Click here to switch to the map view.

The map label for this gene is saeS [H]

Identifier: 120597083

GI number: 120597083

Start: 274994

End: 276250

Strand: Direct

Name: saeS [H]

Synonym: Sputw3181_0252

Alternate gene names: 120597083

Gene position: 274994-276250 (Clockwise)

Preceding gene: 120597082

Following gene: 120597084

Centisome position: 5.84

GC content: 46.06

Gene sequence:

>1257_bases
GTGACTGTGATTATAGGTGCGTTGTTATTTGCGCTTTATCGACAACTGATCATCGAGCAGCAGTATCAAGTCACTCAGTA
TCTTGAGTCTGAAACCCAACGCTATCAGCAGCTTGCCCTTACTGTTGATCGCCGTAGTTTTGCGGCTCAAATCCGCGCCG
CCGATCCACAAACCGCTCTGATCGCTTGGCGTAATAGTTACGATATGGTCGGTGCCTTGAGCTTTATGCCCGAAGGCATG
CCCATGCTGCCGCAAACGCGGGATTTTCCTATCTTAACTGGTGGCCCGGATAAACTGCATATCCTCACTGGCGGTCTGGT
GATGACCCGCTACGGCCCAGTGTTGATTGCGACTCGCACCGATAACTTAGCCACATTAATCGACAAATTTATCAGTGCTG
CTGCAACCGCGGTGATGTTGACTATAGTATTAACCTTAGCGTTGGGCTATCTGTTTTCTAAGGCCATTTTGCGTCGGCTT
GTGCAGTACAACCGATTGAGTGAACAGATTGAGCGCGGTCATTACGATACGCGTTTACCGCTAAGTTGGCGTCAGGATGA
GTTTGATATGTTGGCGCTGCAATTTAATAATGTGCTGGATATTCTCGAAAATAATCTGATGGCAGTGCGCGGAGCCACTG
ACAATATTGCCCATGATTTACGTACACCGTTATCCCATATCCGTATTGGGCTAGAAGAATTGGCCACTAAGTCACATGAT
GATATCAGCGAAGGTTGTGCCATTTTAATTGAGGAGCTTGATCATTGTTTGGCGACATTTGATGCTATGTTATCGCTCAC
CCGTATTGAGGAAGGTCAGCAGACTCTCGACTTGCAGGAGCTAAGCCTAGCTCAACTTTGTACCGATTTATTGGAAATGG
CCGATGCGGTTGCCGAGTCAAACGAACAAACATTGAGTTTATCTTTGTTATCAGACCATACAGTGTTGGGTGATAAGTAC
TTATTATTCCAAGCTTTATATAATTTGGTCGACAATGCGATGAAATATTCTGGGCAGGGTGCGCGGATTGATATTATTCA
ATCAGGCGCACAAATTCAGATCCGTGATAATGGCCCTGGGATACCCGATGAGAGTAAAGATAAAGTGTTTGAGCGCCTAG
TGCGACTCGATCCTAGCCGACATTTACAGGGCACAGGATTAGGATTATCTATGGTCAAAGCGATTCTCTCACGGCACAAT
GCGCAAATTAAACTTAGCGATAATCATCCCGGCTTAGTCGTTACTATCCAATTCTAA

Upstream 100 bases:

>100_bases
CTTATTGAAACCGTAAGGGGAGCGGGTTATCGCTTACGCCAAAGGCATTAAGCCTTACCAAAGTAGCGCTTGGCGCATCA
CAATTGTCTTTTCAACCTTA

Downstream 100 bases:

>100_bases
GTCAATGATCCGCACTACCGCGTCGAGAGTTCCATGTACCAAATCATAGCTGATGAGGCTGATTTTCTTGTTATTTCAAA
ATTCCCTAAGGTGCACTTTC

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor protein saeS [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTVIIGALLFALYRQLIIEQQYQVTQYLESETQRYQQLALTVDRRSFAAQIRAADPQTALIAWRNSYDMVGALSFMPEGM
PMLPQTRDFPILTGGPDKLHILTGGLVMTRYGPVLIATRTDNLATLIDKFISAAATAVMLTIVLTLALGYLFSKAILRRL
VQYNRLSEQIERGHYDTRLPLSWRQDEFDMLALQFNNVLDILENNLMAVRGATDNIAHDLRTPLSHIRIGLEELATKSHD
DISEGCAILIEELDHCLATFDAMLSLTRIEEGQQTLDLQELSLAQLCTDLLEMADAVAESNEQTLSLSLLSDHTVLGDKY
LLFQALYNLVDNAMKYSGQGARIDIIQSGAQIQIRDNGPGIPDESKDKVFERLVRLDPSRHLQGTGLGLSMVKAILSRHN
AQIKLSDNHPGLVVTIQF

Sequences:

