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The map label for this gene is ykoW [H]

Identifier: 120596868

GI number: 120596868

Start: 44067

End: 46517

Strand: Direct

Name: ykoW [H]

Synonym: Sputw3181_0035

Alternate gene names: 120596868

Gene position: 44067-46517 (Clockwise)

Preceding gene: 120596866

Following gene: 120596871

Centisome position: 0.94

GC content: 40.64

Gene sequence:

>2451_bases
ATGCTTAGAAAACTTTCACTCGCAGTTCTAATTCCTCTTTGTATGCTAATACTTTATTTGGTGTTAGTCATAGGAGAGTA
TTTGTATGAGAGTGATCAAATACGCAGTGAGCTCGCTGGACGTCAGGTTACTCAAATAAAACAACAGCTTTTGAGAATGC
AGGCGGTTGTACAATCGGCACAAGTTTTACAGGATATTGAGCGTATAGAACAAGAGGTATCATTGGCTGCACTGGATATG
AACACAATGGTGTATATATTGGTGGATATGGATAGTCGGATCCGCTTTGCTAATCATACCGTTTGGCGCGATAGTAATGC
TATTCAAGTTATTGATGGTTATGATGTTGTTCGACATCATACCGTTGCTCAATCCTCTCTCCCGCAAGTATCTGTCAATA
TGGAGCGTTTAACGATTCAGGCTTATTATCCTGTTATTTCTCAATACCCTGGTGCAGTTGAATTAATTTACCTCGAATCT
GACTTGGCTCCTTTAGTTTCAGAAGCCTCTGCTAAGTTACAACAGCGATTTATCCGTGTATGGGGCTTGGGTGGATTGCT
ATTGATTGGTTTTACTCTCATTTTATATTTTTTATTAATTAAGCCATTTCGGATTTTAAGTGAAAGTGCAAAGCATATAG
GTACACCTGAATTTTCAACTCAGATCCCTTGGAGTTCTTCAGAGGTTTTGTCACTGCAAAGCAGTTTACAGGAGGTACAT
GCCCGTTTGGGACGTGCAGTAAAACAGCTAAATGACAGTGAGCAGCGTTGGCTATTTGCCGTTGAGGGATCTCGTAATGG
TATTTGGGATTGGGATATTAGCACTGGTGAAGTATTTCTTTCCGATCGTTGGAAAGAGATGATTGGTTATGCCCCTGATG
AATTAGAGTGTGTTTTTAAGACTTGGGAGTCGCGGCTGCATCCGGAAGACCGAGAGACTGTTTTAGATTGTTTGCAGGAA
TATATTAGCGGTAAAAGTAAAGAGTTTGAGAGTGTCCATCGACTGCGGCATCGTGATGGTCATTATATATGGGTGCTCGA
TCGTGGCATGCTTGTTGATTGGGATCATTTAGGCCGTCCAATGCGGATGATAGGTATCCATGTTGATGTATCTGAAAGTG
AGAAAAACCATGCAGCAATTGCAGAGTTAGTTGAACATTCAGTTGCAGGGCATCGAATGTTACCAGCAGTATTCATGGAA
CGTTTGTCCCAATTTCTGACTCAAGAAGATAATTCTGGTCATTGGGGAGGATTATTTTTTATTGAGGTTGAGAGTTTAGG
GGTAATGAACTCGCTAACTTCACATGAAGCTGAGCGTTTATTTACACAGATTGGCGCGAGACTCTCGAGCTACTTCACAG
ACAATATTATTGTTGGACATTTGGGGTTAGGGCGTTTCACCTTGCTTGCTAAAGAATTGTCGATTGATGCAGAAGTGGCT
GCACGTCGAACTCTAGCCCTAGCGACTGAGTTGAGGCAAATCATTGCAAGACCATTTCATTACAGAGATCATCACTTTGA
ATTAAATGCCAATGTCGGTATTTATCTGCTCGATCCAGTAGAAACATTAGAGCCCCTACTTGTGATACGCCGCGCCGAGC
TAGCCCAAAAACATGCAAAACTGTTAGGGCAATGCGGTTGCGCTTTTTATCATTCGGAGTTAGATGTACGTCAGAGCCGT
GATGAGCTGTTACAACATGAGCTTAAGTTAGCGATAGAACATGAAAAGCTATCATTAATGTTTCAACCGATTGTGAATGT
AGCGGGCGAAATTCTCTCTGCAGAAGTGTTATCACGTTGGTATTTAGCTAATGGTGATGTTGTTCCTCCTTCTGAGTTTA
TTGCTCTTGCTGAACGTTGTGGTTTGATTGCAGGGTTAGATTTAAGCGTTGCTAAACGAATATGTATATTATTACAGCAA
GCAGATGAGCAGGGTTTATCTCTTCCTCGTATAACGATCAATATTAGCGCGTTATCATTTTGTCAGATTGATTTTATTGA
GCATTTAATTGCTTTGATTCGTACGTATTCAATTCGTACTAATCAACTTGGCATTGAATTAAATGAATCTGCACTACTCT
TACAAGATGCTTTTGTGAATGAACGTATTACCCAGTTAGTGGAGATTGGGGTGCCAGTCATTCTAGATAACTTTGGTTCA
GGTCACGCTGCGTTAAGCTGCTTAAATCAGTATCCGATAAGCGAAGTAAAGTTAGATCTGAGTTGTGTCGCTTCTATTAC
CTCGACGCAGATATGGCAAAAAGCATTAATTCAATCAGTATTACCATTCGACCTATGCTTAGTGGCTAAAGGTATAGAGT
CAGCACAGCAACAACAGATATTTACGGCATTAGGTTGCCATGCTTATCAAGGCTATTATTATAGCCGAGCACTAAATTTT
GCTGATTTTAAACAACTTTTCCATTCGAATTCTTTGTTGCGTAGCGTTTAA

Upstream 100 bases:

>100_bases
CAGAAGATCTCGATAACTTCGTTAAGTCCTTTTGCTTAGTATCCTATTTCCCGTTAGCATACGAATAATTCATTGTTCAG
TAGAAGGCTGCCTAGCGCAG

Downstream 100 bases:

>100_bases
AAAAATCGAGTGCATAGTTTAAGGCTTGGTTTCGGCAGGCGTCTTTTTCCATAAAAATCTCATGGGCAGCCCCAGCGATT
ACTTTCAATTGGCAATGGGT

Product: diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 816; Mature: 816

Protein sequence:

>816_residues
MLRKLSLAVLIPLCMLILYLVLVIGEYLYESDQIRSELAGRQVTQIKQQLLRMQAVVQSAQVLQDIERIEQEVSLAALDM
NTMVYILVDMDSRIRFANHTVWRDSNAIQVIDGYDVVRHHTVAQSSLPQVSVNMERLTIQAYYPVISQYPGAVELIYLES
DLAPLVSEASAKLQQRFIRVWGLGGLLLIGFTLILYFLLIKPFRILSESAKHIGTPEFSTQIPWSSSEVLSLQSSLQEVH
ARLGRAVKQLNDSEQRWLFAVEGSRNGIWDWDISTGEVFLSDRWKEMIGYAPDELECVFKTWESRLHPEDRETVLDCLQE
YISGKSKEFESVHRLRHRDGHYIWVLDRGMLVDWDHLGRPMRMIGIHVDVSESEKNHAAIAELVEHSVAGHRMLPAVFME
RLSQFLTQEDNSGHWGGLFFIEVESLGVMNSLTSHEAERLFTQIGARLSSYFTDNIIVGHLGLGRFTLLAKELSIDAEVA
ARRTLALATELRQIIARPFHYRDHHFELNANVGIYLLDPVETLEPLLVIRRAELAQKHAKLLGQCGCAFYHSELDVRQSR
DELLQHELKLAIEHEKLSLMFQPIVNVAGEILSAEVLSRWYLANGDVVPPSEFIALAERCGLIAGLDLSVAKRICILLQQ
ADEQGLSLPRITINISALSFCQIDFIEHLIALIRTYSIRTNQLGIELNESALLLQDAFVNERITQLVEIGVPVILDNFGS
GHAALSCLNQYPISEVKLDLSCVASITSTQIWQKALIQSVLPFDLCLVAKGIESAQQQQIFTALGCHAYQGYYYSRALNF
ADFKQLFHSNSLLRSV

Sequences:

