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The map label for this gene is recF

Identifier: 120596836

GI number: 120596836

Start: 2853

End: 3935

Strand: Direct

Name: recF

Synonym: Sputw3181_0003

Alternate gene names: 120596836

Gene position: 2853-3935 (Clockwise)

Preceding gene: 120596835

Following gene: 120596837

Centisome position: 0.06

GC content: 44.51

Gene sequence:

>1083_bases
ATGAGTTTAACCCGTCTTAATATTGAAGCTTTTCGCAATATTCAGTTTGCACAGTTAATCCCTGCGCCAGGGATTAACGT
GATTTATGGCCAAAATGGCAGCGGAAAAACCAGTATTTTAGAAGCCATTTATTTCCTCGGTATGGGGCGTTCGTTTCGCA
GCCATTTATCGCAGCGGGTGATTAATAACGACAACGATAAGTTGACTCTATTTGCGACGTTAAACTTAGCGCGCGGTGAC
AGTAAAATCGGTTTGCGCCGATTTCGTAGCGGTGAGACTGAAGTTAAAATTGATGGCGAAAAGGTCAAACGGCTATCGAC
CTTAGCCGAAACCTTGCCTATTCAAGTGATCACACCAGAAAGCTTTTCGTTACTGTTTGAAGGCCCAAAATCCCGTCGAC
AATTTATCGATTGGGGCGCATTTCATGCCGATCCGCAGTTTTATGGTGCTTGGACGAATGTTCGTCGTGTTCTTAAGCAG
AGAAATCAATTGCTTAGGAATGGTGCTGTTTACACTCATATTCAATTTTGGGACCAAGAATTTGTGCGTTATGCCGAGCA
GGTCACCGAAATACGAAACCATTATGTAGACTCGTTAAATGGGCTACTTAAGGGTATAATCGGGGAGTTTTTACCGAGTG
TAGATGTGAAGGTTTCATTTACTCGCGGCTGGGATAGTAAGACGGATTTTGCAGAACTACTCGAAAACCAATATTCCAGA
GATTTGGCCACAGGTCATACCGTCAGCGGTCCCCATAAAGCTGACCTTAGACTTCGTGTAGGCAATTTGCCGGCGCAGGA
TGCCCTGTCCCGTGGTCAATTGAAATTATTAGTCTGTGCACTGCGTATTGCACAGGGAAAGTTGCTCAAACAACAAATAG
ATAAACACAGTATTTATCTTGTGGATGATCTTCCATCCGAGTTGGATGCACAGCATAGGCAGCTATTGCTTAAGCAGTTA
ACGGATACCGGAGCACAAGTATTTGTGACCGCCATCGATCCTGCTGCAATAGTCGATTCGTTACACACGCCTCCCAATAG
GATGTTTCATGTGGAACAGGGACGTGTAACGGTAGTTGAATAA

Upstream 100 bases:

>100_bases
ATCCATCATTGGTATGGTGCGGCTTCACTAAGGTCAGTGCGGTATGATAGCTATTATGAATTTCTGTACTGTGCCGATTG
CTCGGCTCTATGGGTAATGC

Downstream 100 bases:

>100_bases
CCATTGAGAGAATAATATGTCAGAGAATAGTTACGATTCTTCGAGTATTAAGGTCCTTAAGGGCCTTGATGCAGTACGTA
AGAGACCTGGGATGTATATT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSLTRLNIEAFRNIQFAQLIPAPGINVIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVINNDNDKLTLFATLNLARGD
SKIGLRRFRSGETEVKIDGEKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQ
RNQLLRNGAVYTHIQFWDQEFVRYAEQVTEIRNHYVDSLNGLLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
DLATGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAQHRQLLLKQL
TDTGAQVFVTAIDPAAIVDSLHTPPNRMFHVEQGRVTVVE

Sequences:

>Translated_360_residues
MSLTRLNIEAFRNIQFAQLIPAPGINVIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVINNDNDKLTLFATLNLARGD
SKIGLRRFRSGETEVKIDGEKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQ
RNQLLRNGAVYTHIQFWDQEFVRYAEQVTEIRNHYVDSLNGLLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
DLATGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAQHRQLLLKQL
TDTGAQVFVTAIDPAAIVDSLHTPPNRMFHVEQGRVTVVE
>Mature_359_residues
SLTRLNIEAFRNIQFAQLIPAPGINVIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVINNDNDKLTLFATLNLARGDS
KIGLRRFRSGETEVKIDGEKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQR
NQLLRNGAVYTHIQFWDQEFVRYAEQVTEIRNHYVDSLNGLLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSRD
LATGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAQHRQLLLKQLT
DTGAQVFVTAIDPAAIVDSLHTPPNRMFHVEQGRVTVVE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=45.6582633053221, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40646; Mature: 40515

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRLNIEAFRNIQFAQLIPAPGINVIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCCEEEEHHHHHCCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
INNDNDKLTLFATLNLARGDSKIGLRRFRSGETEVKIDGEKVKRLSTLAETLPIQVITPE
HCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQRNQLLRNGAVYTHIQFWDQE
CCEEEECCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHH
FVRYAEQVTEIRNHYVDSLNGLLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
DLATGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYL
HHCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDDLPSELDAQHRQLLLKQLTDTGAQVFVTAIDPAAIVDSLHTPPNRMFHVEQGRVTVVE
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCEEEEECCCEEEEC
>Mature Secondary Structure 
SLTRLNIEAFRNIQFAQLIPAPGINVIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCEEEEHHHHHCCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
INNDNDKLTLFATLNLARGDSKIGLRRFRSGETEVKIDGEKVKRLSTLAETLPIQVITPE
HCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQRNQLLRNGAVYTHIQFWDQE
CCEEEECCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHH
FVRYAEQVTEIRNHYVDSLNGLLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
DLATGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYL
HHCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDDLPSELDAQHRQLLLKQLTDTGAQVFVTAIDPAAIVDSLHTPPNRMFHVEQGRVTVVE
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA