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The map label for this gene is fumB [H]

Identifier: 120597030

GI number: 120597030

Start: 222612

End: 224120

Strand: Direct

Name: fumB [H]

Synonym: Sputw3181_0198

Alternate gene names: 120597030

Gene position: 222612-224120 (Clockwise)

Preceding gene: 120597029

Following gene: 120597044

Centisome position: 4.73

GC content: 47.91

Gene sequence:

>1509_bases
ATGACAACTATAAAGAAAGTCGATTTTATTGACAGTATCGAAGATGCGCTTCAATACATCTCTTACTATCATCCACTGGA
TTTTATTCGTGCGATGGAAAAGGCGTACCACGCCGAGAAAAGTGTCGCGGCTAAAGATGCTATTGCTCAAATATTGATTA
ATTCGAGAATGGCTGCAGAAGGTAAGCGTCCTATTTGCCAAGATACGGGCATTGTGACTTGTTTTGTCAAAATTGGTATG
CAAGTGCAGTGGGATACCGATATGACGGTACAGGAAATGGTTGATGAAGGCACAAAAAGAGCCTACAACTGCGTAACTAA
TCCATTGCGGATGTCGGTTGTCTCCGATCCCGCTGGCGCGCGTAAAAATACGAATACCAATGGCCCTGCGGTCGTCCATA
TCGAAATGGTTCTCGGTGATAAGGTTGATATTATGATCGCGGCCAAGGGCGGCGGTTCAGAAAATAAGACCAAAATGGCA
ATGCTAAACCCATCGGATGATATTGCGGCATGGGTGGAAAAAACCGTACCGCTAATGGGCGCGGGTTGGTGTCCTCCTGG
CATGTTGGGTATTGGCATCGGTGGTACGGCAGAAAAAGCGGCATTGATGGCTAAAGAAGCGTTAATGGGCTCTGTTGATA
TCCACGAATTACAGGCGCGGGGATCACAGACTACCGAAGAAGAGCTGCGCTTAGATATTTTTGAACGTGTTAATAAACTC
GGTATTGGCGCGCAAGGTTTAGGTGGGATGACCACTGTTGTGGATATCAAAATCAAAACCGCACCGACACATGCGGCATC
CAAACCTGTCTGTATTATTCCGAATTGTGCGGCAACGCGGCACGTGCATTTTACGTTAGATGGCAATGGGCCTGCTAAGT
TAACGCCACCTAAGCTTGCCGATTGGCCAGAGGTAACCTGGGAAGTCGGTAAAAATGTGCGCCGAGTCGATGTAAATAAA
CTTACAAAAGCCGATATCGCTGAGTGGCGCTCTGGTGAAACCGTCCTACTCTCAGGTAAAATTCTTACCGGTCGTGATGC
TGCCCATAAACGCATTCAAGACATGCTTGCACGGGGAGAATCCTTCCCTGTTGATTTCCAGAATCGTTTTATCTACTACG
TCGGTCCAGTGGATGCTGTAGGTGATGAAGTGGTAGGCCCTGCAGGCCCAACGACCTCGACCCGCATGGATAAGTTCACC
GATATGATGCTAGAGCAAGCGGGCTTAATGGGTATGATTGGTAAATCGGAACGTGGTGCTGCTACGGTTGAGTCGATTAA
GAAAAATCAAGCCGTGTATTTGATGGCTGTCGGTGGCGCCGCATACTTAGTGGCAAAAGCGATTAAGTCAGCCAAGATCC
TCGCCTTTGAAGATCTTGGAATGGAAGCCATTTACGAGTTTGATGTTGAAGATATGCCAGTGACTGTCGCCGTGGACAGC
CTCGGCGGAAATGTGCACGAAACCGGGCCACAAACCTGGAAACTCAAAATTGCCGAAATGACTGCCTAA

Upstream 100 bases:

>100_bases
GGAATTTGGTGGAAAGTGATCGGGCTATATTAATCATGTGTCGGGCGGGGTAACTCTTTCCCCCGCTCTAGCTTGCTGTA
AATAAATACAAGGGAACGTT

Downstream 100 bases:

>100_bases
TATATTTACCCCTGCAAATTAGGCGGTTAGAAGCAATACTAACGGTAACCAACAGACAAACATCTGTTGGTTACCGTTTT
TTATTAGCCAAGCAAATCCA

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTTIKKVDFIDSIEDALQYISYYHPLDFIRAMEKAYHAEKSVAAKDAIAQILINSRMAAEGKRPICQDTGIVTCFVKIGM
QVQWDTDMTVQEMVDEGTKRAYNCVTNPLRMSVVSDPAGARKNTNTNGPAVVHIEMVLGDKVDIMIAAKGGGSENKTKMA
MLNPSDDIAAWVEKTVPLMGAGWCPPGMLGIGIGGTAEKAALMAKEALMGSVDIHELQARGSQTTEEELRLDIFERVNKL
GIGAQGLGGMTTVVDIKIKTAPTHAASKPVCIIPNCAATRHVHFTLDGNGPAKLTPPKLADWPEVTWEVGKNVRRVDVNK
LTKADIAEWRSGETVLLSGKILTGRDAAHKRIQDMLARGESFPVDFQNRFIYYVGPVDAVGDEVVGPAGPTTSTRMDKFT
DMMLEQAGLMGMIGKSERGAATVESIKKNQAVYLMAVGGAAYLVAKAIKSAKILAFEDLGMEAIYEFDVEDMPVTVAVDS
LGGNVHETGPQTWKLKIAEMTA

Sequences:

>Translated_502_residues
MTTIKKVDFIDSIEDALQYISYYHPLDFIRAMEKAYHAEKSVAAKDAIAQILINSRMAAEGKRPICQDTGIVTCFVKIGM
QVQWDTDMTVQEMVDEGTKRAYNCVTNPLRMSVVSDPAGARKNTNTNGPAVVHIEMVLGDKVDIMIAAKGGGSENKTKMA
MLNPSDDIAAWVEKTVPLMGAGWCPPGMLGIGIGGTAEKAALMAKEALMGSVDIHELQARGSQTTEEELRLDIFERVNKL
GIGAQGLGGMTTVVDIKIKTAPTHAASKPVCIIPNCAATRHVHFTLDGNGPAKLTPPKLADWPEVTWEVGKNVRRVDVNK
LTKADIAEWRSGETVLLSGKILTGRDAAHKRIQDMLARGESFPVDFQNRFIYYVGPVDAVGDEVVGPAGPTTSTRMDKFT
DMMLEQAGLMGMIGKSERGAATVESIKKNQAVYLMAVGGAAYLVAKAIKSAKILAFEDLGMEAIYEFDVEDMPVTVAVDS
LGGNVHETGPQTWKLKIAEMTA
>Mature_501_residues
TTIKKVDFIDSIEDALQYISYYHPLDFIRAMEKAYHAEKSVAAKDAIAQILINSRMAAEGKRPICQDTGIVTCFVKIGMQ
VQWDTDMTVQEMVDEGTKRAYNCVTNPLRMSVVSDPAGARKNTNTNGPAVVHIEMVLGDKVDIMIAAKGGGSENKTKMAM
LNPSDDIAAWVEKTVPLMGAGWCPPGMLGIGIGGTAEKAALMAKEALMGSVDIHELQARGSQTTEEELRLDIFERVNKLG
IGAQGLGGMTTVVDIKIKTAPTHAASKPVCIIPNCAATRHVHFTLDGNGPAKLTPPKLADWPEVTWEVGKNVRRVDVNKL
TKADIAEWRSGETVLLSGKILTGRDAAHKRIQDMLARGESFPVDFQNRFIYYVGPVDAVGDEVVGPAGPTTSTRMDKFTD
MMLEQAGLMGMIGKSERGAATVESIKKNQAVYLMAVGGAAYLVAKAIKSAKILAFEDLGMEAIYEFDVEDMPVTVAVDSL
GGNVHETGPQTWKLKIAEMTA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=473, Percent_Identity=32.1353065539112, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787897, Length=474, Percent_Identity=32.9113924050633, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789442, Length=268, Percent_Identity=28.3582089552239, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789443, Length=178, Percent_Identity=29.2134831460674, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54219; Mature: 54088

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKKVDFIDSIEDALQYISYYHPLDFIRAMEKAYHAEKSVAAKDAIAQILINSRMAAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKRPICQDTGIVTCFVKIGMQVQWDTDMTVQEMVDEGTKRAYNCVTNPLRMSVVSDPAGA
CCCCCCCCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
RKNTNTNGPAVVHIEMVLGDKVDIMIAAKGGGSENKTKMAMLNPSDDIAAWVEKTVPLMG
CCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCEE
AGWCPPGMLGIGIGGTAEKAALMAKEALMGSVDIHELQARGSQTTEEELRLDIFERVNKL
CCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
GIGAQGLGGMTTVVDIKIKTAPTHAASKPVCIIPNCAATRHVHFTLDGNGPAKLTPPKLA
CCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCC
DWPEVTWEVGKNVRRVDVNKLTKADIAEWRSGETVLLSGKILTGRDAAHKRIQDMLARGE
CCCHHHHHHCCCEEEECHHHHHHHHHHHCCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
SFPVDFQNRFIYYVGPVDAVGDEVVGPAGPTTSTRMDKFTDMMLEQAGLMGMIGKSERGA
CCCEECCCCEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCH
ATVESIKKNQAVYLMAVGGAAYLVAKAIKSAKILAFEDLGMEAIYEFDVEDMPVTVAVDS
HHHHHHHCCCEEEEEEECCHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCEEEEEEC
LGGNVHETGPQTWKLKIAEMTA
CCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
TTIKKVDFIDSIEDALQYISYYHPLDFIRAMEKAYHAEKSVAAKDAIAQILINSRMAAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKRPICQDTGIVTCFVKIGMQVQWDTDMTVQEMVDEGTKRAYNCVTNPLRMSVVSDPAGA
CCCCCCCCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
RKNTNTNGPAVVHIEMVLGDKVDIMIAAKGGGSENKTKMAMLNPSDDIAAWVEKTVPLMG
CCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCEE
AGWCPPGMLGIGIGGTAEKAALMAKEALMGSVDIHELQARGSQTTEEELRLDIFERVNKL
CCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
GIGAQGLGGMTTVVDIKIKTAPTHAASKPVCIIPNCAATRHVHFTLDGNGPAKLTPPKLA
CCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCC
DWPEVTWEVGKNVRRVDVNKLTKADIAEWRSGETVLLSGKILTGRDAAHKRIQDMLARGE
CCCHHHHHHCCCEEEECHHHHHHHHHHHCCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
SFPVDFQNRFIYYVGPVDAVGDEVVGPAGPTTSTRMDKFTDMMLEQAGLMGMIGKSERGA
CCCEECCCCEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCH
ATVESIKKNQAVYLMAVGGAAYLVAKAIKSAKILAFEDLGMEAIYEFDVEDMPVTVAVDS
HHHHHHHCCCEEEEEEECCHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCEEEEEEC
LGGNVHETGPQTWKLKIAEMTA
CCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]