>Translated_816_residues
MLRKLSLAVLIPLCMLILYLVLVIGEYLYESDQIRSELAGRQVTQIKQQLLRMQAVVQSAQVLQDIERIEQEVSLAALDM
NTMVYILVDMDSRIRFANHTVWRDSNAIQVIDGYDVVRHHTVAQSSLPQVSVNMERLTIQAYYPVISQYPGAVELIYLES
DLAPLVSEASAKLQQRFIRVWGLGGLLLIGFTLILYFLLIKPFRILSESAKHIGTPEFSTQIPWSSSEVLSLQSSLQEVH
ARLGRAVKQLNDSEQRWLFAVEGSRNGIWDWDISTGEVFLSDRWKEMIGYAPDELECVFKTWESRLHPEDRETVLDCLQE
YISGKSKEFESVHRLRHRDGHYIWVLDRGMLVDWDHLGRPMRMIGIHVDVSESEKNHAAIAELVEHSVAGHRMLPAVFME
RLSQFLTQEDNSGHWGGLFFIEVESLGVMNSLTSHEAERLFTQIGARLSSYFTDNIIVGHLGLGRFTLLAKELSIDAEVA
ARRTLALATELRQIIARPFHYRDHHFELNANVGIYLLDPVETLEPLLVIRRAELAQKHAKLLGQCGCAFYHSELDVRQSR
DELLQHELKLAIEHEKLSLMFQPIVNVAGEILSAEVLSRWYLANGDVVPPSEFIALAERCGLIAGLDLSVAKRICILLQQ
ADEQGLSLPRITINISALSFCQIDFIEHLIALIRTYSIRTNQLGIELNESALLLQDAFVNERITQLVEIGVPVILDNFGS
GHAALSCLNQYPISEVKLDLSCVASITSTQIWQKALIQSVLPFDLCLVAKGIESAQQQQIFTALGCHAYQGYYYSRALNF
ADFKQLFHSNSLLRSV
>Mature_816_residues
MLRKLSLAVLIPLCMLILYLVLVIGEYLYESDQIRSELAGRQVTQIKQQLLRMQAVVQSAQVLQDIERIEQEVSLAALDM
NTMVYILVDMDSRIRFANHTVWRDSNAIQVIDGYDVVRHHTVAQSSLPQVSVNMERLTIQAYYPVISQYPGAVELIYLES
DLAPLVSEASAKLQQRFIRVWGLGGLLLIGFTLILYFLLIKPFRILSESAKHIGTPEFSTQIPWSSSEVLSLQSSLQEVH
ARLGRAVKQLNDSEQRWLFAVEGSRNGIWDWDISTGEVFLSDRWKEMIGYAPDELECVFKTWESRLHPEDRETVLDCLQE
YISGKSKEFESVHRLRHRDGHYIWVLDRGMLVDWDHLGRPMRMIGIHVDVSESEKNHAAIAELVEHSVAGHRMLPAVFME
RLSQFLTQEDNSGHWGGLFFIEVESLGVMNSLTSHEAERLFTQIGARLSSYFTDNIIVGHLGLGRFTLLAKELSIDAEVA
ARRTLALATELRQIIARPFHYRDHHFELNANVGIYLLDPVETLEPLLVIRRAELAQKHAKLLGQCGCAFYHSELDVRQSR
DELLQHELKLAIEHEKLSLMFQPIVNVAGEILSAEVLSRWYLANGDVVPPSEFIALAERCGLIAGLDLSVAKRICILLQQ
ADEQGLSLPRITINISALSFCQIDFIEHLIALIRTYSIRTNQLGIELNESALLLQDAFVNERITQLVEIGVPVILDNFGS
GHAALSCLNQYPISEVKLDLSCVASITSTQIWQKALIQSVLPFDLCLVAKGIESAQQQQIFTALGCHAYQGYYYSRALNF
ADFKQLFHSNSLLRSV

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=318, Percent_Identity=30.5031446540881, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787541, Length=438, Percent_Identity=26.2557077625571, Blast_Score=133, Evalue=5e-32,
Organism=Escherichia coli, GI87081743, Length=249, Percent_Identity=30.5220883534137, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI87081845, Length=284, Percent_Identity=27.8169014084507, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790496, Length=250, Percent_Identity=27.2, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI226510982, Length=422, Percent_Identity=25.3554502369668, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082096, Length=301, Percent_Identity=27.5747508305648, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788849, Length=334, Percent_Identity=24.8502994011976, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786507, Length=241, Percent_Identity=26.1410788381743, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788502, Length=250, Percent_Identity=27.2, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1787055, Length=243, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 92396; Mature: 92396