>Translated_418_residues
MTVIIGALLFALYRQLIIEQQYQVTQYLESETQRYQQLALTVDRRSFAAQIRAADPQTALIAWRNSYDMVGALSFMPEGM
PMLPQTRDFPILTGGPDKLHILTGGLVMTRYGPVLIATRTDNLATLIDKFISAAATAVMLTIVLTLALGYLFSKAILRRL
VQYNRLSEQIERGHYDTRLPLSWRQDEFDMLALQFNNVLDILENNLMAVRGATDNIAHDLRTPLSHIRIGLEELATKSHD
DISEGCAILIEELDHCLATFDAMLSLTRIEEGQQTLDLQELSLAQLCTDLLEMADAVAESNEQTLSLSLLSDHTVLGDKY
LLFQALYNLVDNAMKYSGQGARIDIIQSGAQIQIRDNGPGIPDESKDKVFERLVRLDPSRHLQGTGLGLSMVKAILSRHN
AQIKLSDNHPGLVVTIQF
>Mature_417_residues
TVIIGALLFALYRQLIIEQQYQVTQYLESETQRYQQLALTVDRRSFAAQIRAADPQTALIAWRNSYDMVGALSFMPEGMP
MLPQTRDFPILTGGPDKLHILTGGLVMTRYGPVLIATRTDNLATLIDKFISAAATAVMLTIVLTLALGYLFSKAILRRLV
QYNRLSEQIERGHYDTRLPLSWRQDEFDMLALQFNNVLDILENNLMAVRGATDNIAHDLRTPLSHIRIGLEELATKSHDD
ISEGCAILIEELDHCLATFDAMLSLTRIEEGQQTLDLQELSLAQLCTDLLEMADAVAESNEQTLSLSLLSDHTVLGDKYL
LFQALYNLVDNAMKYSGQGARIDIIQSGAQIQIRDNGPGIPDESKDKVFERLVRLDPSRHLQGTGLGLSMVKAILSRHNA
QIKLSDNHPGLVVTIQF

Specific function: Member of the two-component regulatory system saeR/saeS. Probably functions as a membrane-associated protein kinase that upon sensing the appropriate signal, autophosphorylates and in turn activates the cytosolic response regulator saeR [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=276, Percent_Identity=30.4347826086957, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1787894, Length=266, Percent_Identity=27.8195488721804, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786783, Length=331, Percent_Identity=24.773413897281, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788279, Length=270, Percent_Identity=28.8888888888889, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=29.7101449275362, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=27.4678111587983, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=208, Percent_Identity=27.4038461538462, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789403, Length=285, Percent_Identity=27.3684210526316, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786912, Length=199, Percent_Identity=32.1608040201005, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=210, Percent_Identity=30, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=25.8474576271186, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=212, Percent_Identity=27.8301886792453, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=208, Percent_Identity=25.9615384615385, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787374, Length=220, Percent_Identity=24.0909090909091, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=22.8187919463087, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46679; Mature: 46548

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIIGALLFALYRQLIIEQQYQVTQYLESETQRYQQLALTVDRRSFAAQIRAADPQTAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAWRNSYDMVGALSFMPEGMPMLPQTRDFPILTGGPDKLHILTGGLVMTRYGPVLIATRT
EEECCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEECCEEEECCCCEEEEECC
DNLATLIDKFISAAATAVMLTIVLTLALGYLFSKAILRRLVQYNRLSEQIERGHYDTRLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LSWRQDEFDMLALQFNNVLDILENNLMAVRGATDNIAHDLRTPLSHIRIGLEELATKSHD
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCC
DISEGCAILIEELDHCLATFDAMLSLTRIEEGQQTLDLQELSLAQLCTDLLEMADAVAES
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NEQTLSLSLLSDHTVLGDKYLLFQALYNLVDNAMKYSGQGARIDIIQSGAQIQIRDNGPG
CCCEEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCC
IPDESKDKVFERLVRLDPSRHLQGTGLGLSMVKAILSRHNAQIKLSDNHPGLVVTIQF
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCEEEEEEC
>Mature Secondary Structure 
TVIIGALLFALYRQLIIEQQYQVTQYLESETQRYQQLALTVDRRSFAAQIRAADPQTAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAWRNSYDMVGALSFMPEGMPMLPQTRDFPILTGGPDKLHILTGGLVMTRYGPVLIATRT
EEECCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEECCEEEECCCCEEEEECC
DNLATLIDKFISAAATAVMLTIVLTLALGYLFSKAILRRLVQYNRLSEQIERGHYDTRLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LSWRQDEFDMLALQFNNVLDILENNLMAVRGATDNIAHDLRTPLSHIRIGLEELATKSHD
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCC
DISEGCAILIEELDHCLATFDAMLSLTRIEEGQQTLDLQELSLAQLCTDLLEMADAVAES
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NEQTLSLSLLSDHTVLGDKYLLFQALYNLVDNAMKYSGQGARIDIIQSGAQIQIRDNGPG
CCCEEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCC
IPDESKDKVFERLVRLDPSRHLQGTGLGLSMVKAILSRHNAQIKLSDNHPGLVVTIQF
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA