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The map label for this gene is cusR [H]

Identifier: 120597082

GI number: 120597082

Start: 274267

End: 274944

Strand: Direct

Name: cusR [H]

Synonym: Sputw3181_0251

Alternate gene names: 120597082

Gene position: 274267-274944 (Clockwise)

Preceding gene: 120597081

Following gene: 120597083

Centisome position: 5.83

GC content: 45.13

Gene sequence:

>678_bases
ATGAAAATACTTTTAGTTGAAGATGATGCGACGACGATTGATTACATCGTCAAAGGATTTCTTGAGCAGGGGCACAATAT
CGAAACGGCGAATGATGGCCACCAAGGTTTATCTCTCGCCACAAGCTGTCAATATGATCTGGTGATCCTCGACCGAATGT
TACCCCAGCTTGATGGCTTAAAATTACTAGCGGCTTTAAGGGCTACGGGTAATCAAACCCCTGTGCTGATTTTGTCGGCT
TTAGCCCATGTTGATGAACGGGTTAAAGGTTTGCGGGCGGGCGGTGATGACTATATGACTAAGCCGTTTGCTTTTTCTGA
ACTGTTAGTACGCGCTGAAAAACTGATGCAACGTGGTCAATCCACGCCCATTTCGACCGACTTAGTGGTTGGTGGGCTCA
AGATGGAATTACTGACCCGCAATGTGACCTTGGACGGACAGGAGTTAATGTTGCAACCTAAAGAGTTTCAGTTGCTTAAG
TATTTGATGGAACATGCTAATCAGGTCATCAGCCGCACACTATTATTTGAAGCTGTGTGGGATTATCACTTTGACCCTCG
CACCAATGTAATTGATGTACATATTGCAAAATTACGGCGTAAATTTGAGGAGTTAGGTCACGGAGAGCTTATTGAAACCG
TAAGGGGAGCGGGTTATCGCTTACGCCAAAGGCATTAA

Upstream 100 bases:

>100_bases
ATAAATTGGCTTTTGATGTTGATAATTTACGTCAATTACCTTTACTCAAGTGGGATTAATGCCCGCAGCATCCGTGACGA
TTGGGGAGCGCAACATACAG

Downstream 100 bases:

>100_bases
GCCTTACCAAAGTAGCGCTTGGCGCATCACAATTGTCTTTTCAACCTTAGTGACTGTGATTATAGGTGCGTTGTTATTTG
CGCTTTATCGACAACTGATC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKILLVEDDATTIDYIVKGFLEQGHNIETANDGHQGLSLATSCQYDLVILDRMLPQLDGLKLLAALRATGNQTPVLILSA
LAHVDERVKGLRAGGDDYMTKPFAFSELLVRAEKLMQRGQSTPISTDLVVGGLKMELLTRNVTLDGQELMLQPKEFQLLK
YLMEHANQVISRTLLFEAVWDYHFDPRTNVIDVHIAKLRRKFEELGHGELIETVRGAGYRLRQRH

Sequences:

>Translated_225_residues
MKILLVEDDATTIDYIVKGFLEQGHNIETANDGHQGLSLATSCQYDLVILDRMLPQLDGLKLLAALRATGNQTPVLILSA
LAHVDERVKGLRAGGDDYMTKPFAFSELLVRAEKLMQRGQSTPISTDLVVGGLKMELLTRNVTLDGQELMLQPKEFQLLK
YLMEHANQVISRTLLFEAVWDYHFDPRTNVIDVHIAKLRRKFEELGHGELIETVRGAGYRLRQRH
>Mature_225_residues
MKILLVEDDATTIDYIVKGFLEQGHNIETANDGHQGLSLATSCQYDLVILDRMLPQLDGLKLLAALRATGNQTPVLILSA
LAHVDERVKGLRAGGDDYMTKPFAFSELLVRAEKLMQRGQSTPISTDLVVGGLKMELLTRNVTLDGQELMLQPKEFQLLK
YLMEHANQVISRTLLFEAVWDYHFDPRTNVIDVHIAKLRRKFEELGHGELIETVRGAGYRLRQRH

Specific function: Member of the two-component regulatory system CusS/CusR. Activates the expression of cusCFBA in response to increasing levels of copper ions, and probably silver ions [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=42.9864253393665, Blast_Score=193, Evalue=7e-51,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=42.9223744292237, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=41.1504424778761, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=36.6515837104072, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=32.4444444444444, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=32.8888888888889, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=37.0044052863436, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=29.2237442922374, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=33.1838565022422, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=28.5087719298246, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786911, Length=222, Percent_Identity=29.2792792792793, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=30.0884955752212, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1787229, Length=226, Percent_Identity=27.8761061946903, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788550, Length=116, Percent_Identity=33.6206896551724, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=29.5652173913043, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790437, Length=135, Percent_Identity=31.1111111111111, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=33.6134453781513, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787487, Length=101, Percent_Identity=37.6237623762376, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788713, Length=134, Percent_Identity=29.8507462686567, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=106, Percent_Identity=31.1320754716981, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR006291
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25420; Mature: 25420

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLVEDDATTIDYIVKGFLEQGHNIETANDGHQGLSLATSCQYDLVILDRMLPQLDGL
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCHHCHH
KLLAALRATGNQTPVLILSALAHVDERVKGLRAGGDDYMTKPFAFSELLVRAEKLMQRGQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
STPISTDLVVGGLKMELLTRNVTLDGQELMLQPKEFQLLKYLMEHANQVISRTLLFEAVW
CCCCCHHHEECCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYHFDPRTNVIDVHIAKLRRKFEELGHGELIETVRGAGYRLRQRH
HCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MKILLVEDDATTIDYIVKGFLEQGHNIETANDGHQGLSLATSCQYDLVILDRMLPQLDGL
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCHHCHH
KLLAALRATGNQTPVLILSALAHVDERVKGLRAGGDDYMTKPFAFSELLVRAEKLMQRGQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
STPISTDLVVGGLKMELLTRNVTLDGQELMLQPKEFQLLKYLMEHANQVISRTLLFEAVW
CCCCCHHHEECCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYHFDPRTNVIDVHIAKLRRKFEELGHGELIETVRGAGYRLRQRH
HCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]