEFTLKSGRISPYFFNAGLFNTGRDLARLGRFYAAALVDSGIDYDLLFGPAYKGIPIATT
TAVALCEHHDIDIPYCFNRKEKKDHGEGGSLVGSELKGRVMLVDDVITAGTAIRESMEIIEAHKAQLAGVLIALDRQEKG
KGELSAIQEVERDFGCGIVAIIKLADLISYLSEKPGMEVQLAAVSQYREQYGI
>Mature_213_residues
MKAYQREFIEFALERQVLRFGEFTLKSGRISPYFFNAGLFNTGRDLARLGRFYAAALVDSGIDYDLLFGPAYKGIPIATT
TAVALCEHHDIDIPYCFNRKEKKDHGEGGSLVGSELKGRVMLVDDVITAGTAIRESMEIIEAHKAQLAGVLIALDRQEKG
KGELSAIQEVERDFGCGIVAIIKLADLISYLSEKPGMEVQLAAVSQYREQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=153, Percent_Identity=29.4117647058824, Blast_Score=81, Evalue=7e-16,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=79.3427230046948, Blast_Score=356, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17508631, Length=163, Percent_Identity=28.8343558282209, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=49.0909090909091, Blast_Score=205, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=44.3438914027149, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23558; Mature: 23558

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYQREFIEFALERQVLRFGEFTLKSGRISPYFFNAGLFNTGRDLARLGRFYAAALVDS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIDYDLLFGPAYKGIPIATTTAVALCEHHDIDIPYCFNRKEKKDHGEGGSLVGSELKGRV
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCE
MLVDDVITAGTAIRESMEIIEAHKAQLAGVLIALDRQEKGKGELSAIQEVERDFGCGIVA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCHHHH
IIKLADLISYLSEKPGMEVQLAAVSQYREQYGI
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKAYQREFIEFALERQVLRFGEFTLKSGRISPYFFNAGLFNTGRDLARLGRFYAAALVDS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIDYDLLFGPAYKGIPIATTTAVALCEHHDIDIPYCFNRKEKKDHGEGGSLVGSELKGRV
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCE
MLVDDVITAGTAIRESMEIIEAHKAQLAGVLIALDRQEKGKGELSAIQEVERDFGCGIVA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCHHHH
IIKLADLISYLSEKPGMEVQLAAVSQYREQYGI
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]