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKLSLAVLIPLCMLILYLVLVIGEYLYESDQIRSELAGRQVTQIKQQLLRMQAVVQSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QVLQDIERIEQEVSLAALDMNTMVYILVDMDSRIRFANHTVWRDSNAIQVIDGYDVVRHH
HHHHHHHHHHHHHHHHHEECCEEEEEEEECCCCEEECCCEEEECCCCEEEECCHHHHHHH
TVAQSSLPQVSVNMERLTIQAYYPVISQYPGAVELIYLESDLAPLVSEASAKLQQRFIRV
HHHHHCCCCEEECHHHEEEEEEHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
WGLGGLLLIGFTLILYFLLIKPFRILSESAKHIGTPEFSTQIPWSSSEVLSLQSSLQEVH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ARLGRAVKQLNDSEQRWLFAVEGSRNGIWDWDISTGEVFLSDRWKEMIGYAPDELECVFK
HHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHCCCCHHHHHHHH
TWESRLHPEDRETVLDCLQEYISGKSKEFESVHRLRHRDGHYIWVLDRGMLVDWDHLGRP
HHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHCCCC
MRMIGIHVDVSESEKNHAAIAELVEHSVAGHRMLPAVFMERLSQFLTQEDNSGHWGGLFF
EEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IEVESLGVMNSLTSHEAERLFTQIGARLSSYFTDNIIVGHLGLGRFTLLAKELSIDAEVA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHH
ARRTLALATELRQIIARPFHYRDHHFELNANVGIYLLDPVETLEPLLVIRRAELAQKHAK
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
LLGQCGCAFYHSELDVRQSRDELLQHELKLAIEHEKLSLMFQPIVNVAGEILSAEVLSRW
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLANGDVVPPSEFIALAERCGLIAGLDLSVAKRICILLQQADEQGLSLPRITINISALSF
HHCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHH
CQIDFIEHLIALIRTYSIRTNQLGIELNESALLLQDAFVNERITQLVEIGVPVILDNFGS
HHHHHHHHHHHHHHHHCCEECCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GHAALSCLNQYPISEVKLDLSCVASITSTQIWQKALIQSVLPFDLCLVAKGIESAQQQQI
HHHHHHHHHHCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FTALGCHAYQGYYYSRALNFADFKQLFHSNSLLRSV
HHHHHHHHHCCCHHHHHCCHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MLRKLSLAVLIPLCMLILYLVLVIGEYLYESDQIRSELAGRQVTQIKQQLLRMQAVVQSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QVLQDIERIEQEVSLAALDMNTMVYILVDMDSRIRFANHTVWRDSNAIQVIDGYDVVRHH
HHHHHHHHHHHHHHHHHEECCEEEEEEEECCCCEEECCCEEEECCCCEEEECCHHHHHHH
TVAQSSLPQVSVNMERLTIQAYYPVISQYPGAVELIYLESDLAPLVSEASAKLQQRFIRV
HHHHHCCCCEEECHHHEEEEEEHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
WGLGGLLLIGFTLILYFLLIKPFRILSESAKHIGTPEFSTQIPWSSSEVLSLQSSLQEVH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ARLGRAVKQLNDSEQRWLFAVEGSRNGIWDWDISTGEVFLSDRWKEMIGYAPDELECVFK
HHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHCCCCHHHHHHHH
TWESRLHPEDRETVLDCLQEYISGKSKEFESVHRLRHRDGHYIWVLDRGMLVDWDHLGRP
HHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHCCCC
MRMIGIHVDVSESEKNHAAIAELVEHSVAGHRMLPAVFMERLSQFLTQEDNSGHWGGLFF
EEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IEVESLGVMNSLTSHEAERLFTQIGARLSSYFTDNIIVGHLGLGRFTLLAKELSIDAEVA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHH
ARRTLALATELRQIIARPFHYRDHHFELNANVGIYLLDPVETLEPLLVIRRAELAQKHAK
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
LLGQCGCAFYHSELDVRQSRDELLQHELKLAIEHEKLSLMFQPIVNVAGEILSAEVLSRW
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLANGDVVPPSEFIALAERCGLIAGLDLSVAKRICILLQQADEQGLSLPRITINISALSF
HHCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHH
CQIDFIEHLIALIRTYSIRTNQLGIELNESALLLQDAFVNERITQLVEIGVPVILDNFGS
HHHHHHHHHHHHHHHHCCEECCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GHAALSCLNQYPISEVKLDLSCVASITSTQIWQKALIQSVLPFDLCLVAKGIESAQQQQI
HHHHHHHHHHCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FTALGCHAYQGYYYSRALNFADFKQLFHSNSLLRSV
HHHHHHHHHCCCHHHHHCCHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]