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The map label for this gene is nisX1 [H]

Identifier: 120597066

GI number: 120597066

Start: 258218

End: 259267

Strand: Direct

Name: nisX1 [H]

Synonym: Sputw3181_0234

Alternate gene names: 120597066

Gene position: 258218-259267 (Clockwise)

Preceding gene: 120597065

Following gene: 120597067

Centisome position: 5.48

GC content: 47.43

Gene sequence:

>1050_bases
TTGCCTGAGCGCATAGCATTGATTGCCTTAATCCAAGAGGCGTATACCCAGGGTGCTCGCCTTTATAAGGCTTGTGCTGA
AACGGGCCTCAGTAAGCGTACCTATCGACGTTGGTATCGAGAAGGTCAAGTGCAAGCTGATTTAAGGCCAACAGCAGCTC
GACCAGAGCCAGCAAATAAACTCGAAGAACATGAGCGACAACAGATATTAAGTGTGTGCAATCAAGCGGAGTACGCCAGC
TTACCCCCGTCGCAAATTGTGCCAACATTATTAGATAACGGGATTTATATCGCCTCGGAATCCAGCTTCTACAGAGTGTT
AAATGCTCATGGTCAACTTAACCATCGTGGCCGCACTCAGGCTGCGGTCAATCGAAGCAAACCGTTGAGTTATCGGGCAG
ATGGGCCGAATCAAGTGTGGTCATGGGACATCACTTATTTAGCCTCAATCGTCAAAGGCCAGTTCTACTATTTGTACCTG
TTTGAAGATATTTACAGTAGAAAAATAGTGGGTTACGAAGTTCATGAGCAAGAAAGTGGGGAATATGCCGCTGCGCTAAT
CCAACGCTGTCTGTTACGCGAGCAATGCTTTAACACGCCATTAGTGCTGCATTCTGATAATGGGGCGCCGATGAAGTCAC
TGACCATGAAAGCCAAGCTGGAAGAGCTTGGTATTACGGCATCATTAAGTCGGCCGCGGGTGAGTAATGACAACCCCTTT
TCAGAGTCGTTATTTAAAACGTTGAAATACCGCCCACAGTGGCCGGCATCAGGTTTTAGCTGCTTAGCAACAGCAAGAGA
ATGGGTGGAAAAGTTTGTAACCTGGTACAACGATGAGCATAAACACAGCCAGCTAAATTTTGTCTCACCAGGGCAACGTC
ATGCACTGCAAGATGGCGCTATTTTAGCGAAGCGCAAGAAAGTGCTTGAGGCGGCGAAGGAAAGAAAACCGATGCGTTGG
TCTACAGAGATCAGAAATTGTGAAGCGGTTGGCGCTGTAACACTCAACCCAGATAAGGCACCAGAAGAAGGCGTAATAAA
TGCAGCTTAA

Upstream 100 bases:

>100_bases
GCTATAAGGAGAAAGCGCTGGCTGAAGCTGCTGCGTTACTGGTGCTCAGAAAAAAGCTCAATGCGCTCTGGGGGGACGAC
AGCGAGGAGAGCTAACCCCC

Downstream 100 bases:

>100_bases
TATTTAAACAAAGAGTGACAACTATCTTGAAAAACACCGACCTGAGGCAGCCTCTATTTTGGTTTTTGACAATACAGATC
AAGCCGGCAAGCCGATTCCT

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MPERIALIALIQEAYTQGARLYKACAETGLSKRTYRRWYREGQVQADLRPTAARPEPANKLEEHERQQILSVCNQAEYAS
LPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQAAVNRSKPLSYRADGPNQVWSWDITYLASIVKGQFYYLYL
FEDIYSRKIVGYEVHEQESGEYAAALIQRCLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSRPRVSNDNPF
SESLFKTLKYRPQWPASGFSCLATAREWVEKFVTWYNDEHKHSQLNFVSPGQRHALQDGAILAKRKKVLEAAKERKPMRW
STEIRNCEAVGAVTLNPDKAPEEGVINAA

Sequences:

>Translated_349_residues
MPERIALIALIQEAYTQGARLYKACAETGLSKRTYRRWYREGQVQADLRPTAARPEPANKLEEHERQQILSVCNQAEYAS
LPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQAAVNRSKPLSYRADGPNQVWSWDITYLASIVKGQFYYLYL
FEDIYSRKIVGYEVHEQESGEYAAALIQRCLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSRPRVSNDNPF
SESLFKTLKYRPQWPASGFSCLATAREWVEKFVTWYNDEHKHSQLNFVSPGQRHALQDGAILAKRKKVLEAAKERKPMRW
STEIRNCEAVGAVTLNPDKAPEEGVINAA
>Mature_348_residues
PERIALIALIQEAYTQGARLYKACAETGLSKRTYRRWYREGQVQADLRPTAARPEPANKLEEHERQQILSVCNQAEYASL
PPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQAAVNRSKPLSYRADGPNQVWSWDITYLASIVKGQFYYLYLF
EDIYSRKIVGYEVHEQESGEYAAALIQRCLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSRPRVSNDNPFS
ESLFKTLKYRPQWPASGFSCLATAREWVEKFVTWYNDEHKHSQLNFVSPGQRHALQDGAILAKRKKVLEAAKERKPMRWS
TEIRNCEAVGAVTLNPDKAPEEGVINAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787263, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786753, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786570, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786490, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 39651; Mature: 39520

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERIALIALIQEAYTQGARLYKACAETGLSKRTYRRWYREGQVQADLRPTAARPEPANK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCCHHH
LEEHERQQILSVCNQAEYASLPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCHH
AAVNRSKPLSYRADGPNQVWSWDITYLASIVKGQFYYLYLFEDIYSRKIVGYEVHEQESG
HHHCCCCCCEECCCCCCCEEEHHHHHHHHHHCCCEEEEEEEHHHHHCCEECEEECCCCCC
EYAAALIQRCLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSRPRVSNDNPF
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCH
SESLFKTLKYRPQWPASGFSCLATAREWVEKFVTWYNDEHKHSQLNFVSPGQRHALQDGA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCH
ILAKRKKVLEAAKERKPMRWSTEIRNCEAVGAVTLNPDKAPEEGVINAA
HHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PERIALIALIQEAYTQGARLYKACAETGLSKRTYRRWYREGQVQADLRPTAARPEPANK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCCHHH
LEEHERQQILSVCNQAEYASLPPSQIVPTLLDNGIYIASESSFYRVLNAHGQLNHRGRTQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCHH
AAVNRSKPLSYRADGPNQVWSWDITYLASIVKGQFYYLYLFEDIYSRKIVGYEVHEQESG
HHHCCCCCCEECCCCCCCEEEHHHHHHHHHHCCCEEEEEEEHHHHHCCEECEEECCCCCC
EYAAALIQRCLLREQCFNTPLVLHSDNGAPMKSLTMKAKLEELGITASLSRPRVSNDNPF
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCH
SESLFKTLKYRPQWPASGFSCLATAREWVEKFVTWYNDEHKHSQLNFVSPGQRHALQDGA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCH
ILAKRKKVLEAAKERKPMRWSTEIRNCEAVGAVTLNPDKAPEEGVINAA
HHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]